LOCUS Exported 6287 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression of human GATA3. ACCESSION . VERSION . KEYWORDS RSV/hGATA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6287) AUTHORS Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD TITLE Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. JOURNAL Mol Cell Biol. 1991 May . 11(5):2778-84. PUBMED 2017177 REFERENCE 2 (bases 1 to 6287) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6287 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(7..268) /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" primer_bind 545..564 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(645..662) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(816..1404) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(896..915) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1575..2435) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2198..2217 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2436..2540) /gene="bla" /label=AmpR promoter primer_bind 2608..2626 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" polyA_signal 3393..3527 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3417..3436) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3471..3490 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(3952..3972) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 4102..4167 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" ORIGIN 1 cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat cgagctaggc 61 acttaaatac aatatctctg caatgcggaa ttcagtggtt cgtccaatcc atgtcagacc 121 cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc 181 acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga 241 ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg aaactactat 301 atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct aaacacaccc 361 tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta 421 accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt 481 ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac 541 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 601 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 1021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 1081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 1141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 1201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 1261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 1321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 1381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 1441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1501 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1561 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 1621 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 1681 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 1741 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 1801 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 1861 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 1921 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 1981 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 2041 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 2101 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 2161 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 2221 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 2281 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 2341 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 2401 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 2461 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 2521 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 2581 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 2641 gaattccttt gcctaattta aatgaggact taacctgtgg aaatattttg atgtgggaag 2701 ctgttactgt taaaactgag gttattgggg taactgctat gttaaacttg cattcaggga 2761 cacaaaaaac tcatgaaaat ggtgctggaa aacccattca agggtcaaat tttcattttt 2821 ttgctgttgg tggggaacct ttggagctgc agggtgtgtt agcaaactac aggaccaaat 2881 atcctgctca aactgtaacc ccaaaaaatg ctacagttga cagtcagcag atgaacactg 2941 accacaaggc tgttttggat aaggataatg cttatccagt ggagtgctgg gttcctgatc 3001 caagtaaaaa tgaaaacact agatattttg gaacctacac aggtggggaa aatgtgcctc 3061 ctgttttgca cattactaac acagcaacca cagtgcttct tgatgagcag ggtgttgggc 3121 ccttgtgcaa agctgacagc ttgtatgttt ctgctgttga catttgtggg ctgtttacca 3181 acacttctgg aacacagcag tggaagggac ttcccagata ttttaaaatt acccttagaa 3241 agcggtctgt gaaaaacccc tacccaattt cctttttgtt aagtgaccta attaacagga 3301 ggacacagag ggtggatggg cagcctatga ttggaatgtc ctctcaagta gaggaggtta 3361 gggtttatga ggacacagag gagcttcctg gggatccaga catgataaga tacattgatg 3421 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 3481 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt 3541 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa 3601 acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat acatcaaata 3661 ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt 3721 attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata 3781 actgttatgc ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag 3841 tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac 3901 agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct 3961 cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg 4021 catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc 4081 atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca 4141 ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc 4201 caattatgtc acaccacaga agtaaggttc cttcacaaag atcaattccc ttttttttat 4261 tttcttttaa tgcatcaaac aactgtggcc agtgaaagga aacaaaactg gcagtttgtc 4321 catttgaata tcagacctag tttcttctta atttccacac catttctccc atattcctta 4381 aacttcttgg catccttcat gccttacagc tacccagatg caataaagtc attgtacagt 4441 atttcttaca atataagtta tatgcaatgt tcagcatttt tttttttcac agcactagag 4501 accctgttaa ataggggata tgagtcagaa tggcttattc acagatgggg tccagattca 4561 gtggttggaa cacagacacc acagtgagct cctttgcaaa gtggcaaaca taattttgct 4621 ttctgccttc aaaaacatat atccatcgcg tttaggcttc atgatactgc tcctgcaaaa 4681 atgcaagtcg aaagggactg cagggactct cgctgggggc cctgtgagca tcgagcaggg 4741 ctctaaccca tggcggtgac catgctggag gggtggtgtg gtccaaagga caggctggat 4801 ggcgggtgca tcggcgtggg cgtggtcagc atgtggctgg agtggctgaa gggcgagatg 4861 tggctcaggg aggacatgtg tctggagagg gcggccgggt taaacgagct gttcttgggg 4921 aagtcctcca gtgagtcatg cacttttttg cactttttgg atttgctaga catttttcgg 4981 tttctggtct ggatgccttc cttcttcata gtcaggggtc tgttaatatt gtgaagcttg 5041 tagtagagcc cacaggcatt gcagacaggg tccccattgg cattcctcct ccagagtgtg 5101 gttgtggtgg tctgacagtt cgcacaggac gtccctgctc tcctggctgc agacagcctt 5161 cgcttgggct taatgagggg ccggttctgt ccgttcattt tgtgatagag cccgcaggcg 5221 ttgcacaggt agtgtcccgt gccatctcgc cgccacagtg gggtcgaggt tgccccacag 5281 ttcacacact ccctgccttc tgtgctggac cgggccttgg gcctggactt gcatccgaag 5341 ccggtggggg agccgcccag caggctgctg ggggggaaga gtccggagct gtactcgggc 5401 acgtagggcg ggtaggtggt gatggggtgg tgggtcgacg aggaggctcc acccagggcg 5461 gtcatgctgc cacgggagtg ggacgactcc agcttcatgc tgtcgggcag gggcacctgg 5521 tacttgaggc actctttctc gtcctgccgg gccgagccgg ccgagcctgg ggtggacagc 5581 gatgggtccg gggagacgtc cttcggcggg gtgggcggga aggtgaagag gtgcgggctg 5641 gcgtggcccc ccgacaagga ggaggacgag gccggggggt agacggagag gggccccggg 5701 gagccgtggt ggatggacgt cttggagaag gggctgagat tccaggggga ggcggtgtgg 5761 tggctgccca gggctttgcc gccgtccagc cagggtaggg atccatgaag cagaggcggg 5821 cggcacacct ggctcccgtg gtgggtcgga gggtacctct gcaccgtggc cctgaccgag 5881 tttccgtagt agggcgggac gtggttgcct tgaccgtcga tgttaaaaag cacatccacc 5941 tcctccggca gcgggtactg cgccgcgtcc atgtaggagt ggctgaggcc cgggtggtgc 6001 gtgtccgggt gctgcccgtt gagcacggcg gggtggtggt ggctcaccca gcgcggctgg 6061 tccgccgtca cctccatggc ctcggctgtg ctcgcgccct ctcgccgggc ccggaacccg 6121 cgcgcgggga gggagggcgg tccgcctggg aggtcggggg gctcctgccg tcggagggtc 6181 gggggtcgtt gaatgatttg ctttcggtgg ggggatggga aggctgggaa gcaaaggtga 6241 gcaaaggaga aggtttttta aaaattatct tggagggggg gaattag //