LOCUS Exported 4478 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.. ACCESSION . VERSION . KEYWORDS pCAMKIIa-TetOn3G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4478) AUTHORS Heumuller M, Glock C, Rangaraju V, Biever A, Schuman EM TITLE A genetically encodable cell-type-specific protein synthesis inhibitor. JOURNAL Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0468-x. doi: 10.1038/s41592-019-0468-x. PUBMED 31308551 REFERENCE 2 (bases 1 to 4478) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4478 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" primer_bind 595..614 /label=T7 /note="T7 promoter, forward primer" promoter 595..613 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 667..1413 /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /label=Tet-On(R) 3G /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" primer_bind complement(1426..1443) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1432..1656 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 1741..1881 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 1741..1870 /label=AAV2 ITR rep_origin 1956..2411 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2043..2062) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2253..2274 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2428..2447) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2547..2569 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2607..2625) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2693..2797 /gene="bla" /label=AmpR promoter CDS 2798..3658 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3016..3035) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3829..4417 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4318..4337 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggcaattc agtcgataac tataacggtc ctaaggtagc gatttaaata 181 cgcgctctct taaggtagcc ccgggacgcg tcaattgcat gaagaatctg cttaggatgt 241 ggactaagtt tgttcacatc cccttctcca accccctcag tacatcaccc tgggggaaca 301 gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa ggccagggca 361 aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt taccggggca 421 acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga cgaaggctcg 481 cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca gtcaagccgg 541 ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg ggagtaatac 601 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc 661 ggatccatgt ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat 721 ggagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag 781 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag 841 atgctggaca ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt 901 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa 961 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg 1021 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc 1081 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga 1141 gagacaccta ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac 1201 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag 1261 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac 1321 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt 1381 gacgattttg accttgacat gctccccggg taacccgctg atcagcctcg actgtgcctt 1441 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 1501 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 1561 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 1621 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagat 1681 cctctcttaa ggtagcatcg agatttaaat tagggataac agggtaatgg cgcgggccgc 1741 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 1801 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 1861 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg 1921 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag 1981 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc 2041 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 2101 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 2161 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 2221 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 2281 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta 2341 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 2401 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2461 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2521 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2581 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 2641 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 2701 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 2761 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 2821 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 2881 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 2941 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 3001 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 3061 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 3121 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 3181 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 3241 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 3301 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 3361 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 3421 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 3481 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 3541 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 3601 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 3661 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 3721 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 3781 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 3841 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 3901 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 3961 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 4021 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 4081 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 4141 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 4201 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 4261 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 4321 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 4381 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 4441 acggttcctg gccttttgct ggccttttgc tcacatgt //