LOCUS Exported 6653 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Cytoskeleton, Excitation: 554, Emission: 581. ACCESSION . VERSION . KEYWORDS tdTomato-Myotilin-C-14 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6653) TITLE Davidson Orange Fluorescent Proteins REFERENCE 2 (bases 1 to 6653) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6653 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 125..328 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 279..299 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 367..376 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 373..1800 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" polyA_signal 3466..3587 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3503..3522) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3557..3576 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3594..4049) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3731..3752) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3943..3962 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4076..4180 /gene="bla" /label=AmpR promoter primer_bind complement(4205..4225) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4249..4459 /label=SV40 promoter /note="SV40 early promoter" promoter 4271..4467 /label=SV40 promoter /note="SV40 early promoter" rep_origin 4318..4453 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4380..4399 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4502..5296 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4556..4575) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5166..5185 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5484..5503) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5528..5575 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5904..6492 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6393..6412 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggcatt atgcccagta 61 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 121 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 181 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 241 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 301 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 361 ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag 421 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc 481 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 541 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac 601 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc 661 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac 721 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta 781 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 841 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg 901 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg 961 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 1021 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc 1081 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc 1141 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc 1201 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc 1261 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg 1321 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg 1381 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg 1441 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc 1501 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 1561 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac 1621 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac 1681 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag 1741 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag 1801 tccggaagtg ctgggagcgc aagcggaagc ggaggctccg gttttaacta cgaacgtcca 1861 aaacacttca tccagtccca aaacccatgt ggctccagat tgcagcctcc tggaccagaa 1921 acctccagct tctctagcca gaccaaacag tcttccatta tcatccagcc ccgccagtgt 1981 acagagcaaa gattttctgc ctcctcaaca ctgagctctc acatcaccat gtcctcctct 2041 gctttccctg cttctcccca gcagcatgct ggctccaacc caggccaaag ggttacaacc 2101 acctataacc agtccccagc cagcttcctc agctccatat taccatcaca gcctgattac 2161 aatagcagta aaatcccttc cgctatggat tccaactatc aacagtcctc agctggccaa 2221 cctataaatg caaagccatc ccaaactgca aatgctaagc ccataccaag aactcctgat 2281 catgaaatac aaggatcaaa agaagctttg attcaagatt tggaaagaaa gctgaaatgc 2341 aaggacaccc ttcttcataa tggaaatcaa cgtctaacat atgaagagaa gatggctcgc 2401 agattgctag gaccacagaa tgcagctgct gtgtttcaag ctcaggatga cagtggtgca 2461 caagactcgc agcaacacaa ctcagaacat gcgcgactgc aagttcctac atcacaagta 2521 agaagtagat caacctcaag gggagatgtg aatgatcagg atgcaatcca ggagaaattt 2581 tacccaccac gtttcattca agtgccagag aacatgtcga ttgatgaagg aagattctgc 2641 agaatggact tcaaagtgag tggactgcca gctcctgatg tgtcatggta tctaaatgga 2701 agaacagttc aatcagatga tttgcacaaa atgatagtgt ctgagaaggg tcttcattca 2761 ctcatctttg aagtagtcag agcttcagat gcaggggctt atgcatgtgt tgccaagaat 2821 agagcaggag aagccacctt cactgtgcag ctggatgtcc ttgcaaaaga acataaaaga 2881 gcaccaatgt ttatctacaa accacagagc aaaaaagttt tagagggaga ttcagtgaaa 2941 ctagaatgcc agatctcggc tatacctcca ccaaagcttt tctggaaaag aaataatgaa 3001 atggtacaat tcaacactga ccgaataagc ttatatcaag ataacactgg aagagttact 3061 ttactgataa aagatgtaaa caagaaagat gctgggtggt atactgtgtc agcagttaat 3121 gaagctggag tgactacatg taacacaaga ttagacgtta cggcacgtcc aaaccaaact 3181 cttccagctc ctaagcagtt acgggttcga ccaacattca gcaaatattt agcacttaat 3241 gggaaaggtt tgaatgtaaa acaagctttt aacccagaag gagaatttca gcgtttggca 3301 gctcaatctg gactctatga aagtgaagaa ctttaaggat ccaccggatc tagataactg 3361 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 3421 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 3481 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 3541 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt 3601 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 3661 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 3721 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 3781 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 3841 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 3901 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 3961 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 4021 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 4081 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 4141 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag 4201 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 4261 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 4321 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 4381 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 4441 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 4501 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 4561 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 4621 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 4681 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 4741 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 4801 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4861 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4921 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4981 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 5041 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 5101 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 5161 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 5221 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 5281 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 5341 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 5401 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 5461 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 5521 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 5581 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 5641 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 5701 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 5761 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 5821 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 5881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 5941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 6061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 6481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 6541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 6601 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc cat //