LOCUS Exported 11135 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS myc-NLRC5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11135) AUTHORS Neerincx A, Rodriguez GM, Steimle V, Kufer TA TITLE NLRC5 Controls Basal MHC Class I Gene Expression in an MHC Enhanceosome-Dependent Manner. JOURNAL J Immunol. 2012 May 15;188(10):4940-50. Epub 2012 Apr 6. PUBMED 22490867 REFERENCE 2 (bases 1 to 11135) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11135 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 689..718 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 722..751 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 755..784 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 788..811 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" primer_bind complement(6492..6509) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6498..6722 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6768..7196 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6855..6874) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7065..7086 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7205..7225) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7210..7539 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7390..7525 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7452..7471 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7606..8400 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7660..7679) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8270..8289 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 8574..8695 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8611..8630) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8665..8684 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8744..8760) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8744..8760) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8757..8779) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8768..8784 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8792..8822) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8837..8858 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8975..8992) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9146..9734) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9226..9245) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9905..10765) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10528..10547 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10766..10870) /gene="bla" /label=AmpR promoter primer_bind complement(10945..10964) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagcgttt aaacttaagc ttaccatgga gcagaagctg atctccgagg aggacctgaa 721 cgagcagaag ctgatctccg aggaggacct gaacgagcag aagctgatct ccgaggagga 781 cctgaacctg gaggtgctgt tccagggacc cggggtacct aaggatccag tgtggtggaa 841 ttctgcagat atcatggacc ccgttggcct ccagctcggc aacaagaacc tgtggagctg 901 tcttgtgagg ctgctcacca aagacccaga atggctgaac gccaagatga agttcttcct 961 ccccaacacg gacctggatt ccaggaacga gaccttggac cctgaacaga gagtcatcct 1021 gcaactcaac aagctgcatg tccagggttc ggacacctgg cagtctttca ttcattgtgt 1081 gtgcatgcag ctggaggtgc ctctggacct ggaggtgctg ctgctgagta cttttggcta 1141 tgatgatggg ttcaccagcc agctgggagc tgaggggaaa agccaacctg aatctcagct 1201 ccaccatggc ctgaagcgcc cacatcagag ctgtgggtcc tcaccccgcc ggaagcagtg 1261 caagaagcag cagctagagt tggccaagaa gtacctgcag ctcctgcgga cctctgccca 1321 gcagcgctac aggagccaaa tccctgggtc agggcagccc cacgccttcc accaggtcta 1381 tgtccctcca atcctgcgcc gggccacagc atccttagac actccggagg gggccattat 1441 gggggacgtc aaggtggaag atggtgctga cgtgagcatc tcggacctct tcaacaccag 1501 ggttaacaag ggcccgaggg tgaccgtgct tttggggaag gctggcatgg gcaagaccac 1561 gctggcccac cggctctgcc agaagtgggc agagggccat ctgaactgtt tccaggccct 1621 gttccttttt gaattccgcc agctcaactt gatcacgagg ttcctgacac cgtccgagct 1681 cctttttgat ctgtacctga gccctgaatc ggaccacgac actgtcttcc agtacctgga 1741 gaagaacgct gaccaagtcc tgctgatctt tgatgggcta gatgaggccc tccagcctat 1801 gggtcctgat ggcccaggcc cagtcctcac ccttttctcc catctctgca atgggaccct 1861 cctgcctggc tgccgggtga tggctacctc ccgtccaggg aagctgcctg cctgcctgcc 1921 tgcagaggca gccatggtcc acatgttggg ctttgatggg ccacgggtgg aagaatatgt 1981 gaatcacttc ttcagcgccc agccatcgcg ggagggggcc ctggtggagt tacagacaaa 2041 tggacgtctc cgaagcctgt gtgcggtgcc cgcactgtgc caagtcgcct gtctttgcct 2101 ccaccatctg cttcctgacc acgccccagg ccagtctgtg gccctcctgc ccaacatgac 2161 tcagctctat atgcagatgg tgctcgccct cagcccccct gggcacttgc ccacctcgtc 2221 cctactggac ctgggggagg tggccctgag gggcctggag acagggaagg ttatcttcta 2281 tgcaaaagat attgctccac ccttgatagc ttttggggcc actcacagcc tgctgacttc 2341 cttctgcgtc tgcacaggcc ctgggcacca gcagacaggc tatgctttca cccacctcag 2401 cctgcaggag tttcttgctg ccctgcacct