LOCUS       Exported               13805 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces.
ACCESSION   .
VERSION     .
KEYWORDS    pSABEn
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Zhong Z, Guo J, Deng L, Chen L, Wang J, Li S, Xu W, Deng Z, Sun Y
  TITLE     Base editing in Streptomyces with Cas9-deaminase fusions
  JOURNAL   BioRxiv 630137
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(250..270)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(250..268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(297..315)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        383..487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             488..1348
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(706..725)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1519..2107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2008..2027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2261..2278
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2492..2626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2516..2535)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2570..2589
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5844..6145
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
     CDS             7337..11440
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     oriT            11803..11912
                     /note="incP origin of transfer"
     CDS             11945..12316
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_RNA        12472..12547
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(13061..56)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
ORIGIN
        1 agctcggctt ttcgccattc gtattgcacg acattgcact ccaccgctga tgacatcagt
       61 cgatcatagc acgatcaacg gcactgttgc aaatagtcgg tggtgataaa cttatcatcc
      121 ccttttgctg atggagctgc acatgaaccc attcaaaggc cggcattttc agcgtgacat
      181 cattctgtgg gccgtacgct ggtactgcaa atacggcatc agttaccgtg agctgcaggt
      241 cgacggatcc ctttagtgag ggttaattgc ggccgcgaat tcttgaagac gaaagggcct
      301 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      361 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      421 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      481 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      541 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      601 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      661 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      721 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      781 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      841 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      901 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      961 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1021 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1081 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1141 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1201 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1261 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1321 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1381 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1441 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1621 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     1681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2161 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2221 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2281 agcggaagag cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     2341 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     2401 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     2461 acgacaggag cacgatcatg cgcacccgtc agatccagac atgataagat acattgatga
