LOCUS       Exported                3984 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Red fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    pME tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      54..81
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      173..259
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     308..330
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     322..339
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     323..339
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    355..454
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             460..1890
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(1895..1994)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2011..2030)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2012..2030)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2035..2051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2035..2051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2153..2172)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2164..2973
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2231..2250)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3120..3708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3609..3628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3862..3879
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
       61 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
      121 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
      181 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
      241 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
      301 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
      361 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
      421 tttataatgc caactttgta caaaaaagca ggctggacca tggtgagcaa gggcgaggag
      481 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
      541 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      601 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
      661 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
      721 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
      781 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
      841 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      901 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
      961 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     1021 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     1081 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg
     1141 catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac
     1201 atggccgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     1261 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     1321 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     1381 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     1441 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     1501 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     1561 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1621 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     1681 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     1741 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     1801 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     1861 ctgtacggca tggacgagct ctacaagtag atctacccag ctttcttgta caaagttggc
     1921 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1981 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2041 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2101 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2161 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2221 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2281 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2341 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2401 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2461 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2521 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2581 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2641 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2701 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2761 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2821 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2881 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2941 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3001 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3061 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3781 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3841 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3901 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3961 cccgactgga aagcgggcag tgag
//
