LOCUS Exported 10331 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Cas9 and gRNA expression plasmid for P. falciparum; no selection in parasites.. ACCESSION . VERSION . KEYWORDS pAIO3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10331) AUTHORS Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, Goldberg DE, Beck JR TITLE EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole. JOURNAL Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168. PUBMED 31990132 REFERENCE 2 (bases 1 to 10331) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10331 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 1165..1240 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS complement(2610..2633) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS complement(2634..2654) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(2661..2681) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(2682..6785) /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" primer_bind complement(7893..7912) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8012..8034 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8072..8090) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8158..8262 /gene="bla" /label=AmpR promoter CDS 8263..9123 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8481..8500) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9294..9882 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9783..9802 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10036..10053 /label=L4440 /note="L4440 vector, forward primer" protein_bind 10170..10191 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10206..10236 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10244..10260 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10249..10271 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10268..10284 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10268..10284 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 10302..10320 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 10302..10319 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 gcggccgcaa cggtaaaaat aataacacga attatcacaa taatttttca catatgtata 61 tatatatata tattatcaaa gggacaaccg tttcaagaaa gcaaatgtta ttattttgtt 121 tttattctaa tgtatgtaag ccaaaaacca ataaaaaagt aataattaaa aaggaaataa 181 aataaatata catatgtaat tatacttata taataagtat gtatattttt tattattttt 241 tttttttaat gctatttatt tattattgca tctttttatt taatttttgt atttttttat 301 ataattcctt tatttcttta tttctttctt tatttctttt tttttttttt tttttttttt 361 tttttccttt aattaaactt taaaagacgt tataaatttt aaacatattt tataaataaa 421 aaaaaaaaaa aaaaagacga aataataaaa taaacgaaga taaagtaata ttttgttatt 481 atgtcacata gtatgtgtac tacaattatt tatggatgca aattattaaa ttggtagtta 541 ttatatacat acatgttttt ttttttgtgt gtattaaaag gaactattat ataaatagaa 601 caatttcatg acatttggat ttctacacat cttgaggttt tacaataata tcttcttttt 661 ttttttttct cttctatata tgaaatatat tgtaaaaagg tacaatatat ataatattta 721 tatactatga aaaaaaaaaa atatatacat aaactaataa taatttattc taatttaata 781 taaaaaaaaa ttcttgcttg ttcagataaa taaaagaaat ttatataaat agctatattt 841 tttttttttt tttttttttt tttttctaat tataaatata attattcatt taaaaaattt 901 taaatattta aatatttcat aaattaaaaa aaaaaaaaaa aaaactatta atataataaa 961 cttttatttt tactgtaata taatttttta taatgtaaaa ataaagggta aattattatt 1021 aaaaaatgta tatgttatgt atatataaca taatatatta taatatatat atatatatat 1081 atatatatat aatattagag taaccaaaat gcataatttt tcctatatgc acatatttca 1141 tattaagtat ataatattct taaggtttta