     2521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcccg cacgatcagc
     2641 gtcccgccga ccaggagcag tgcggccagc actgccgcta acgcctggtc ctggtcccgg
     2701 tcctggtggt gcatcagtcc tccccgtgat cacttcggca cccaccgtag tgatcacccc
     2761 cgacagcgga tcaaggggtt tgcgggtccc ggtcggcgcc gggcggggga ggcaggagcc
     2821 gccgacgctg cctctgggac gggccggacg gcagggggac cggcggccgg gcgagctgca
     2881 gccgggggtc cggcagggcc ggagcgggcg gaaccgtgct ctgacctgcg gcccgagttt
     2941 cgtcacgtga cggaatggaa ggctgctgca tttcgtcacg tgacgtatct cggcgagcga
     3001 ctgccgacgc cacggcggac acgatcgcct cgcgctggcg ccgggcctcg tacgcccgct
     3061 ggcggcagga gcggcggcag tagtcccggc tccggccgac gccggattgc ttgatctccg
     3121 agccgcacca ggcgcagagc ttcgcgccgt cggcgtccct gggggtggtg gtgctcatgg
     3181 ccgacgaccg tacgcggcac gtctcgtagc gaggcgagtc gggcgcgagg taccgcctgc
     3241 acgaagtgcc ggcggggccg accccgggcg agtaatccca ggattactcc cgcggcttcg
     3301 accccggccg ccgtcgccgc gtacgtcacc gacccccgcc gtacgtcacc gggatgacgt
     3361 acggcggggg ggagcgagtt agtgcgaagt gggcccactt gcgagccggg cgatgtgccg
     3421 ggcggcccgc tcctggcggt cgtcggcgtc gtcgtcctgg tcgtcgtcct gctctcgccg
     3481 tcggcgtgca gttgcttcct cgcggcgctg ggcgagggcg gcgagcatgt cggcgtacgc
     3541 ctcggccacc tcccccgccg tgagcaccac cactgtgtcg gccgcgtcgg ccagcgccag
     3601 gacctcccgc acccgttcgc ccacggccgc cgaatcctcg ttgccgtcct tgccttcggc
     3661 ggcccgggtc gcctcgaggt cgagggcgcg gcgggtgacc gcgtgccatc cgtcctcggt
     3721 cacggcgacc ccggcccgca gctccccgcc gtcggcgtcg gccgccagga gcagatcgag
     3781 gtcgtcggcc tcggtgtcgc cgccgtcgag cccgagcatc tgccgcaggt agcgggtcca
     3841 ttcgatggcc cggcgtcccc gggttgcccg ctcgtactcg tgccagcgcg agaggttcca
     3901 ctccagcgag ccgaccccgg cggcgtcgtc ctcggtcatg ccgccggtca ggtccccgat
     3961 ccgtccgagg agttcgaacg gggcgacgtt cccgccggtc gccgtcttga ggtcggcgcg
     4021 ggcgagttcg agggcgggcg ccttcccgtc ctgggtcttg gcgatgtact cggcgaggtc
     4081 gttggcgtcg cgctcggtct ccagccgctt gaagtcgacg ccgtgccggt cgtcgggcgt
     4141 gaaggcgggg ttgaccttgc gcagggcggc ggtccacacg gaccgccagt gcccctgcca
     4201 ctcgtcgagc gcggcgccgg tcggctcgaa ggtggcgacg atctgcttcg cggaccgctc
     4261 cccctcggtc cggccgccga ccaggacgat cgcgtggatg tgcgggtgcc agccgttgat
     4321 ctgccccacg gtgacttcgg tcgcgcggat catgccgacg tacccgatcc ggtctcggat
     4381 gccctcgcgg tcggcggccc ggtgcccgtc cttggcccgg cgtccggccc acgtgccgcc
     4441 cgtgatcagt cgctggtagg cgcccggccg ccgggggctg tccggcgtct tccgggtgcc
     4501 ctggagggcg tccatgaggt ccgcgagccg gtccgtgtgc ccatggcggg ccgtgaaggt
     4561 gaccaggtag gcggtccccc cgcgcttgat ccactcgacc acggcggcgg tgatctcctc
     4621 ggcccgcttg tgccggatcg tggcggcgca gaccgggcag agccagatcc gcccgcaccg
     4681 catcaggccc aggaccacgg acgttccggc cgccgtctgg gcgacgatca cgccggaggc
     4741 agggtccatc agggcgcggc cgcagccctt gcacgcggcg tccccgctga tccgccacag
     4801 cgtccggcgg cggctgtacc gggcggcttt ccgcagtcgg gcagcgtcgc tccgcgacgt
     4861 gcttcctact tccgagaggc tgtcgcctct cgggctctcc ccatccaccc cgtccggaga
     4921 aaccgcaggt cggaggggtg cgggaaactc tgttgtttct ttcccaaggt gttcgctttt
     4981 gcctcgggcg gcatctcgcg tcacacgcgc gatcgcccgc ttcgctgcca tccggcagcg
     5041 gtctgagcag tagatacgcg gccgtttgcc cggtgtgtgg gcaattgcgg tcccgcagtg
     5101 gcagcggggc ccggcgggcc gatctggcaa tgcctcggca tcgctccgta ctctgggcac
     5161 gagcaacgtt cctgtctcgc ccggctaagg ggcgcgagtc tgggagcgga cgggtcggag
     5221 gtgcgaagtc cggcccgttg ctctttggtc tggtgggaat cctggcacca atcgggccag
