LOCUS       Exported               12973 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of human MEFV P369S after doxycycline 
            administration.
ACCESSION   .
VERSION     .
KEYWORDS    p21-P369S-MEFV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12973)
  AUTHORS   Magnotti F, Lefeuvre L, Benezech S, Malsot T, Waeckel L, Martin A, 
            Kerever S, Chirita D, Desjonqueres M, Duquesne A, Gerfaud-Valentin 
            M, Laurent A, Seve P, Popoff MR, Walzer T, Belot A, Jamilloux Y, 
            Henry T
  TITLE     Pyrin dephosphorylation is sufficient to trigger inflammasome 
            activation in familial Mediterranean fever patients.
  JOURNAL   EMBO Mol Med. 2019 Oct 7:e10547. doi: 10.15252/emmm.201910547.
  PUBMED    31589380
REFERENCE   2  (bases 1 to 12973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2500
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2768..2833
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5203..5227)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5280..5306
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5526..6704
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5757..6695
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6652..6672
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(6774..6807)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             6814..7560
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7613..8185
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7779..7796)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8006..8025
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8201..8920
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8201..8917
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8246..8267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8507..8526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8854..8875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9056..9075
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9363..9951
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9416..9436)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9834..9845)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10026..10259
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10378..10396)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10464..10568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10569..11429
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10787..10806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11600..12188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12089..12108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12342..12359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12451..12470
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    12599..12620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12635..12665
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12673..12689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12678..12700
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12697..12713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12697..12713
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12763..12782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac
     2761 caccatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     2821 tgacgatgac aagcttgcgg ccgcggctaa gacccctagt gaccatctgc tgtccaccct
     2881 ggaggagctg gtgccctatg acttcgagaa gttcaagttc aagctgcaga acaccagtgt
     2941 gcagaaggag cactccagga tcccccggag ccagatccag agagccaggc cggtgaagat
     3001 ggccactctg ctggtcacct actatgggga agagtacgcc gtgcagctca ccctgcaggt
     3061 cctgcgggcc atcaaccagc gcctgctggc cgaggagctc cacagggcag ccattcagga
     3121 atattccaca caagaaaacg gcacagatga ttccgcagcg tccagctccc tgggggagaa
     3181 caagcccagg agcctgaaga ctccagacca ccccgagggg aacgagggga acggccctcg
     3241 gccgtacggg ggcggagctg ccagcctgcg gtgcagccag cccgaggccg ggagggggct
     3301 gtcgaggaag cccctgagca aacgcagaga gaaggcctcg gagggcctgg acgcgcaggg
     3361 caagcctcgg acccggagcc cggccctgcc gggcgggaga agccccggcc cctgcagggc
     3421 gctagagggg ggccaggccg aggtccggct gcgcagaaac gccagctccg cggggaggct
     3481 gcaggggctg gcggggggcg ccccggggca gaaggagtgc aggcccttcg aagtgtacct
     3541 gccctcggga aagatgcgac ctagaagcct tgaggtcacc atttctacag gggagaaggc
     3601 gcccgcaaat ccagaaattc tcctgactct agaggaaaag acagctgcga atctggactc
     3661 ggcaacagaa ccccgggcaa ggcccactcc ggatggaggg gcatctgcgg acctgaagga
     3721 aggccctgga aatccagaac attcggtcac cggaaggcca ccagacacgg ctgcgagtcc
     3781 ccgctgccac gcccaggaag gagacccagt tgacggtacc tgtgtgcgtg attcctgcag
     3841 cttccccgag gcagtttctg ggcaccccca ggcctcaggc agccgctcac ctggctgccc
     3901 ccggtgccag gactcccatg aaaggaagag cccgggaagc ctaagctccc agcccctgcc
     3961 acagtgtaag cgccacctga agcaggtcca gctgctcttc tgtgaggatc acgatgagcc
     4021 catctgcctc atctgcagtc tgagtcagga gcaccaaggc caccgggtgc gccccattga
     4081 ggaggtcgcc ctggaacaca agaagaaaat tcagaagcag ctggagcatc tgaagaagct
     4141 gagaaaatca ggggaggagc agcgatccta tggggaggag aaggcagtga gctttctgaa
     4201 acaaactgaa gcgctgaagc agcgggtgca gaggaagctg gagcaggtgt actacttcct
     4261 ggagcagcaa gagcatttct ttgtggcctc actggaggac gtgggccaga tggttgggca
     4321 gatcaggaag gcatatgaca cccgcgtatc ccaggacatc gccctgctcg atgcgctgat
     4381 tggggaactg gaggccaagg agtgccagtc agaatgggaa cttctgcagg acattggaga
     4441 catcttgcac agggctaaga cagtgcctgt ccctgaaaag tggaccactc ctcaagagat
     4501 aaaacaaaag atccaactcc tccaccagaa gtcagagttt gtggagaaga gcacaaagta
     4561 cttctcagaa accctgcgtt cagaaatgga aatgttcaat gttccagagc tgattggcgc
     4621 tcaggcacat gctgttaatg tgattctgga tgcagaaacc gcttacccca acctcatctt
     4681 ctctgatgat ctgaagagtg ttagacttgg aaacaagtgg gagaggctgc ctgatggccc
     4741 gcaaagattt gacagctgta tcattgttct gggctctccg agtttcctct ctggccgccg
     4801 ttactgggag gtggaggttg gagacaagac agcatggatc ctgggagcct gcaagacatc
     4861 cataagcagg aaagggaaca tgactctgtc gccagagaat ggctactggg tggtgataat
     4921 gatgaaggaa aatgagtacc aggcgtccag cgttcccccg acccgcctgc taataaagga
     4981 gcctcccaag cgtgtgggca tcttcgtgga ctacagagtt ggaagcatct ccttttacaa
     5041 tgtgacagcc agatcccaca tctatacatt cgccagctgc tctttctctg ggccccttca
     5101 acctatcttc agccctggga cacgtgatgg agggaagaac acagctcctc tgactatctg
     5161 tccagtgggt ggtcaggggc ctgactgact cgagatatct agacccagct ttcttgtaca
     5221 aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaat
     5281 acccatacga cgtcccagac tacgcttagt aatgattaat taaactagaa attctaccgg
     5341 gtaggggagg cgcttttccc aaggcagtct ggagtaaccc acccaagatc tggcctccgc
     5401 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagccgc gtaagatctg