gagctagaaa tagcaagtta aaataaggct 1201 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttatta tttcctataa 1261 aataatatat atatatatat atatatatat atatatatat tttaaatata atgaagtttt 1321 taaggctacc ataaaaaaaa aaatacatat atatataaat aaatatatta tattatataa 1381 tatatatatt aatgtttttg ttttttaata tttttttttt tttttgtaaa atatattatt 1441 tttggactta taaataattg tgtacataca tatattattt tattataata aatataataa 1501 tactataata atctttatgc aatatttgta attcatatat aataattttt aaaattatct 1561 atcttttgaa aaaaaaaaaa aaataaaaat aataaaaatt ataatgttat taaatactat 1621 aattattatg attaatataa tatatatata tatatatata tatatatata tatatatata 1681 ttttatatca tttaatatat tatgtgtgat ataataataa atccattgtc cataaataga 1741 tgaaatagcg gccgcctacc ctgaagaaga aaagtccgat gatgttgtgg acataaagaa 1801 ttgagctgtc tcaaaaaatg agaaaacaaa caaacaaaca aaataccatt atgcgcatat 1861 cctctgctca atatcaattt ttctttatgc cagttttgtt tcgacactat ttgagcaaca 1921 ccttcccttt actatctact actgttacaa tgttatttta ttcacctttt tacaatttta 1981 tcaaaatttt tttgtatgca tataaattat acattgtaaa gccaaagtgt agataaagac 2041 accttgttta tttttcccca attttttttt tttaccattt ttttattaac cttgctcatt 2101 tacctgctaa tacgattgct atatgatatc gaaattgaag gaaaaaccat catttgtgtt 2161 tatatgtttt tttttaattt ttcaactttt tttatttaac tatgctaatc atattataaa 2221 cattttaaaa atcaagtcca actatttatg aatcattgaa ggagacacat aattataaca 2281 gtaaaaataa taataataat aataattttg tcttattgtt tcaaacatat tatgttccat 2341 aaatatctaa atttttaata tggtcataaa ttcataggat ttaatttata taaaatatgg 2401 ctattcatac tagccatttt atgtgtgtaa agtttttttt ttctgaacaa aaaataaaat 2461 gttcataatt ttagctattt acatgcatgt gcatgcacaa aaaaaaatat gcacacaaca 2521 tacacatttt tacagttata aatacaatca attggtataa atatataaat aagaaaaacg 2581 aacattaagc tgccatatcc ctcgagttac ttgtcgtcgt cgtccttgta gtccactttc 2641 cgctttttct taggatcttc caccttgcgt tttttcttgg ggtcgcctcc cagctgagac 2701 aggtcgatcc gtgtctcgta caggccggtg atgctctggt ggatcagggt ggcgtccagc 2761 acctctttgg tgctggtgta cctcttccgg tcgatggtgg tgtcaaagta cttgaaggcg 2821 gcaggggctc ccagattggt cagggtaaac aggtggatga tattctcggc ctgctctctg 2881 ataggcttgt ctctgtgctt gttgtaggcg ctcagcacct tgtccagatt agcgtcggcc 2941 aggatcactc tcttggagaa ctcgctgatc tgctcgatga tctcgtccag gtagtgtttg 3001 tgctgttcca caaacagctg tttctgctca ttatcctcgg gggagccctt cagcttctca 3061 tagtggctgg ccaggtacag gaagttcaca tatttggagg gcagggccag ttcgtttccc 3121 ttctgcagtt cgccggcaga ggccagcatt ctcttccggc cgttttccag ctcgaacagg 3181 gagtacttag gcagcttgat gatcaggtcc tttttcactt ctttgtagcc cttggcttcc 3241 agaaagtcga tgggattctt ctcgaagctg cttctttcca tgatggtgat ccccagcagc 3301 tctttcacac tcttcagttt cttggacttg cccttttcca ctttggccac caccagcaca 3361 gaataggcca cggtggggct gtcgaagccg ccgtacttct tagggtccca gtccttcttt 3421 ctggcgatca gcttgtcgct gttcctcttg ggcaggatag actctttgct gaagccgcct 3481 gtctgcacct cggtcttttt cacgatattc acttggggca tagacagcac tttccgcacg 3541 gtggcaaagt cccggccctt atcccacacg atctcgcctg tttcgccgtt tgtctcgatc 3601 agaggccgct tccggatctc gccgttggcc agggtaatct cggtcttgaa aaagttcatg 3661 atgttgctgt agaagaagta cttggcggta gccttgccga tttcctgctc gctcttggcg 3721 atcatcttcc gcacgtcgta caccttgtag tcgccgtaca cgaactcgct ttccagctta 3781 gggtactttt tgatcagggc ggttcccacg acggcgttca ggtaggcgtc gtgggcgtgg 3841 tggtagttgt tgatctcgcg cactttgtaa aactggaaat ccttccggaa atcggacacc 3901 agcttggact tcagggtgat cactttcact tcccggatca gtttgtcgtt ctcgtcgtac 3961 ttagtgttca tccgggagtc caggatctgt gccacgtgct ttgtgatctg ccgggtttcc 4021 accagctgtc tcttgatgaa gccggcctta tccagttcgc tcaggccgcc tctctcggcc 4081 ttggtcagat