     5281 aggttccctc cgccactccc gacgcccctt ggggctggtg tgacttggag ggccgaagag
     5341 agccccgccg gtgatccggc ggggctttga cgtgcggtca gtgcgtgtgt cggcgagcga
     5401 tggccacgag gccctggaag ccgagcggtc cggcgaagtc ggcccagtcg caaccgggct
     5461 cagcgcagtg ggcggaccag ccaccgccgt tggggtcctg gaccaggttc acggtcccct
     5521 cggtcaggcg tccgtcgaag tcggtcatgg tcggtctcct ggtgggtggg ggcggggcgc
     5581 cagcacgaag tgccggcgcc ccgcgggggt tggtcgggtc aggcgccgaa ccggcgggcg
     5641 gcggcggcga ccaggccgtc ggcggcggcc atggcgcggt cgcggtcggt ggtgagggcg
     5701 gtgcggtcgg cggcggccca gtcctgctct cacgcaacgt ctacgtggac gctcagggcg
     5761 acacgatcga ggtcggggag tccgtcgctc cggcgggccg ctggcgcgtc caccgggact
     5821 gatcaaggcg aatacttcat atggccctgc aggcggaagt caggtagaca cgacttccgc
     5881 tagtccttgc aaggtctgct gacgtgaggc ggggcggtcg tttttgaccg ccctgccttc
     5941 gtcatgtagg ctcgctcgct gtgcctggcg tgtcatcaga cgcccaggtc ccggtgccgt
     6001 gaggcccggg ccatcgagcc ggtggtacgt ggctgcggtc cccttgtgag ggctgcgcgc
     6061 cgtgtgctgt ccggcgcgca cagccttgaa tccacccgcg ggggccggcc ggtctccgtg
     6121 agctcgagta gacgacggag acgtaatgtc cgaggtggag ttctcccacg agtactggat
     6181 gcgccacgcc ctgaccctgg ccaagcgcgc gtgggacgag cgcgaggtgc cggtgggcgc
     6241 cgtgctggtc cacaacaacc gcgtgatcgg cgagggctgg aaccggccga tcggccggca
     6301 cgacccgacc gcccacgccg agatcatggc cctgcgccag ggcggcctgg tcatgcagaa
     6361 ctaccggctg atcgacgcga ccctgtacgt gaccctggag ccgtgcgtca tgtgcgcggg
     6421 cgccatgatc cactcccgca tcggccgggt ggtgttcggc gcccgggacg ccaagaccgg
     6481 cgccgcgggc tccctgatgg acgtgctgca ccacccgggc atgaaccacc gcgtcgagat
     6541 caccgagggc atcctggcgg acgagtgcgc cgcgctgctg tcggacttct tccggatgcg
     6601 ccggcaggag atcaaggccc agaagaaggc gcagtcgtcc accgactcgg gcggctcgtc
     6661 cggcggctcg tccggctccg agaccccggg cacctcggag tccgcgaccc cggagtcgtc
     6721 cggcggctcg tccggcggct cgtccgaggt cgaattttcc catgaatact ggatgcggca
     6781 cgcgctgacc ctggccaagc gggcccgcga cgagcgcgag gtgcccgtcg gcgcggtgct
     6841 ggtcctgaac aaccgggtca ttggtgaggg ctggaaccgc gccatcggcc tgcacgaccc
     6901 caccgcgcat gccgaaatca tggccctccg gcagggcggc ctggtgatgc agaactaccg
     6961 cctcatcgac gccaccctgt acgtgacctt cgagccctgc gtgatgtgtg cgggtgccat
     7021 gatccactcc cggatcggcc gcgtcgtgtt cggcgtgcgc aacgccaaga ccggcgcggc
     7081 cggctccctg atggacgtcc tgcactaccc cggcatgaac caccgcgtgg aaattaccga
     7141 gggcatcctg gccgacgagt gcgcggccct gctgtgctac ttcttccgca tgccgcggca
     7201 ggtgttcaac gcgcagaaga aggcgcagtc gtccacggat tcgggcggct cgtccggcgg
     7261 ctcgtccggg tcggaaaccc cgggtacctc ggagtcggcc accccggagt cgtccggcgg
     7321 ctcgtccggc ggctcggaca agaagtacag catcggcctg gccatcggca ccaacagcgt
     7381 gggctgggcg gtcatcaccg acgagtacaa ggtcccctcc aagaagttca aggtcctggg
     7441 caacaccgac cggcactcga tcaagaagaa cctgatcggc gccctgctct tcgacagcgg
     7501 cgagaccgcc gaggcgaccc gcctgaagcg gaccgcgcgc cggcgctaca cccggcgcaa
     7561 gaaccgcatc tgctacctcc aggaaatctt ctccaacgag atggccaagg tcgacgactc
     7621 gttcttccac cggctcgagg agagcttcct ggtggaggag gacaagaagc acgagcgcca
     7681 cccgatcttc ggcaacatcg tcgacgaggt ggcctaccac gagaagtacc ccaccatcta
     7741 ccacctccgc aagaagctgg tcgactcgac cgacaaggcg gacctgcggc tcatctacct
     7801 ggccctcgcg cacatgatca agttccgcgg ccacttcctc atcgagggcg acctgaaccc
     7861 ggacaactcc gacgtcgaca agctcttcat ccagctggtg cagacctaca accagctgtt
     7921 cgaggagaac cccatcaacg ccagcggcgt cgacgccaag gcgatcctct ccgcgcgcct
     7981 gagcaagtcc cggcgcctgg agaacctcat cgcccagctg ccgggcgaga agaagaacgg
     8041 cctcttcggc aacctgatcg cgctgtcgct cggcctgacc cccaacttca agagcaactt
