LOCUS Exported 5619 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS GFP-TREX1(D18N) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5619) AUTHORS Yan N, Regalado-Magdos AD, Stiggelbout B, Lee-Kirsch MA, Lieberman J TITLE The cytosolic exonuclease TREX1 inhibits the innate immune response to human immunodeficiency virus type 1. JOURNAL Nat Immunol. 2010 Nov . 11(11):1005-13. PUBMED 20871604 REFERENCE 2 (bases 1 to 5619) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5619 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 613..1329 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 613..1329 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(658..679) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(919..938) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1266..1287 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 2407..2528 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2444..2463) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2498..2517 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2535..2990) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2672..2693) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2884..2903 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3017..3121 /gene="bla" /label=AmpR promoter promoter 3123..3480 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3146..3166) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3331..3466 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3393..3412 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3515..4309 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3569..3588) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4179..4198 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4497..4516) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4541..4588 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4917..5505 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5406..5425 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctatgggc tcgcaggccc tgcccccggg gcccatgcag 1381 accctcatct ttttcaacat ggaggccact ggcttgccct tctcccagcc caaggtcacg 1441 gagctgtgcc tgctggctgt ccacagatgt gccctggaga gcccccccac ctctcagggg 1501 ccacctccca cagttcctcc accaccgcgt gtggtagaca agctctccct gtgtgtggct 1561 ccggggaagg cctgcagccc tgcagccagc gagatcacag gtctgagcac agctgtgctg 1621 gcagcgcatg ggcgtcaatg ttttgatgac aacctggcca acctgctcct agccttcctg 1681 cggcgccagc cacagccctg gtgcctggtg gcacacaatg gtgaccgcta cgacttcccc 1741 ctgctccaag cagagctggc tatgctgggc ctcaccagtg ctctggatgg tgccttctgt 1801 gtggatagca tcactgcgct gaaggccctg gagcgagcaa gcagcccctc agaacacggc 1861 ccaaggaaga gctacagcct aggcagcatc tacactcgcc tgtatgggca gtcccctcca 1921 gactcgcaca cggctgaggg tgatgtcctg gccctgctca gcatctgtca gtggagacca 1981 caggccctgc tgcggtgggt ggatgctcac gccaggcctt tcggcaccat caggcccatg 2041 tatggggtca cagcctctgc taggaccaag ccaagaccat ctgctgtcac aaccactgca 2101 cacctggcca caaccaggaa cactagtccc agccttggag agagcagggg taccaaggat 2161 cttcctccag tgaaggaccc tggagcccta tccagggagg ggctgctggc cccactgggt 2221 ctgctggcca tcctgacctt ggcagtagcc acactgtatg gactatccct ggccacacct 2281 ggggagtagt ctagataact gatcataatc agccatacca catttgtaga ggttttactt 2341 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 2401 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 2461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 2521 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 2581 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 2641 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 2701 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 2761 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 2821 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 2881 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 2941 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 3001 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 3061 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 3121 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 3181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 3241 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3301 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 3361 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 3421 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 3481 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 3541 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 3601 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 3661 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 3721 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 3781 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 3841 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 3901 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 3961 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 4021 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 4081 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 4141 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 4201 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 4261 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 4321 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 4381 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 4441 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 4501 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 4561 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 4621 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 4681 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 4741 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 4801 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 4861 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 4921 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 4981 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 5041 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 5101 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 5161 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 5221 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 5281 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 5341 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 5401 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 5461 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 5521 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 5581 tcccctgatt ctgtggataa ccgtattacc gccatgcat //