tgtcgaactt cctctgggta atcagcttgg cattcagcag ctggcgccag 4141 tagttcttca tcttcttcac gacctcttcg gagggcacgt tgtcgctctt gccccggttc 4201 ttgtcgctcc gagtcagcac tttgttatcg atggagtcgt ccttcagaaa gctctgaggc 4261 acgatatggt ccacatcgta gtcggacagc cggttgatgt ccagttcctg gtccacgtac 4321 atatcccgcc cattctgcag gtagtacagg tacagcttct cgttctgcag ctgggtgttt 4381 tccacggggt gttctttcag gatctggctg cccagctctt tgatgccctc ttcgatccgc 4441 ttcattctct cgcggctgtt cttctgtccc ttctgggtgg tctggttctc tctggccatt 4501 tcgatcacga tgttctcggg cttgtgccgg cccatcactt tcacgagctc gtccaccacc 4561 ttcactgtct gcaggatgcc cttcttaatg gcggggctgc cggccagatt ggcaatgtgc 4621 tcgtgcaggc tatcgccctg gccggacacc tgggctttct ggatgtcctc tttaaaggtc 4681 aggctgtcgt cgtggatcag ctgcatgaag tttctgttgg cgaagccgtc ggacttcagg 4741 aaatccagga ttgtcttgcc ggactgcttg tcccggatgc cgttgatcag cttccggctc 4801 agcctgcccc agccggtgta tctccgccgc ttcagctgct tcatcacttt gtcgtcgaac 4861 aggtgggcat aggttttcag ccgttcctcg atcatctctc tgtcctcaaa cagtgtcagg 4921 gtcagcacga tatcttccag aatgtcctcg ttttcctcat tgtccaggaa gtccttgtcc 4981 ttgataattt tcagcagatc gtggtatgtg cccagggagg cgttgaaccg atcttccacg 5041 ccggagattt ccacggagtc gaagcactcg attttcttga agtagtcctc tttcagctgc 5101 ttcacggtca ctttccggtt ggtcttgaac agcaggtcca cgatggcttt tttctgctcg 5161 ccgctcagga aggcgggctt tctcattccc tcggtcacgt atttcacttt ggtcagctcg 5221 ttgtacacgg tgaagtactc gtacagcagg ctgtgcttgg gcagcacctt ctcgttgggc 5281 aggttcttat cgaagttggt catccgctcg atgaagctct gggcgctggc gcccttgtcc 5341 accacttcct cgaagttcca gggggtgatg gtttcctcgc tctttctggt catccaggcg 5401 aatctgctgt ttcccctggc cagagggccc acgtagtagg ggatgcggaa ggtcaggatc 5461 ttctcgatct tttcccggtt gtccttcagg aatgggtaaa aatcttcctg ccgccgcaga 5521 atggcgtgca gctctcccag gtggatctgg tgggggatgc tgccgttgtc gaaggtccgc 5581 tgcttccgca gcaggtcctc tctgttcagc ttcacgagca gttcctcggt gccgtccatc 5641 ttttccagga tgggcttgat gaacttgtag aactcttcct ggctggctcc gccatcgatg 5701 tagccggcgt agccgttctt gctctggtcg aagaaaatct ctttgtactt ctcaggcagc 5761 tgctgccgca cgagagcttt cagcagggtc aggtcctggt ggtgctcgtc gtatctcttg 5821 atcatagagg cgctcagggg ggccttggtg atctcggtgt tcactctcag gatgtcgctc 5881 agcaggatgg cgtcggacag gttcttggcg gccagaaaca ggtcggcgta ctggtcgccg 5941 atctgggcca gcaggttgtc caggtcgtcg tcgtaggtgt ccttgctcag ctgcagtttg 6001 gcatcctcgg ccaggtcgaa gttgctcttg aagttggggg tcaggcccag gctcagggca 6061 atcaggttgc cgaacaggcc attcttcttc tcgccgggca gctgggcgat cagattttcc 6121 agccgtctgc tcttgctcag tctggcagac aggatggcct tggcgtccac gccgctggcg 6181 ttgatggggt tttcctcgaa cagctggttg taggtctgca ccagctggat gaacagcttg 6241 tccacgtcgc tgttgtcggg gttcaggtcg ccctcgatca ggaagtggcc ccggaacttg 6301 atcatgtggg ccagggccag atagatcagc cgcaggtcgg ccttgtcggt gctgtccacc 6361 agtttctttc tcaggtggta gatggtgggg tacttctcgt ggtaggccac ctcgtccacg 6421 atgttgccga agatggggtg ccgctcgtgc ttcttatcct cttccaccag gaaggactct 6481 tccagtctgt ggaagaagct gtcgtccacc ttggccatct cgttgctgaa gatctcttgc 6541 agatagcaga tccggttctt ccgtctggtg tatcttcttc tggcggttct cttcagccgg 6601 gtggcctcgg ctgtttctcc gctgtcgaac agcagggcgc cgatcaggtt cttcttgatg 6661 ctgtgccggt cggtgttgcc cagcaccttg aatttcttgc tgggcacctt gtactcgtcg 6721 gtgatcacgg cccagcccac agagttggtg ccgatgtcca ggccgatgct gtacttcttg 6781 tccatttcga aggatcctga tatatttcta ttaggtattt attattataa aatataaatc 6841 ttgaatgata ataaataaaa tattagttat tccttttcta gtttaaaata tacatattat 6901 aaatatatat atatatatat atatttttat tgtgacaaga atatataatt ataaattata 6961 ttatttattt ttgtattttt tttttttttt tttttttttt ctttttttgt tttatttttc 