LOCUS       Exported               11184 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses APOB-Linker-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Walsh MT, Celestin OM, Thierer JH, Rajan S, Farber SA, Hussain MM
  TITLE     Model systems to study assembly, trafficking and secretion of 
            apoB-lipoproteins using fluorescent fusion proteins.
  JOURNAL   J Lipid Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 
            10.1194/jlr.RA119000259.
  PUBMED    31888978
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    29..49
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        110..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        414..617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     568..588
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    638..662
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7374..8093
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7374..8090
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7419..7440)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7680..7699)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8027..8048
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(8094..8118)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8147..8268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8158..8177)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8212..8231
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8516..8533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8687..9275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8767..8786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9446..10306)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10069..10088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10307..10411)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10438..10893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10525..10544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10735..10756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    11024..11072
                     /note="synthetic polyadenylation signal"
     misc_feature    11086..11177
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     11126..11145
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcatca actttgtata gaaaagttgt agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctggt ttagtgaacc gtcagatcca agtttgtaca aaaaagcagg
      661 ctggaattcc atatgttcga aactgcagga attcccaccg ggacctgcgg ggctgagtgc
      721 ccttctcggt tgctgccgct gaggagcccg cccagccagc cagggccgcg aggccgaggc
      781 caggccgcag cccaggagcc gccccaccgc agctggcgat ggacccgccg aggcccgcgc
      841 tgctggcgct gctggcgctg cctgcgctgc tgctgctgct gctggcgggc gccagggccg
      901 aagaggaaat gctggaaaat gtcagcctgg tctgtccaaa agatgcgacc cgattcaagc
      961 acctccggaa gtacacatac aactatgagg ctgagagttc cagtggagtc cctgggactg
     1021 ctgattcaag aagtgccacc aggatcaact gcaaggttga gctggaggtt ccccagctct
     1081 gcagcttcat cctgaagacc agccagtgca ccctgaaaga ggtgtatggc ttcaaccctg
     1141 agggcaaagc cttgctgaag aaaaccaaga actctgagga gtttgctgca gccatgtcca
     1201 ggtatgagct caagctggcc attccagaag ggaagcaggt tttcctttac ccggagaaag
     1261 atgaacctac ttacatcctg aacatcaaga ggggcatcat ttctgccctc ctggttcccc
     1321 cagagacaga agaagccaag caagtgttgt ttctggatac cgtgtatgga aactgctcca
     1381 ctcactttac cgtcaagacg aggaagggca atgtggcaac agaaatatcc actgaaagag
     1441 acctggggca gtgtgatcgc ttcaagccca tccgcacagg catcagccca cttgctctca
     1501 tcaaaggcat gacccgcccc ttgtcaactc tgatcagcag cagccagtcc tgtcagtaca
     1561 cactggacgc taagaggaag catgtggcag aagccatctg caaggagcaa cacctcttcc
     1621 tgcctttctc ctacaagaat aagtatggga tggtagcaca agtgacacag actttgaaac
     1681 ttgaagacac accaaagatc aacagccgct tctttggtga aggtactaag aagatgggcc
     1741 tcgcatttga gagcaccaaa tccacatcac ctccaaagca ggccgaagct gttttgaaga
     1801 ctctccagga actgaaaaaa ctaaccatct ctgagcaaaa tatccagaga gctaatctct
     1861 tcaataagct ggttactgag ctgagaggcc tcagtgatga agcagtcaca tctctcttgc
     1921 cacagctgat tgaggtgtcc agccccatca ctttacaagc cttggttcag tgtggacagc
     1981 ctcagtgctc cactcacatc ctccagtggc tgaaacgtgt gcatgccaac ccccttctga
     2041 tagatgtggt cacctacctg gtggccctga tccccgagcc ctcagcacag cagctgcgag
     2101 agatcttcaa catggcgagg gatcagcgca gccgagccac cttgtatgcg ctgagccacg