     8101 cgacctggcc gaggacgcga agctccagct gtccaaggac acctacgacg acgacctgga
     8161 caacctgctc gcccagatcg gcgaccagta cgcggacctc ttcctggccg cgaagaacct
     8221 ctcggacgcc atcctgctca gcgacatcct gcgggtcaac accgagatca ccaaggcccc
     8281 gctgtcggcg agcatgatca agcggtacga cgagcaccac caggacctga ccctgctcaa
     8341 ggccctcgtg cgccagcagc tgcccgagaa gtacaaggaa atcttcttcg accagtccaa
     8401 gaacggctac gccggctaca tcgacggcgg cgcgtcgcag gaggagttct acaagttcat
     8461 caagccgatc ctggagaaga tggacggcac cgaggagctg ctcgtcaagc tgaaccgcga
     8521 ggacctgctc cgcaagcagc ggaccttcga caacggctcc atcccgcacc agatccacct
     8581 gggcgagctg cacgccatcc tccggcgcca ggaggacttc taccccttcc tgaaggacaa
     8641 ccgcgagaag atcgagaaga tcctgacctt ccgcatcccg tactacgtcg gccccctggc
     8701 ccgcggcaac tcccggttcg cgtggatgac ccggaagtcg gaggagacca tcaccccgtg
     8761 gaacttcgag gaggtcgtgg acaagggcgc ctccgcccag tcgttcatcg agcgcatgac
     8821 caacttcgac aagaacctcc cgaacgagaa ggtcctgccc aagcactccc tgctctacga
     8881 gtacttcacc gtgtacaacg agctgaccaa ggtcaagtac gtgaccgagg gcatgcggaa
     8941 gccggccttc ctgtcgggcg agcagaagaa ggcgatcgtc gacctgctct tcaagaccaa
     9001 ccgcaaggtc accgtgaagc agctgaagga ggactacttc aagaagatcg agtgcttcga
     9061 ctccgtcgag atcagcggcg tggaggaccg cttcaacgcc tccctgggca cctaccacga
     9121 cctgctcaag atcatcaagg acaaggactt cctcgacaac gaggagaacg aggacatcct
     9181 ggaggacatc gtcctcaccc tgaccctctt cgaggaccgc gagatgatcg aggagcggct
     9241 caagacctac gcccacctgt tcgacgacaa ggtgatgaag cagctgaagc ggcgccggta
     9301 caccggctgg ggccgcctct cccggaagct gatcaacggc atccgggaca agcagagcgg
     9361 caagaccatc ctggacttcc tcaagtccga cggcttcgcc aaccgcaact tcatgcagct
     9421 catccacgac gacagcctga ccttcaagga ggacatccag aaggcccagg tctcgggcca
     9481 gggcgacagc ctccacgagc acatcgccaa cctggcgggc tccccggcga tcaagaaggg
     9541 catcctccag accgtcaagg tcgtggacga gctggtcaag gtgatgggcc gccacaagcc
     9601 cgagaacatc gtgatcgaga tggcccggga gaaccagacc acccagaagg gccagaagaa
     9661 ctcgcgcgag cggatgaagc ggatcgagga gggcatcaag gagctgggca gccagatcct
     9721 gaaggagcac ccggtcgaga acacccagct ccagaacgag aagctgtacc tctactacct
     9781 ccagaacggc cgcgacatgt acgtggacca ggagctggac atcaaccggc tgtccgacta
     9841 cgacgtcgac cacatcgtgc cgcagtcctt cctgaaggac gactcgatcg acaacaaggt
     9901 cctgacccgc tcggacaaga accggggcaa gtccgacaac gtgccctcgg aggaggtcgt
     9961 gaagaagatg aagaactact ggcgccagct gctcaacgcc aagctcatca cccagcgcaa
    10021 gttcgacaac ctgaccaagg ccgagcgggg cggcctgagc gagctggaca aggcgggctt
    10081 catcaagcgc cagctggtcg agacccggca gatcaccaag cacgtggccc agatcctgga
    10141 ctcccggatg aacaccaagt acgacgagaa cgacaagctg atccgcgagg tcaaggtgat
    10201 caccctcaag agcaagctgg tctccgactt ccgcaaggac ttccagttct acaaggtccg
    10261 ggagatcaac aactaccacc acgcccacga cgcgtacctg aacgccgtcg tgggcaccgc
    10321 gctgatcaag aagtacccga agctggagtc cgagttcgtc tacggcgact acaaggtcta
    10381 cgacgtgcgc aagatgatcg ccaagagcga gcaggagatc ggcaaggcca ccgcgaagta
    10441 cttcttctac tccaacatca tgaacttctt caagaccgag atcaccctgg ccaacggcga
    10501 gatccgcaag cggcccctga tcgagaccaa cggcgagacc ggcgagatcg tctgggacaa
    10561 gggccgcgac ttcgccaccg tccggaaggt gctgtcgatg ccgcaggtca acatcgtgaa
    10621 gaagaccgag gtgcagaccg gcggcttcag caaggagtcc atcctcccca agcgcaacag
    10681 cgacaagctg atcgcccgga agaaggactg ggacccgaag aagtacggcg gcttcgacag
    10741 ccccaccgtc gcctactccg tgctggtcgt ggcgaaggtc gagaagggca agagcaagaa
    10801 gctgaagtcc gtgaaggagc tgctcggcat caccatcatg gagcgctcct cgttcgagaa
    10861 gaacccgatc gacttcctgg aggccaaggg ctacaaggag gtcaagaagg acctcatcat
