LOCUS Exported 7140 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV BAF170-Flag SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7140) AUTHORS Xi Q, He W, Zhang XH, Le HV, Massague J TITLE Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the transforming growth factor beta transcriptional program. JOURNAL J Biol Chem. 2008 Jan 11. 283(2):1146-55. PUBMED 18003620 REFERENCE 2 (bases 1 to 7140) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7140 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 126..148 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 140..157 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 141..157 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 318..697 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 698..901 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 852..872 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 4582..4605 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind 4625..4646 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4763..4780) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4934..5522) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5014..5033) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5693..6553) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6316..6335 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6554..6658) /gene="bla" /label=AmpR promoter rep_origin 6685..7140 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6772..6791) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6982..7003 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attccccgag ccggagaaga tggcggtgcg gaagaaggac 961 ggcggcccca acgtgaagta ctacgaggcc gcggacaccg tgacccagtt cgacaacgtg 1021 cggctgtggc tcggcaagaa ctacaagaag tatatacaag ctgaaccacc caccaacaag 1081 tccctgtcta gcctggttgt acagttgcta caatttcagg aagaagtttt tggcaaacat 1141 gtcagcaatg caccgctcac taaactgccg atcaaatgtt tcctagattt caaagcggga 1201 ggctccttgt gccacattct tgcagctgcc tacaaattca agagtgacca gggatggcgg 1261 cgttacgatt tccagaatcc atcacgcatg gaccgcaatg tggaaatgtt tatgaccatt 1321 gagaagtcct tggtgcagaa taattgcctg tctcgaccta acatttttct gtgcccagaa 1381 attgagccca aactactagg gaaattaaag gacattatca agagacacca gggaacagtc 1441 actgaggata agaacaatgc ctcccatgtt gtgtatcctg tcccggggaa tctagaagaa 1501 gaggaatggg tacgaccagt catgaagagg gataagcagg ttcttctgca ctggggctac 1561 tatcctgaca gttacgacac gtggatccca gcgagtgaaa ttgaggcatc tgtggaagat 1621 gctccaactc ctgagaaacc taggaaggtt catgcaaagt ggatcctgga caccgacacc 1681 ttcaatgaat ggatgaatga ggaagactat gaagtaaatg atgacaaaaa ccctgtctcc 1741 cgccgaaaga agatttcagc caagacactg acagatgagg tgaacagccc agattcagat 1801 cgacgggaca agaagggggg aaactataag aagaggaagc gctccccctc tccttcacca 1861 accccagaag caaagaagaa aaatgctaag aaaggtccct caacacctta cactaagtca 1921 aagcgtggcc acagagaaga ggagcaagaa gacctgacaa aggacatgga cgagccctca 1981 ccagtcccca atgtagaaga ggtgacactt cccaaaacag tcaacacaaa gaaagactca 2041 gagtcggccc cagtcaaagg cggcaccatg accgacctgg atgaacagga agatgaaagc 2101 atggagacga cgggcaagga tgaggatgag aacagtacgg ggaacaaggg agagcagacc 2161 aagaatccag acctgcatga ggacaatgtg actgaacaga cccaccacat catcattccc 2221 agctacgctg cctggtttga ctacaatagt gttcatgcca ttgagcggag ggctctcccc 2281 gagttcttca acggcaagaa caagtccaag actccagaga tctacctggc ctatcgaaac 2341 tttatgattg acacttaccg actgaacccc caagagtatc ttacctctac cgcctgccgc 2401 cgaaacctag cgggtgatgt ctgtgccatc atgagggtcc atgccttcct agaacagtgg 2461 ggtcttatta actaccaggt ggatgctgag agtcgaccaa ccccaatggg gcctccgcct 2521 acctctcact tccatgtctt ggctgacaca ccatcagggc tggtgcctct gcagcccaag 2581 acacctcagc agacctctgc ttcccaacaa atgctcaact ttcctgacaa aggcaaagag 2641 aaaccaacag acatgcaaaa ctttgggctg cgcacagaca tgtacacaaa aaagaatgtt 2701 ccctccaaga gcaaggctgc agccagtgcc actcgtgagt ggacagaaca ggaaaccctg 2761 cttctcctgg aggcactgga aatgtacaaa gatgactgga acaaagtgtc cgagcatgtg 2821 ggaagccgca cacaggacga gtgcatcttg cattttcttc gtcttcccat tgaagaccca 2881 tacctggagg actcagaggc ctccctaggc cccctggcct accaacccat ccccttcagt 2941 cagtcgggca accctgttat gagcactgtt gccttcctgg cctctgtcgt cgatccccga 3001 gtcgcctctg ctgctgcaaa gtcagcccta gaggagttct ccaaaatgaa ggaagaggta 3061 cccacggcct tggtggaggc ccatgttcga aaagtggaag aagcagccaa agtaacaggc 3121 aaggcggacc ctgccttcgg