LOCUS Exported 12974 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of human MEFV M680I after doxycycline administration. ACCESSION . VERSION . KEYWORDS p21-M680I-MEFV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12974) AUTHORS Magnotti F, Lefeuvre L, Benezech S, Malsot T, Waeckel L, Martin A, Kerever S, Chirita D, Desjonqueres M, Duquesne A, Gerfaud-Valentin M, Laurent A, Seve P, Popoff MR, Walzer T, Belot A, Jamilloux Y, Henry T TITLE Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. JOURNAL EMBO Mol Med. 2019 Oct 7:e10547. doi: 10.15252/emmm.201910547. PUBMED 31589380 REFERENCE 2 (bases 1 to 12974) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12974 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2230..2500 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2533..2571 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2568..2592 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 2701..2725 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2768..2833 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" protein_bind complement(5203..5227) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5280..5306 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" promoter 5526..6704 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 5757..6695 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 6652..6672 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind complement(6774..6807) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 6814..7560 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" misc_feature 7613..8186 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7780..7797) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 8007..8026 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8202..8921 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 8202..8918 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8247..8268) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8508..8527) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8855..8876 /label=EGFP-C /note="EGFP, forward primer" primer_bind 9057..9076 /label=Neo-F /note="Neomycin resistance gene, forward primer" misc_feature 9364..9952 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9417..9437) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9835..9846) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 10027..10260 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(10379..10397) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10465..10569 /gene="bla" /label=AmpR promoter CDS 10570..11430 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10788..10807) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11601..12189 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 12090..12109 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12343..12360 /label=L4440 /note="L4440 vector, forward primer" primer_bind 12452..12471 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 12600..12621 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12636..12666 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12674..12690 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12679..12701 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12698..12714 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12698..12714 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12764..12783 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc 2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca 2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa 2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa 2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg 2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg 2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca 2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac 2761 caccatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga 2821 tgacgatgac aagcttgcgg ccgcggctaa gacccctagt gaccatctgc tgtccaccct 2881 ggaggagctg gtgccctatg acttcgagaa gttcaagttc aagctgcaga acaccagtgt 2941 gcagaaggag cactccagga tcccccggag ccagatccag agagccaggc cggtgaagat 3001 ggccactctg ctggtcacct actatgggga agagtacgcc gtgcagctca ccctgcaggt 3061 cctgcgggcc atcaaccagc gcctgctggc cgaggagctc cacagggcag ccattcagga 3121 atattccaca caagaaaacg gcacagatga ttccgcagcg tccagctccc tgggggagaa 3181 caagcccagg agcctgaaga ctccagacca ccccgagggg aacgagggga acggccctcg 3241 gccgtacggg ggcggagctg ccagcctgcg gtgcagccag cccgaggccg ggagggggct 3301 gtcgaggaag cccctgagca aacgcagaga gaaggcctcg gagggcctgg acgcgcaggg 3361 caagcctcgg acccggagcc cggccctgcc gggcgggaga agccccggcc cctgcagggc 3421 gctagagggg ggccaggccg aggtccggct gcgcagaaac gccagctccg cggggaggct 3481 gcaggggctg gcggggggcg ccccggggca gaaggagtgc aggcccttcg aagtgtacct 3541 gccctcggga aagatgcgac ctagaagcct tgaggtcacc atttctacag gggagaaggc 3601 gcccgcaaat ccagaaattc tcctgactct agaggaaaag acagctgcga atctggactc 3661 ggcaacagaa ccccgggcaa ggcccactcc ggatggaggg gcatctgcgg acctgaagga 3721 aggccctgga aatccagaac attcggtcac cggaaggcca ccagacacgg ctgcgagtcc 3781 ccgctgccac gcccaggaag gagacccagt tgacggtacc tgtgtgcgtg attcctgcag 3841 cttccccgag gcagtttctg ggcaccccca ggcctcaggc agccgctcac ctggctgccc 3901 ccggtgccag gactcccatg aaaggaagag cccgggaagc ctaagccccc agcccctgcc 3961 acagtgtaag cgccacctga agcaggtcca gctgctcttc tgtgaggatc acgatgagcc 4021 catctgcctc atctgcagtc tgagtcagga gcaccaaggc caccgggtgc gccccattga 4081 ggaggtcgcc ctggaacaca agaagaaaat tcagaagcag ctggagcatc tgaagaagct 4141 gagaaaatca ggggaggagc agcgatccta tggggaggag aaggcagtga gctttctgaa 4201 acaaactgaa gcgctgaagc agcgggtgca gaggaagctg gagcaggtgt actacttcct 4261 ggagcagcaa gagcatttct ttgtggcctc actggaggac gtgggccaga tggttgggca 4321 gatcaggaag gcatatgaca cccgcgtatc ccaggacatc gccctgctcg atgcgctgat 4381 tggggaactg gaggccaagg agtgccagtc agaatgggaa cttctgcagg acattggaga 4441 catcttgcac agggctaaga cagtgcctgt ccctgaaaag tggaccactc ctcaagagat 4501 aaaacaaaag atccaactcc tccaccagaa gtcagagttt gtggagaaga gcacaaagta 4561 cttctcagaa accctgcgtt cagaaatgga aatgttcaat gttccagagc tgattggcgc 4621 tcaggcacat gctgttaatg tgattctgga tgcagaaacc gcttacccca acctcatctt 4681 ctctgatgat ctgaagagtg ttagacttgg aaacaagtgg gagaggctgc ctgatggccc 4741 gcaaagattt gacagctgta tcattgttct gggctctccg agtttcctct ctggccgccg 4801 ttactgggag gtggaggttg gagacaagac agcatggatc ctgggagcct gcaagacatc 4861 cataagcagg aaagggaaca taactctgtc gccagagaat ggctactggg tggtgataat 4921 gatgaaggaa aatgagtacc aggcgtccag cgttcccccg acccgcctgc taataaagga 4981 gcctcccaag cgtgtgggca tcttcgtgga ctacagagtt ggaagcatct ccttttacaa 5041 tgtgacagcc agatcccaca tctatacatt cgccagctgc tctttctctg ggccccttca 5101 acctatcttc agccctggga cacgtgatgg agggaagaac acagctcctc tgactatctg 5161 tccagtgggt ggtcaggggc ctgactgact cgagatatct agacccagct ttcttgtaca 5221 aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaat 5281 acccatacga cgtcccagac tacgcttagt aatgattaat taaactagaa attctaccgg 5341 gtaggggagg cgcttttccc aaggcagtct ggagtaaccc acccaagatc tggcctccgc 5401 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagccgc gtaagatctg 5461 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcctagt 5521 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 5581 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 5641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 5701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 