     5461 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcctagt
     5521 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     5581 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     5641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     5701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     5761 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     5821 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     5881 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     5941 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     6001 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     6061 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     6121 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     6181 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     6241 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     6301 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     6361 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     6421 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     6481 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     6541 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     6601 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     6661 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagcggc cgccatgcta
     6721 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgga tccataactt
     6781 cgtataatgt atgctatacg aagttatgcc accatgtcta gactggacaa gagcaaagtc
     6841 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     6901 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     6961 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     7021 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     7081 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     7141 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     7201 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     7261 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     7321 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     7381 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     7441 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     7501 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     7561 ctaagtaagg atccgcggcc gcactagagg aattccgccc ctctcccccc cccccctctc
     7621 cctccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     7681 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     7741 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     7801 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     7861 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     7921 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     7981 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     8041 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     8101 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     8161 tttcctttga aaaacacgat gataagctta ccggtccacc atggtgagca agggcgagga
     8221 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     8281 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     8341 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     8401 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     8461 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     8521 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     8581 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     8641 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     8701 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     8761 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     8821 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     8881 cgccgggatc actctcggca tggacgagct gtacaagtaa gcatgcccga cggcgaggat
     8941 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     9001 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     9061 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     9121 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     9181 ttctgatgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     9241 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     9301 gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc gcgatagatc
     9361 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     9781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     9901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     9961 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10021 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
    10081 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10141 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10201 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10261 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    10321 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
    10381 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    10441 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10501 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10561 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10621 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10681 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10741 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10801 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10861 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10921 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10981 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11041 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11101 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11161 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11221 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11281 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11341 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11401 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11461 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11521 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12421 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    12481 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    12541 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    12601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    12661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    12721 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    12781 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    12841 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    12901 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    12961 caaacttagt agt
//
