LOCUS Exported 10158 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of BRSK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics . ACCESSION . VERSION . KEYWORDS pHAGE-CMV-Flag-BRSK2_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10158) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 10158) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10158 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2227..2530 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2531..2734 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2685..2705 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2731..2755 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2826..2835 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2835..2858 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind 2868..2892 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4927..4951) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" intron 5025..5254 /label=chimeric intron /note="chimera between introns from adenovirus and immunoglobulin heavy chain genes" primer_bind 5237..5256 /label=pMT2-F /note="Synthetic intron, forward primer" misc_feature 5327..5900 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5494..5511) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5721..5740 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5933..6532 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5933..5952) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6429..6449 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6547..7135 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6600..6620) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7018..7029) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7210..7443 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7562..7580) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7648..7752 /gene="bla" /label=AmpR promoter CDS 7753..8613 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7971..7990) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8784..9372 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9273..9292 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9526..9543 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9636..9655 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9784..9805 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9820..9850 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9858..9874 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9863..9885 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9882..9898 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9882..9898 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9948..9967 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg 2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc 2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca 2881 aaaaagcaga agggccgtca aggcccacca tgacatcgac ggggaaggac ggcggcgcgc 2941 agcacgcgca gtatgttggg ccctaccggc tggagaagac gctgggcaag gggcagacag 3001 gtctggtgaa gctgggggtt cactgcgtca cctgccagaa ggtggccatc aagatcgtca 3061 accgtgagaa gctcagcgag tcggtgctga tgaaggtgga gcgggagatc gcgatcctga 3121 agctcattga gcacccccac gtcctaaagc tgcacgacgt ttatgaaaac aaaaaatatt 3181 tgtacctggt gctagaacac gtgtcaggtg gtgagctctt cgactacctg gtgaagaagg 3241 ggaggctgac gcctaaggag gctcggaagt tcttccggca gatcatctct gcgctggact 3301 tctgccacag ccactccata tgccacaggg atctgaaacc tgaaaacctc ctgctggacg 3361 agaagaacaa catccgcatc gcagactttg gcatggcgtc cctgcaggtt ggcgacagcc 3421 tgttggagac cagctgtggg tccccccact acgcctgccc cgaggtgatc cggggggaga 3481 agtatgacgg ccggaaggcg gacgtgtgga gctgcggcgt catcctgttc gccttgctgg 3541 tgggggctct gcccttcgac gatgacaact tgcgacagct gctggagaag gtgaagcggg 3601 gcgtgttcca catgccgcac tttatcccgc ccgactgcca gagtctgcta cggggcatga 3661 tcgaggtgga cgccgcacgc cgcctcacgc tagagcacat tcagaaacac atatggtata 3721 tagggggcaa gaatgagccc gaaccagagc agcccattcc tcgcaaggtg cagatccgct 3781 cgctgcccag cctggaggac atcgaccccg acgtgctgga cagcatgcac tcactgggct 3841 gcttccgaga ccgcaacaag ctgctgcagg acctgctgtc cgaggaggag aaccaggaga 3901 agatgattta cttcctcctc ctggaccgga aagaaaggta cccgagccag gaggatgagg 3961 acctgccccc ccggaacgag atagaccctc cccggaagcg tgtggactcc ccgatgctga 4021 accggcacgg caagcggcgg ccagaacgca aatccatgga ggtgctcagc gtgacggacg 4081 gcggctcccc ggtgcctgcg cggcgggcca ttgagatggc ccagcacggc cagaggtctc 4141 ggtccatcag cggtgcctcc tcaggccttt ccaccagccc actcagcagc ccccgggtga 4201 cccctcaccc ctcaccaagg ggcagtcccc tccccacccc caaggggaca cctgtccaca 4261 cgccaaagga gagcccggct ggcacgccca accccacgcc cccgtccagc cccagcgtcg 4321 gaggggtgcc ctggagggcg cggctcaact ccatcaagaa cagctttctg ggctcacccc 4381 gcttccaccg ccggaaactg caagttccga cgccggagga gatgtccaac ctgacaccag 4441 agtcgtcccc agagctggcg aagaagtcct ggtttgggaa cttcatcagc ctggagaagg 4501 aggagcagat cttcgtggtc atcaaagaca aacctctgag ctccatcaag gctgacatcg 4561 tgcacgcctt cctgtcgatt cccagtctca gccacagcgt catctcccaa acgagcttcc 4621 gggccgagta caaggccacg ggggggccag ccgtgttcca gaagccggtc aagttccagg 4681 ttgatatcac ctacacggag ggtggggagg cgcagaagga gaacggcatc tactccgtca 4741 ccttcaccct gctctcaggc cccagccgtc gcttcaagag ggtggtggag accatccagg 4801 cccagctgct gagcacacac gacccgcctg cggcccagca cttgtcagac accactaact 4861 gtatggaaat gatgacgggg cggctttcca aatgtggaat tatcccgaaa agttcaggcc 4921 tcatgggccc agctttcttg tacaaagtgg ttgattaaga tccactagta acggccgcca 4981 gtgtgctgga attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc 5041 aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat 5101 attcacctgg cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac 5161 aatctttttg ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt 5221 gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag 5281 gtcgagcatg catctagggc ggccaattcc gcccctctcc cccccccccc tctccctccc 5341 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 5401 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 5461 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 5521 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 5581 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 5641 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 5701 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 5761 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 5821 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 5881 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga 5941 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 6001 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 6061 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 6121 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 6181 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 6241 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 6301 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 6361 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 6421 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 6481 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatatcgata 6541 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 6961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 7081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag 7141 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 7201 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 7261 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 7321 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 7381 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 7441 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 7501 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag 7561 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 7621 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 7681 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 7741 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 7801 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 7861 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 7921 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 7981 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct 8041 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 8101 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 8161 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 8221 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 8281 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 8341 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 8401 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 8461 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 8521 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 8581 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 8641 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 8701 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 8761 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 8821 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 8881 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 8941 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 9001 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 9061 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 9121 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 9181 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 9241 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 9301 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 9361 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 9421 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 9481 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 9541 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 9601 taatgcagca agctcatggc tgactaattt ttttttattt atgcagaggc cgaggccgcc 9661 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 9721 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 9781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 9841 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat 9901 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 9961 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 10021 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 10081 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 10141 tactacaaac ttagtagt //