     2161 cggtcaacaa ctatcataag acaaacccta cagggaccca ggagctgctg gacattgcta
     2221 attacctgat ggaacagatt caagatgact gcactgggga tgaagattac acctatttga
     2281 ttctgcgggt cattggaaat atgggccaaa ccatggagca gttaactcca gaactcaagt
     2341 cttcaatcct gaaatgtgtc caaagtacaa agccatcact gatgatccag aaagctgcca
     2401 tccaggctct gcggaaaatg gagcctaaag acaaggacca ggaggttctt cttcagactt
     2461 tccttgatga tgcttctccg ggagataagc gactggctgc ctatcttatg ttgatgagga
     2521 gtccttcaca ggcagatatt aacaaaattg tccaaattct accatgggaa cagaatgagc
     2581 aagtgaagaa ctttgtggct tcccatattg ccaatatctt gaactcagaa gaattggata
     2641 tccaagatct gaaaaagtta gtgaaagaag ctctgaaaga atctcaactt ccaactgtca
     2701 tggacttcag aaaattctct cggaactatc aactctacaa atctgtttct cttccatcac
     2761 ttgacccagc ctcagccaaa atagaaggga atcttatatt tgatccaaat aactaccttc
     2821 ctaaagaaag catgctgaaa actaccctca ctgcctttgg atttgcttca gctgacctca
     2881 tcgagattgg cttggaagga aaaggctttg agccaacatt ggaagctctt tttgggaagc
     2941 aaggattttt cccagacagt gtcaacaaag ctttgtactg ggttaatggt caagttcctg
     3001 atggtgtctc taaggtctta gtggaccact ttggctatac caaagatgat aaacatgagc
     3061 aggatatggt aaatggaata atgctcagtg ttgagaagct gattaaagat ttgaaatcca
     3121 aagaagtccc ggaagccaga gcctacctcc gcatcttggg agaggagctt ggttttgcca
     3181 gtctccatga cctccagctc ctgggaaagc tgcttctgat gggtgcccgc actctgcagg
     3241 ggatccccca gatgattgga gaggtcatca ggaagggctc aaagaatgac ttttttcttc
     3301 actacatctt catggagaat gcctttgaac tccccactgg agctggatta cagttgcaaa
     3361 tatcttcatc tggagtcatt gctcccggag ccaaggctgg agtaaaactg gaagtagcca
     3421 acatgcaggc tgaactggtg gcaaaaccct ccgtgtctgt ggagtttgtg acaaatatgg
     3481 gcatcatcat tccggacttc gctaggagtg gggtccagat gaacaccaac ttcttccacg
     3541 agtcgggtct ggaggctcat gttgccctaa aagctgggaa gctgaagttt atcattcctt
     3601 ccccaaagag accagtcaag ctgctcagtg gaggcaacac attacatttg gtctctacca
     3661 ccaaaacgga ggtgatccca cctctcattg agaacaggca gtcctggtca gtttgcaagc
     3721 aagtctttcc tggcctgaat tactgcacct caggcgctta ctccaacgcc agctccacag
     3781 actccgcctc ctactatccg ctgaccgggg acaccagatt agagctggaa ctgaggccta
     3841 caggagagat tgagcagtat tctgtcagcg caacctatga gctccagaga gaggacagag
     3901 ccttggtgga taccctgaag tttgtaactc aagcagaagg tgcgaagcag actgaggcta
     3961 ccatgacatt caaatataat cggcagagta tgaccttgtc cagtgaagtc caaattccgg
     4021 attttgatgt tgacctcgga acaatcctca gagttaatga tgaatctact gagggcaaaa
     4081 cgtcttacag actcaccctg gacattcaga acaagaaaat tactgaggtc gccctcatgg
     4141 gccacctaag ttgtgacaca aaggaagaaa gaaaaatcaa gggtgttatt tccatacccc
     4201 gtttgcaagc agaagccaga agtgagatcc tcgcccactg gtcgcctgcc aaactgcttc
     4261 tccaaatgga ctcatctgct acagcttatg gctccacagt ttccaagagg gtggcatggc
     4321 attatgatga agagaagatt gaatttgaat ggaacacagg caccaatgta gataccaaaa
     4381 aaatgacttc caatttccct gtggatctct ccgattatcc taagagcttg catatgtatg
     4441 ctaatagact cctggatcac agagtccctc aaacagacat gactttccgg cacgtgggtt
     4501 ccaaattaat agttgcaatg agctcatggc ttcagaaggc atctgggagt cttccttata
     4561 cccagacttt gcaagaccac ctcaatagcc tgaaggagtt caacctccag aacatgggat
     4621 tgccagactt ccacatccca gaaaacctct tcttaaaaag cgatggccgg gtcaaatata
     4681 ccttgaacaa gaacagtttg aaaattgaga ttcctttgcc ttttggtggc aaatcctcca
     4741 gagatctaaa gatgttagag actgttagga caccagccct ccacttcaag tctgtgggat
     4801 tccatctgcc atctcgagag ttccaagtcc ctacttttac cattcccaag ttgtatcaac
     4861 tgcaagtgcc tctcctgggt gttctagacc tctccacgaa tgtctacagc aacttgtaca
     4921 actggtccgc ctcctacagt ggtggcaaca ccagcacaga ccatttcagc cttcgggctc
     4981 gttaccacat gaaggctgac tctgtggttg acctgctttc ctacaatgtg caaggatctg
     5041 gagaaacaac atatgaccac aagaatacgt tcacactatc atatgatggg tctctacgcc
     5101 acaaatttct agattcgaat atcaaattca gtcatgtaga aaaacttgga aacaacccag
     5161 tctcaaaagg tttactaata ttcgatgcat ctagttcctg gggaccacag atgtctgctt
     5221 cagttcattt ggactccaaa aagaaacagc atttgtttgt caaagaagtc aagattgatg
     5281 ggcagttcag agtctcttcg ttctatgcta aaggcacata tggcctgtct tgtcagaggg
     5341 atcctaacac tggccggctc aatggagagt ccaacctgag gtttaactcc tcctacctcc
     5401 aaggcaccaa ccagataaca ggaagatatg aagatggaac cctctccctc acctccacct
     5461 ctgatctgca aagtggcatc attaaaaata ctgcttccct aaagtatgag aactacgagc
     5521 tgactttaaa atctgacacc aatgggaagt ataagaactt tgccacttct aacaagatgg
     5581 atatgacctt ctctaagcaa aatgcactgc tgcgttctga atatcaggct gattacgagt
