LOCUS       Exported                 898 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with H134R mutation 
            fused to EYFP driven by CaMKIIa promoter for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tnnnnngnnn gncccggnan gctctgccct gctcacggcg gccgctggca cggctccggc
       61 ctcggcttcg tcttcgacga gagtctcgac ctcgatctcc gttccgccga tgttcagttt
      121 ggtggttttg cggatatctc cgtgaatcaa tatgtgctcg tggatcagga cgcgcaggta
      181 gtgtcccaac aacccccaac aatttttact catcagatca ataatcgtgt gacctacggt
      241 ggagccatag acgctcagga cgccaaaacc ttcgggcccc aaaatgaaga gaattgggaa
      301 cataccccag ctcacgaaaa acagccatgc catgccggtc acgacctggc ggcaccgacc
      361 ctttggcaca gtatgataac cctcgatata tgctttggcg gcgtgaaaaa atgtgttcgc
      421 gccatagcac aatccaagac aaaagaagat gactttaaca tagccggttg ccatggcgct
      481 ggtagccccc cacacgatag tcccgatgtc tgagacaagg agtcccatgg ttctcctgct
      541 gtagtcgttg ctcaggccgg tgaggttgct caggcggata aggatgacag gacaagtgag
      601 cagccactct gcatagcgca gccactgcac ccggtgtcct gtggcaaggt agagcataga
      661 gggattctta aactcaaaaa agaactcgag aatcacctta accatttcaa tggcgcacac
      721 atagatctcc tcccagccgc atgtagattt ccaggtttgg taggcataga acatcagcag
      781 caaatgctga atcctgctgc agcactgcag gaatttgagc gtctgagcgc gtcgtgcgcg
      841 agatcatcat cggaatacat gatcnaggac agangacgtc cnccagatat acacaatc
//