7021 ttttttttta taaatattat ttttttcttt tatcatgcac attggaataa tacattaata 7081 tatatatata tattatatta tacatatatt gaataatgtt tataaaaaat gcataactta 7141 tatgaatata atttttttta aatatgacaa aaagaaaaaa aaaaaaaacc aaaaaaaatt 7201 aaaattgaaa tgaaatatat aaatatatta tttatatata ttatacattg tttaatacta 7261 ctacatgtat atatatatat tatatatata tatatatatc aattttttca aaaataaatt 7321 aatataaaaa gaggggaaaa aaaaaaaaaa aaaaaaaaaa agataattaa gtaagcattt 7381 aaaaatatat aaattgataa tatataaaat taatcacata taaactaata taatttataa 7441 aataaggaaa ataaaatatt accataaaat aaaaataaaa ataaaaaaaa aaaaaaaaaa 7501 cacctttttt tatatatatt aatatataat tatctcttag aaaaaatatt gtataattat 7561 atatgtaatg atttatataa aaaaataaaa ttatacaagt atatattttg tttctataaa 7621 ttgatatctt aattatttat tattagaaat agatattttt ataataaacc aatagataaa 7681 atttgtagag aaaaaaaaat aaaaataaaa ataaaaataa tataatatat aataaaataa 7741 aataatatta tataaatata ttttaatttt ttttacaaaa tgtctagaat tcatatcgat 7801 aactccatgg aactcctagg ctggcgcctg atgcggtatt ttctccttac gcatctgtgc 7861 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 7921 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 7981 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 8041 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt 8101 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 8161 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 8221 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 8281 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 8341 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 8401 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 8461 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 8521 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 8581 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 8641 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 8701 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 8761 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatgccaaca 8821 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 8881 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 8941 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 9001 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 9061 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 9121 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 9181 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 9241 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 9301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 9361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 9421 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 9481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 9541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 9601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 9661 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 9721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 9781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 9841 cgatttttgt gatgctcgtc aggggggcgg agcctatcga aaaacgccag caacgcggcc 9901 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 9961 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 10021 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 10081 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 10141 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 10201 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 10261 tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga 10321 atactcaagc t //