LOCUS Exported 8684 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles. ACCESSION . VERSION . KEYWORDS pLenti-Tet SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8684) AUTHORS Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM TITLE Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis. JOURNAL EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3. PUBMED 31268602 REFERENCE 2 (bases 1 to 8684) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8684 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2649..2667 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2686..2704 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2723..2741 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2760..2778 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2797..2815 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2834..2852 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2871..2889 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2908..2926 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 2948..2972 /label=LNCX /note="Human CMV promoter, forward primer" promoter 3161..3372 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" regulatory 3380..3389 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3386..3760 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" misc_feature 3776..4364 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3829..3849) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4247..4258) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4436..4669 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(4695..4712) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4701..4925 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4971..5399 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5058..5077) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5268..5289 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5408..5428) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5413..5742 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5593..5728 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5655..5674 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 5790..5837 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 5856..6230 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6360..6481 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6397..6416) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6451..6470 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6530..6546) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6530..6546) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6543..6565) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6554..6570 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6578..6608) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6623..6644 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6761..6778) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6932..7520) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7012..7031) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7691..8551) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8314..8333 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8552..8656) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccggg agtatccgct cgagtccgga tctcgacccg 2641 ggcacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata 2701 gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct 2761 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg 2821 acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag 2881 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt accccctata 2941 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 3001 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattt gctaggaatt 3061 agcgctaccg gtgcggccgc accatggatc cctgatcgat cggccgaatt cgctagctag 3121 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc 3181 acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg 3241 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3301 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3361 gccagaacac aggaccggtg ccaccatggc caagttgacc agtgccgttc cggtgctcac 3421 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga 3481 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc 3541 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga 3601 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc 3661 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc 3721 cggcaactgc gtgcacttcg tggccgagga gcaggactga acgcgttaag tcgacaatca 3781 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 3841 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 3901 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 3961 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 4021 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 4081 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 4141 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 4201 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 4261 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 4321 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca 4381 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 4441 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 4501 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 4561 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 4621 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta 4681 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 4741 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 4801 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 4861 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 4921 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 4981 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 5041 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 5101 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 5161 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 5221 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 5281 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 5341 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 5401 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 5461 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 5521 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 5581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 5641 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 5701 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 5761 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg 5821 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc 5881 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 5941 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 6001 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 6061 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 6121 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 6181 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac 6241 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 6301 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 6361 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6421 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6481 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 6541 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6601 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 6661 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6721 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6781 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6841 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6901 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6961 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 7021 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 7081 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 7141 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 7201 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7261 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7321 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 7381 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7441 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7501 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7561 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7621 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7681 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7741 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7801 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7861 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7921 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7981 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 8041 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 8101 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 8161 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 8221 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8281 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8341 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8401 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8461 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8521 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8581 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8641 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac //