LOCUS       Exported               14339 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plant expression vector for direct cloning of synthetic trans-acting
            siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+].
            Contains AtMIR173 for syntasi expression in any plant species..
ACCESSION   .
VERSION     .
KEYWORDS    pMDC32B-AtTAS1c-D2-B/c-AtMIR173
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Lopez-Dolz L, Spada M, Daros JA, Carbonell A
  TITLE     Fine-tune control of targeted RNAi efficacy by plant artificial 
            small RNAs.
  JOURNAL   Nucleic Acids Res. 2020 May 12. pii: 5836195. doi: 
            10.1093/nar/gkaa343.
  PUBMED    32396204
REFERENCE   2  (bases 1 to 14339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3891)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(4325..4954)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6254..6278
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6466..6488
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6480..6497
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6481..6497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6924..7268
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7803..7833
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7887..8546
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7953..7972)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8888..9193
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9072..9091
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9848..9872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        10615..10959
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    11019..11043
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11589..11613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11948..11964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11948..11964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11961..11983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11972..11988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11996..12026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12041..12062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12253..12930
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             12998..14023
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    14063..14237
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14315..14339
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaagagaact tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccccttcacc
     7381 aaacctaaac ctaaacggct aagcccgacg tcaaatacca aaaagagaaa aacaagagcg
     7441 ccgtcaagct ctgcaaatac gatctgtaag tccatcttaa cacaaaagtg agatgggttc
     7501 ttagatcatg ttccgccgtt agatcgagtc atggtcttgt ctcatagaaa ggtactttcg
     7561 tttacttctt ttgagtatcg agtagagcgt cgtctatagt tagtttgaga ttgcgtttgt
     7621 cagaagttag gttcaatgtc ccggtccaat tttcaccagc catgtgtcag tttcgttcct
     7681 tcccgtcctc ttctttgatt tcgttgggtt acggatgttt tcgagatgaa acagcattgt
     7741 tttgttgtga tttttctcta caagcgaata gaccatttaa gagaccatta ggcaccccag
     7801 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gagccgtcga
     7861 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     7921 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     7981 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     8041 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     8101 agttccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     8161 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     8221 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     8281 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     8341 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     8401 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     8461 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     8521 gcgatgagtg gcagggcggg gcgtaaacgc gtggagccgg cttactaaaa gccagataac
     8581 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     8641 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     8701 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     8761 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     8821 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     8881 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     8941 ggatgtacag agtgatatta ttgacacgcc cggccgacgg atggtgatcc ccctggccag
     9001 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     9061 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg ttatcgggga
     9121 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     9181 ctggggaata taaatgtcag gctcccttat acacagccag tctgcacctc gacggtctca
     9241 tcggtggatc ttagaaaatt attctaagtc caacatagcg tattctaagt tcaacatatc
     9301 gacgaactag aaaagacatt ggacatattc caggatatgc aaaagaaaac aatgaatatt
     9361 gttttgaatg tgttcaagta aatgagattt tcaagtcgtc taaagaacag ttgctaatac
     9421 agttacttat ttcaataaat aattggttct aataatacaa aacatattcg aggatatgca
     9481 gaaaaaaaga tgtttgttat tttgaaaagc ttgagtagtt tctctccgag gtgtagcgaa
     9541 gaagcatcat ctactttgta atgtaatttt ctttatgttt tcactttgta attttatttg
     9601 tgttaatgta ccatggccga tatcggtttt attgaaagaa aatttatgtt acttctgttt
     9661 tggctttgca atcagttatg ctagttttct tatacccttt cgtaagcttc ctaaggaatc
     9721 gttcattgat ttccactgct tcattgtata ttaaaacttt acaactgtat cgaccatcat
     9781 ataattctgg gtcaagagat gaaaatagaa caccacatcg taaagtgaaa taagggtggg
     9841 cgcgccgacc cagctttctt gtacaaagtg gttcgataat tccttaatta actagttcta
     9901 gagcggccgc ccaccgcggt ggagctcgaa tttccccgat cgttcaaaca tttggcaata
     9961 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
    10021 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
    10081 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
    10141 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactgaatt caagcttggc
    10201 gtgcctgcag gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga
    10261 tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
    10321 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
    10381 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
    10441 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
    10501 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac
    10561 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
    10621 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
    10681 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
    10741 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
    10801 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
    10861 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
    10921 ttcctctata taaggaagtt catttcattt ggagaggacc tcgactctag aggatccccg
    10981 ggtaccgggc cccccctcga ggcgcgccaa gctatcaaac aagtttgtac aaaaaagcag
    11041 gctccgcggc cgcccgcttc accataatta gcaagtaata aggaataaaa tcctgtaatt
    11101 atagctgaaa aaggaaaaat atttggagtc cgtcagattc gaatctgaac aaagcataaa
    11161 aaagtcaaca aaacttaaag cggcggactc atcgtaatct cagcccaata ccctattttc
    11221 ctctccccta tataaatact ttcttcttct actgatcttc ttctcacaaa taaacccaaa
    11281 tatatcaatc tactgtgttg gtgattaagt actttcgctt gcagagagaa atcacagtgg
    11341 tcaaaaaagt tgtagttttc ttaaagtctc tttcctctgt gattctctgt gtaagcgaaa
    11401 gagcttgctc cctaaactta tctctctgat gatttaatgt tagagatctt cgtaaatcta
    11461 tgtgtttgat agatctgatg cgttttttga gttgatgatt tgattatttt tcactggaaa
    11521 gtatctcatt agggtaacga taatgtttta tggatttggt tgtataacag ataagggtgg
    11581 gcgcgccgac ccagctttct tgtacaaagt ggttcgataa ttccttaatt aactagttct
    11641 agagcggccg cccaccgcgg tggagctcga atttccccga tcgttcaaac atttggcaat
    11701 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
    11761 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
    11821 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
    11881 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactgaat tcgaattcgt
    11941 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    12001 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    12061 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    12121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa
    12181 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
    12241 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
    12301 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
    12361 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
    12421 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
    12481 ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacactctcg tctactccaa
    12541 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
    12601 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
    12661 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
    12721 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
    12781 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
    12841 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gaccttcctc tatataagga
    12901 agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac aaatctatct
    12961 ctctcgagct ttcgcagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc
    13021 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
    13081 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
    13141 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
    13201 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
    13261 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
    13321 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
    13381 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
    13441 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
    13501 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
    13561 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
    13621 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
    13681 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
    13741 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
    13801 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
    13861 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
    13921 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
    13981 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccgga
    14041 tctgtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
    14101 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
    14161 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
    14221 gtactaaaat ccagatcccc cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc
    14281 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgcca
//