    10921 caagctgccc aagtacagcc tgttcgagct ggagaacggc cgcaagcgga tgctcgcctc
    10981 cgcgggcgag ctgcaaaagg gcaacgagct ggccctcccg tcgaagtacg tcaacttcct
    11041 gtacctcgcg tcccactacg agaagctgaa gggctcgccc gaggacaacg agcagaagca
    11101 gctcttcgtg gagcagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
    11161 cagcaagcgc gtcatcctgg ccgacgcgaa cctcgacaag gtgctgtccg cctacaacaa
    11221 gcaccgcgac aagccgatcc gggagcaggc ggagaacatc atccacctgt tcaccctcac
    11281 caacctgggt gccccggccg ccttcaagta cttcgacacc accatcgacc gcaagcggta
    11341 cacctccacc aaggaggtcc tcgacgcgac cctgatccac cagagcatca ccggcctgta
    11401 cgagacccgc atcgacctga gccagctggg cggcgactga gaattcagat ctacgcgttc
    11461 cccgcaaaag cggcctttga ctccctgcaa gcctcagcga ccgaatatat cggttatgcg
    11521 tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc
    11581 acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag ccttttttta
    11641 agcttgcatg cctgcaggtc gacggatctt ttccgctgca taaccctgct tcggggtcat
    11701 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
    11761 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
    11821 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
    11881 caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa
    11941 gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca aggtgtactg
    12001 ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca tgagcctgtc
    12061 ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg actatgagca
    12121 cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc tgctgaaact
    12181 ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc tcgccctgct
    12241 ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg tggtccgccc
    12301 gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc gtgattgcca
    12361 agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg aagtacatca
    12421 ccgacgagca aggcaagacc gatccccggg gacctgcagg tcgactctag agttttagag
    12481 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
    12541 tcggtgctaa aaaaaaaccc cgcccctgac agggcggggt tttttttgta gcctcaggag
    12601 ttgtagctgt tcacattcga acggtctctg ctttgacaac atgctgtgcg gtgttgtaaa
    12661 gtcgtggccg ctagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    12721 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
    12781 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
    12841 cccggacgag cgtctgctcc gccattcgcc gtccgccgtg ccaatcggat cagccgtcca
    12901 aatgcgggat tttcgttagt cggaggccaa acggcattga gcgtcagcat atcatcagcg
    12961 agctgaagaa agacaatccc cgatccgctc cacgtgttgc cccagcaatc agcgcgacct
    13021 tgcccctcca acgtcatctc gttctccgct catgagctca gccaatcgac tggcgagcgg
    13081 catcgcattc ttcgcatccc gcctctggcg gatgcaggaa gatcaacgga tctcggccca
    13141 gttgacccag ggctgtcgcc acaatgtcgc gggagcggat caaccgagca aaggcatgac
    13201 cgactggacc ttccttctga aggctcttct ccttgagcca cctgtccgcc aaggcaaagc
    13261 gctcacagca gtggtcattc tcgagataat cgacgcgtac caacttgcca tcctgaagaa
    13321 tggtgcagtg tctcggcacc ccatagggaa cctttgccat caactcggca agatgcagcg
    13381 tcgtgttggc atcgtgtccc acgccgagga gaagtacctg cccatcgagt tcatggacac
    13441 gggcgaccgg gcttgcaggc gagtgaggtg gcaggggcaa tggatcagag atgatctgct
    13501 ctgcctgtgg ccccgctgcc gcaaaggcaa atggatgggc gctgcgcttt acatttggca
    13561 ggcgccagaa tgtgtcagag acaactccaa ggtccggtgt aacgggcgac gtggcaggat
    13621 cgaacggctc gtcgtccaga cctgaccacg agggcatgac gagcgtccct cccggaccca
    13681 gcgcagcacg cagggcctcg atcagtccaa gtggcccatc ttcgaggggc cggacgctac
    13741 ggaaggagct gtggaccagc agcacaccgc cgggggtaac cccaaggttg agaagctgac
    13801 cgatg
//