     5641 cattgaggtt cttcagcctg ctttctggat cactaaattc ccatggtctt gagttaaatg
     5701 ctgacatctt aggcactgac aaaattaata gtggtgctca caaggcgaca ctaaggattg
     5761 gccaagatgg aatatctacc agtgcaacga ccaacttgaa gtgtagtctc ctggtgctgg
     5821 agaatgagct gaatgcagag cttggcctct ctggggcatc tatgaaatta acaacaaatg
     5881 gccgcttcag ggaacacaat gcaaaattca gtctggatgg gaaagccgcc ctcacagagc
     5941 tatcactggg aagtgcttat caggccatga ttctgggtgt cgacagcaaa aacattttca
     6001 acttcaaggt cagtcaagaa ggacttaagc tctcaaatga catgatgggc tcatatgctg
     6061 aaatgaaatt tgaccacaca aacagtctga acattgcagg cttatcactg gacttctctt
     6121 caaaacttga caacatttac agctctgaca agttttataa gcaaactgtt aatttacagc
     6181 tacagcccta ttctctggta actactttaa acagtgacct gaaatacaat gctctggatc
     6241 tcaccaacaa tgggaaacta cggctagaac ccctgaagct gcatgtggct ggtaacctaa
     6301 aaggagccta ccaaaataat gaaataaaac acatctatgc catctcttct gctgccttat
     6361 cagcaagcta taaagcagac actgttgcta aggttcaggg tgtggagttt agccatcggc
     6421 tcaacacaga catcgctggg ctggcttcag ccattgacat gagcacaaac tataattcag
     6481 actcactgca tttcagcaat gtcttccgtt ctgtaatggc cccgtttacc atgaccatcg
     6541 atgcacatac aaatggcaat gggaaactcg ctctctgggg agaacatact gggcagctgt
     6601 atagcaaatt cctgttgaaa gcagaacctc tggcatttac tttctctcat gattacaaag
     6661 gctccacaag tcatcatctc gtgtctagga aaagcatcag tgcagctctt gaacacaaag
     6721 tcagtgccct gcttactcca gctgagcaga caggcacctg gaaactcaag acccaattta
     6781 acaacaatga atacagccag gacttggatg cttacaacac taaagataaa attggcgtgg
     6841 agcttactgg acgaactctg gctgacctaa ctctactaga ctccccaatt aaagtgccac
     6901 ttttactcag tgagcccatc aatatcattg atgctttaga gatgagagat gccgttgaga
     6961 agccccaaga atttacaatt gttgcttttg taaagtatga taaaaaccaa gatgttcact
     7021 ccattaacct cccatttttt gagaccttgc aagaatattt tgagaggaat cgacaaacca
     7081 ttatagttgt actggaaaac gtacagagaa acctgaagca catcaatatt gatcaatttg
     7141 taagaaaata cagagcagcc ctgggaaaac tcccacagca agctaatgat tatctgaatt
     7201 cattcaattg ggagagacaa gtttcacatg ccaaggagaa actgactgct ctcacaaaaa
     7261 agtatagaat tacagaaaat gatatacaaa ttgcattaga tgatgccaaa atcaacttta
     7321 atgaaaaact atctcaactg cagacatata tgataggcac cagcggcggt tcaatggtga
     7381 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7441 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7501 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7561 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     7621 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     7681 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     7741 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7801 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     7861 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     7921 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     7981 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     8041 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaacccagc
     8101 tttcttgtac aaagtggtga tggccggccg cttcgagcag acatgataag atacattgat
     8161 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     8221 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     8281 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     8341 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     8401 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     8461 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     8521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9121 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10441 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    10501 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    10561 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    10621 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    10681 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    10741 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    10801 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    10861 cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact
    10921 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat
    10981 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt
    11041 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca
    11101 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg
    11161 tgccagaaca tttctctatc gata
//
