LOCUS       Exported                4044 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Red fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    pME tdTomato-CAAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      100..127
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      219..305
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     354..376
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     368..385
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     369..385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    401..500
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             506..1933
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(2001..2100)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2117..2136)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2118..2136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2141..2157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2141..2157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2259..2278)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2270..3079
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2337..2356)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3226..3814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3715..3734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3968..3985
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
       61 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
      121 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
      181 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
      241 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
      301 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
      361 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
      421 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
      481 tttgtacaaa aaagcaggct ggaccatggt gagcaagggc gaggaggtca tcaaagagtt
      541 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
      601 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      661 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
      721 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
      781 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
      841 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
      901 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
      961 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     1021 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     1081 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     1141 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     1201 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     1261 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     1321 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1381 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     1441 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1501 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1561 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     1621 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1681 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     1741 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     1801 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     1861 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     1921 cgagctctac aagaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa
     1981 gtgtgtgctc tcctagatct acccagcttt cttgtacaaa gttggcatta taagaaagca
     2041 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2101 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2161 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2221 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2281 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2341 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2401 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2461 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2521 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2581 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     2641 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     2701 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2761 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     2821 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     2881 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     2941 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3001 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3061 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3121 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3181 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3241 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3301 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3361 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3421 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3481 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3541 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3601 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3661 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3721 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3781 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3841 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3901 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3961 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     4021 ccccgcgcgt tggccgattc atta
//
