LOCUS Exported 13454 bp ds-DNA circular SYN 01-JUN-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV delta R8.2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13454) TITLE Trono Lab Packaging and Envelope Plasmids REFERENCE 2 (bases 1 to 13454) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 1, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13454 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" CDS 855..2357 /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /label=HIV-1 gag /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" CDS 2150..5161 /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /label=HIV-1 pol /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" misc_feature 4846..4963 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 6532..6765 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 6950..6994 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" CDS 7288..7314 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" promoter complement(8551..8569) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8552..8569) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(8859..8878) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(9092..9111) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9211..9233 /label=pGEX 3' /note="pGEX vectors, reverse primer" promoter 9357..9461 /gene="bla" /label=AmpR promoter CDS 9462..10322 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9680..9699) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10493..11081 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10982..11001 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11235..11252 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(11322..11342) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 11327..11656 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 11507..11642 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11569..11588 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 12790..12855 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 12985..13005 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg 721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgcg acggatcgat 781 cccgtaataa gcttcgaggt ccgcggccgc gttgacgcgc acggcaagag gcgaggggcg 841 gcgactggtg agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgat 901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 1021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac 1201 agcaagcagc agctgacaca ggacacagca atcaggtcag ccaaaattac cctatagtgc 1261 agaacatcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg 1321 taaaagtagt agaagagaag gctttcagcc cagaagtgat acccatgttt tcagcattat 1381 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatcaag 1441 cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagagtgc 1501 atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca 1561 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta 1621 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa 1681 tgtatagccc taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact 1741 atgtagaccg attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt 1801 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag 1861 cattgggacc aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac 1921 ccggccataa agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca 1981 taatgataca gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg 2041 gcaaagaagg gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat 2101 gtggaaagga aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga 2161 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa 2221 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc 2281 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg 2341 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc 2401 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg 2461 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg 2521 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa 2581 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc 2641 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga 2701 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa 2761 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac 2821 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga 2881 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact 2941 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc 3001 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct 3061 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga 3121 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt 3181 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct 3241 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg 3301 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa 3361 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca 3421 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact 3481 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat 3541 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat 3601 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct 3661 gaaaacagga aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac 3721 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt 3781 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac 3841 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt 3901 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga 3961 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct 4021 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc 4081 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca 4141 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga 4201 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga 4261 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca 4321 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc 4381 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc 4441 catgcatgga caagtagact gtagcccagg aatatggcag ctagattgta cacatttaga 4501 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat 4561 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc 4621 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc 4681 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg 4741 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc 4801 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg 4861 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 4921 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 4981 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt 5041 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag 5101 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta 5161 acacatggaa aagattagta aaacaccata tgtatatttc aaggaaagct aaggactggt 5221 tttatagaca tcactatgaa agtactaatc caaaaataag ttcagaagta cacatcccac 5281 taggggatgc taaattagta ataacaacat attggggtct gcatacagga gaaagagact 5341 ggcatttggg tcagggagtc tccatagaat ggaggaaaaa gagatatagc acacaagtag 5401 accctgacct agcagaccaa ctaattcatc tgcactattt tgattgtttt tcagaatctg 5461 ctataagaaa taccatatta ggacgtatag ttagtcctag gtgtgaatat caagcaggac 5521 ataacaaggt aggatctcta cagtacttgg cactagcagc attaataaaa ccaaaacaga 5581 taaagccacc tttgcctagt gttaggaaac tgacagagga cagatggaac aagccccaga 5641 agaccaaggg ccacagaggg agccatacaa tgaatggaca ctagagcttt tagaggaact 5701 taagagtgaa gctgttagac attttcctag gatatggctc cataacttag gacaacatat 5761 ctatgaaact tacggggata cttgggcagg agtggaagcc ataataagaa ttctgcaaca 5821 actgctgttt atccatttca gaattgggtg tcgacatagc agaataggcg ttactcgaca 5881 gaggagagca agaaatggag ccagtagatc ctagactaga gccctggaag catccaggaa 5941 gtcagcctaa aactgcttgt accaattgct attgtaaaaa gtgttgcttt cattgccaag 6001 tttgtttcat gacaaaagcc ttaggcatct cctatggcag gaagaagcgg agacagcgac 6061 gaagagctca tcagaacagt cagactcatc aagcttctct atcaaagcag taagtagtac 6121 atgtaatgca acctataata gtagcaatag tagcattagt agtagcaata ataatagcaa 6181 tagttgtgtg gtccatagta atcatagaat ataggaaaat attaagacaa agaaaaatag 6241 acaggttaat tgatagacta atagaaagag cagaagacag tggcaatgag agtgaaggag 6301 aagtatcagc acttgtggag atgggggtgg aaatggggca ccatgctcct tgggatattg 6361 atgatctgta gttgagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa 6421 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 6481 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 6541 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 6601 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 6661 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 6721 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 6781 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 6841 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta 6901 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca 6961 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg 7021 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt 7081 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca 7141 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg 7201 agagagagac agagacagat ccattcgatt agtgaacgga tccttggcac ttatctggga 7261 cgatctgcgg agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt 7321 aacgaggatt gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa 7381 tctcctacaa tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac 7441 agccatagca gtagctgagg ggacagatag ggttatagaa gtagtacaag gagcttgtag 7501 agctattcgc cacataccta gaagaataag acagggcttg gaaaggattt tgctataaga 7561 tgggtggcaa gtggtcaaaa agtagtgtga ttggatggcc tactgtaagg gaaagaatga 7621 gacgagctga gccagcagca gatggggtgg gagcagtatc tcgagaccta gaaaaacatg 7681 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 7741 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 7801 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 7861 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 7921 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat 7981 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga 8041 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat 8101 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg 8161 agtacttcaa gaactgctga gcggccgccc cggtgacctt cagaccttgg cactggaggt 8221 ggcccggcag aagcgcggca tcgtggatca gtgctgcacc agcatctgct ctctctacca 8281 actggagaac tactgcaact aggcccacca ctaccctgtc cacccctctg caatgaataa 8341 aacctttgaa agagcactac aagttgtgtg tacatgcgtg catgtgcata tgtggtgcgg 8401 ggggaacatg agtggggctg gctggagtgg cgatgataag ctgtcaaaca tgagaattct 8461 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 8521 gtttcttagt ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat 8581 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa 8641 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag 8701 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat 8761 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt 8821 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga 8881 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt 8941 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc 9001 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca 9061 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 9121 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 9181 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 9241 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 9301 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 9361 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 9421 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 9481 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 9541 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 9601 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 9661 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 9721 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 9781 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 9841 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 9901 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 9961 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 10021 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 10081 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 10141 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 10201 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 10261 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 10321 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 10381 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 10441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 10501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 10561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 10621 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 10681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 10741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 10801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 10861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 10921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 10981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 11041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 11101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 11161 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 11221 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 11281 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg 11341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 11401 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 11461 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 11521 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 11581 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 11641 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac 11701 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac 11761 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt 11821 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct 11881 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat 11941 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga 12001 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg 12061 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga 12121 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt 12181 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat 12241 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat 12301 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag 12361 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc 12421 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc 12481 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc 12541 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa 12601 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat 12661 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt 12721 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa 12781 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt 12841 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc 12901 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact 12961 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac 13021 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct 13081 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa 13141 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt 13201 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc 13261 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact 13321 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 13381 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgttg ggctgcagga 13441 attcgagctc gccc //