5761 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 5821 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg 5881 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg 5941 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg 6001 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt 6061 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg 6121 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc 6181 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg 6241 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg 6301 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat 6361 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct 6421 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc 6481 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg 6541 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga 6601 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc 6661 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagcggc cgccatgcta 6721 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgga tccataactt 6781 cgtataatgt atgctatacg aagttatgcc accatgtcta gactggacaa gagcaaagtc 6841 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa 6901 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg 6961 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc 7021 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct 7081 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag 7141 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac 7201 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag 7261 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt 7321 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc 7381 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc 7441 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac 7501 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa 7561 ctaagtaagg atccgcggcc gcactagagg aattccgccc ctctcccccc cccccctctc 7621 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 7681 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 7741 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 7801 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 7861 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 7921 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 7981 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 8041 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 8101 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg 8161 ttttcctttg aaaaacacga tgataagctt accggtccac catggtgagc aagggcgagg 8221 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca 8281 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt 8341 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct 8401 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt 8461 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact 8521 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga 8581 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca 8641 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca 8701 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca 8761 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg 8821 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg 8881 ccgccgggat cactctcggc atggacgagc tgtacaagta agcatgcccg acggcgagga 8941 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 9001 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 9061 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 9121 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 9181 cttctgatgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg 9241 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 9301 tgaatgcaat tgttgttgtt tagtccctcc caattcgata tcaagcttat cgcgatagat 9361 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 9421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 9481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 9541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 9601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 9661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 9721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 9781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 9841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 9901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc 9961 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 10021 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 10081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 10141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 10201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 10261 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 10321 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc 10381 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 10441 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 10501 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 10561 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 10621 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 10681 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 10741 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 10801 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 10861 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 10921 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 10981 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 11041 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 11101 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 11161 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 11221 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 11281 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 11341 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 11401 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 11461 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 11521 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 11581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 11641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 11701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 11761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 11821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 11881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 11941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 12001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 12061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 12121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 12181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 12241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 12301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 12361 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 12421 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 12481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 12541 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 12601 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 12661 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt 12721 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 12781 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 12841 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 12901 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 12961 acaaacttag tagt //