gatggccagc cccaaggtga acaaagacac 2461 acttacccag tatgttaccc tccattcccg ctgggtacag cggaccaaag ctagactggg 2521 cctctcagac cacctcccca ccttcctggc gggcctggca tcctgcacct gccgcccctt 2581 ccttagccac ctggcgcagg gcaatgagga ctgtgtgggt gccaagcagg ctgctgtagt 2641 gcaggtgttg aagaagttgg ccacccgcaa gctcacaggg ccaaaggttg tagagctgtg 2701 tcactgtgtg gatgagacac aggagcctga gctggccagt ctcaccgcac aaagcctccc 2761 ctatcaactg cccttccaca atttcccact gacctgcacc gacctggcca ccctgaccaa 2821 catcctagag cacagggagg cccccatcca cctggatttt gatggctgtc ccctggagcc 2881 ccactgccct gaggctctgg taggctgtgg gcagatagag aatctcagct ttaagagcag 2941 gaagtgtggg gatgcctttg cagaagccct ctccaggagc ttgccgacaa tggggaggct 3001 gcagatgctg gggttagcag gaagtaaaat cactgcccga ggcatcagcc acctggtgaa 3061 agctttgcct ctctgtccac agctgaaaga agtcagtttt cgggacaacc agctcagtga 3121 ccaggtggtg ctgaacattg tggaggttct ccctcaccta ccacggctcc ggaagcttga 3181 cctgagcagc aacagcatct gcgtgtcaac cctactctgc ttggcaaggg tggcagtcac 3241 gtgtcctacc gtcaggatgc ttcaggccag ggaggcggac ctcatcttcc ttctttcccc 3301 gcccacagag acaactgcag agctacaaag agctccagac ctgcaggaaa gtgacggcca 3361 gaggaaaggg gctcagagca gaagcttgac gctcaggctg cagaagtgtc agctccaggt 3421 ccacgatgcg gaggccctca tagccctgct ccaggaaggc cctcacctgg aggaagtgga 3481 cctctcaggg aaccagctgg aagatgaagg ctgtcggctg atggcagagg ctgcatccca 3541 gctgcacatc gccaggaagc tggacctcag cgacaacggg ctttctgtgg ccggggtgca 3601 ttgtgtgctg agggccgtga gtgcgtgctg gaccctggca gagctgcaca tcagcctgca 3661 gcacaaaact gtgatcttca tgtttgccca ggagccagag gagcagaagg ggccccagga 3721 gagggctgca tttcttgaca gcctcatgct ccagatgccc tctgagctgc ctctgagctc 3781 ccgaaggatg aggctgacac attgtggcct ccaagaaaag cacctagagc agctctgcaa 3841 ggctctggga ggaagctgcc acctcggtca cctccacctc gacttctcag gcaatgctct 3901 gggggatgaa ggtgcagccc ggctggctca gctgctccca gggctgggag ctctgcagtc 3961 cttgaacctc agtgagaacg gtttgtccct ggatgccgtg ttgggtttgg ttcggtgctt 4021 ctccactctg cagtggctct tccgcttgga catcagcttt gaaagccaac acatcctcct 4081 gagaggggac aagacaagca gggatatgtg ggccactgga tctttgccag acttcccagc 4141 tgcagccaag ttcttagggt tccgtcagcg ctgcatcccc aggagcctct gcctcagtga 4201 gtgtcctctg gagcccccaa gcctcacccg cctctgtgcc actctgaagg actgcccggg 4261 acccctggaa ctgcaattgt cctgtgagtt cctgagtgac cagagcctgg agactctact 4321 ggactgctta cctcaactcc ctcagctgag cctgctgcag ctgagccaga cgggactgtc 4381 cccgaaaagc cccttcctgc tggccaacac cttaagcctg tgtccacggg ttaaaaaggt 4441 ggatctcagg tccctgcacc atgcaacttt gcacttcaga tccaacgagg aggaggaagg 4501 cgtgtgctgt ggcaggttca caggctgcag cctcagccag gagcacgtag agtcactctg 4561 ctggttgctg agcaagtgta aagacctcag ccaggtggat ctctcagcaa acctgctggg 4621 cgacagcgga ctcagatgcc ttctggaatg tctgccgcag gtgcccatct ccggtttgct 4681 tgatctgagt cacaacagca tttctcagga aagtgccctg tacctgctgg agacactgcc 4741 ctcctgccca cgtgtccggg aggcctcagt gaacctgggc tctgagcaga gcttccggat 4801 tcacttctcc agagaggacc aggctgggaa gacactcagg ctaagtgagt gcagcttccg 4861 gccagagcac gtgtccaggc tggccaccgg cttgagcaag tccctgcagc tgacggagct 4921 cacgctgacc cagtgctgcc tgggccagaa gcagctggcc atcctcctga gcttggtggg 4981 gcgacccgca gggctgttca gcctcagggt gcaggagccg tgggcggaca gagccagggt 5041 tctctccctg ttagaagtct gcgcccaggc ctcaggcagt gtcactgaaa tcagcatctc 5101 cgagacccag cagcagctct gtgtccagct ggaatttcct cgccaggaag agaatccaga 5161 agctgtggca ctcaggttgg ctcactgtga ccttggagcc caccacagcc ttcttgtcgg 5221 gcagctgatg gagacatgtg ccaggctgca gcagctcagc ttgtctcagg ttaacctctg 5281 tgaggacgat gatgccagtt ccctgctgct gcagagcctc ctgctgtccc tctctgagct 5341 gaagacattt cggctgacct ccagctgtgt gagcaccgag ggcctcgccc acctggcatc 5401 tggtctgggc cactgccacc acttggagga gctggacttg tctaacaatc aatttgatga 5461 ggagggcacc aaggcgctga tgagggccct tgaggggaaa tggatgctaa agaggctgga 5521 cctcagtcac cttctgctga acagctccac cttggccttg cttactcaca gactaagcca 5581 gatgacctgc ctgcagagcc tcagactgaa caggaacagt atcggtgatg tcggttgctg 5641 ccacctttct gaggctctca gggctgccac cagcctagag gagctggact tgagccacaa 5701 ccagattgga gacgctggtg tccagcactt agctaccatc ctgcctgggc tgccagagct 5761 caggaagata gacctctcag ggaatagcat cagctcagcc gggggagtgc agttggcaga 5821 gtctctcgtt ctttgcaggc gcctggagga gttgatgctt ggctgcaatg ccctggggga 5881 tcccacagcc ctggggctgg ctcaggagct gccccagcac ctgagggtcc tacacctacc 5941 attcagccat ctgggcccag gtggggccct gagcctggcc caggccctgg atggatcccc 6001 ccatttggaa gagatcagct tggcggaaaa caacctggct ggaggggtcc tgcgtttctg 6061 tatggagctc ccgctgctca gacagataga cctggtttcc tgtaagattg acaaccagac 6121 tgccaagctc ctcacctcca gcttcacgag ctgccctgcc ctggaagtaa tcttgctgtc 6181 ctggaatctc ctcggggatg aggcagctgc cgagctggcc caggtgctgc cgcagatggg 6241 ccggctgaag agagtggacc tggagaagaa tcagatcaca gctttggggg cctggctcct 6301 ggctgaagga ctggcccagg ggtctagcat ccaagtcatc cgcctctgga ataaccccat 6361 tccctgcgac atggcccagc acctgaagag ccaggagccc aggctggact ttgccttctt 6421 tgacaaccag ccccaggccc cttggggtac ttgactcgag tctagagggc ccgtttaaac 6481 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 6541 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 6601 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 6661 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 6721 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 6781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 6841 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 6901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 6961 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 7021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 7081 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 7141 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 7201 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 7261 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 7321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 7381 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 7441 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 7501 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 7561 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 7621 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 7681 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 7741 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 7801 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 7861 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 7921 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 7981 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 8041 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 8101 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 8161 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 8221 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 8281 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 8341 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 8401 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 8461 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 8521 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 8581 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 8641 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 8701 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 8761 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 8821 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 8881 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 8941 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 9001 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 9061 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 9121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 9181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 9241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 9301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 9361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 9421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 9481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 9541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 9601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 9661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 9721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 9781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 9841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 9901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 9961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 10021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 10081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 10141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 10201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 10261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 10321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 10381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 10441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 10501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 10561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 10621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 10681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 10741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 10801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 10861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc 10921 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta 10981 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 11041 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc 11101 gctgcttcgc gatgtacggg ccagatatac gcgtt //