tctggaaagc agtggcattg caggaaccac ctctgatgag 3181 cctgagcgga ttgaggagag cgggaatgac gaggctcggg tggaaggcca ggccacagat 3241 gagaagaagg agcccaagga accccgagaa ggagggggtg ctatagagga ggaagcaaaa 3301 gagaaaacca gcgaggctcc caagaaggat gaggagaaag ggaaagaagg cgacagtgag 3361 aaggagtccg agaagagtga tggagaccca atagtcgatc ctgagaagga gaaggagcca 3421 aaggaagggc aggaggaagt gctgaaggaa gtggtggagt ctgaggggga aaggaagaca 3481 aaggtggagc gggacattgg cgagggcaac ctctccaccg ctgctgccgc cgccctggcc 3541 gccgccgcag tgaaagctaa gcacttggct gctgttgagg aaaggaagat caaatctttg 3601 gtggccctgc tggtggagac ccagatgaaa aagttggaga tcaaacttcg gcactttgag 3661 gagctggaga ctatcatgga ccgggagcga gaagcactgg agtatcagag gcagcagctc 3721 ctggccgaca gacaagcctt ccacatggag cagctgaagt atgcggagat gagggctcgg 3781 cagcagcact tccaacagat gcaccaacag cagcagcagc caccaccagc cctgccccca 3841 ggctcccagc ctatcccccc aacaggggct gctgggccac ccgcagtcca tggcttggct 3901 gtggctccag cctctgtagt ccctgctcct gctggcagtg gggcccctcc aggaagtttg 3961 ggcccttctg aacagattgg gcaggcaggg tcaactgcag ggccacagca gcagcaacca 4021 gctggagccc cccagcctgg ggcagtccca ccaggggttc ccccccctgg accccatggc 4081 ccctcaccgt tccccaacca acaaactcct ccctcaatga tgccaggggc agtgccaggc 4141 agcgggcacc caggcgtggc gggtaatgct cctttgggtt tgccttttgg catgccgcct 4201 cctcctcctc ctcctgctcc atccatcatc ccatttggta gtctagctga ctccatcagt 4261 attaacctcc ccgctcctcc taacctgcat gggcatcacc accatctccc gttcgccccg 4321 ggcactctcc ccccacctaa cctgcctgtg tccatggcga accctctaca tcctaacctg 4381 ccggcgacca ccaccatgcc atcttccttg cctctcgggc cggggctcgg atccgccgca 4441 gcccaaagcc ctgccattgt ggcagctgtt cagggcaacc tcctgcccag tgccagccca 4501 ctgccagacc caggcacccc cctgcctcca gaccccacag ccccgagccc aggcacggtc 4561 acccctgtgc cacctccaca ggactacaag gacgacgatg acaagtgaaa gcttggggtg 4621 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 4681 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 4741 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 4801 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 4861 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 4921 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 4981 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 5041 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 5101 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 5161 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 5221 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 5281 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 5341 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 5401 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 5461 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 5521 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 5581 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 5641 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 5701 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 5761 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 5821 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 5881 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 5941 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 6001 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 6061 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 6121 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 6181 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 6241 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 6301 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 6361 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 6421 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 6481 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 6541 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 6601 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 6661 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg 6721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 6781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 6841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 6901 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 6961 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 7021 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 7081 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt //