LOCUS Exported 5934 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pGL2-Mcl-1 mut SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5934) AUTHORS Ricci MS, Kim SH, Ogi K, Plastaras JP, Ling J, Wang W, Jin Z, Liu YY, Dicker DT, Chiao PJ, Flaherty KT, Smith CD, El-Deiry WS TITLE Reduction of TRAIL-induced Mcl-1 and cIAP2 by c-Myc or sorafenib sensitizes resistant human cancer cells to TRAIL-induced death. JOURNAL Cancer Cell. 2007 Jul . 12(1):66-80. PUBMED 17613437 REFERENCE 2 (bases 1 to 5934) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5934 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 432..2084 /codon_start=1 /gene="luc" /product="firefly luciferase" /label=luciferase /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(498..516) /label=LucNrev /note="Firefly luciferase, reverse primer" intron 2325..2390 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2520..2540 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 2965..3099 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3241..3258) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3412..4000) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3492..3511) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4171..5031) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4794..4813 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5032..5136) /gene="bla" /label=AmpR promoter rep_origin 5163..5618 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5250..5269) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5460..5481 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 5808..5929 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 ctgtaactga gctaacataa cccgggaggt accgagctct tacgcgtgct agctcgagcc 61 caggagctct gggaggaccc caggcgctcg gagccgccgt tacgtaaccg gcactcagag 121 cctccgaaga ccggaaggcc ccgctcaggc cccggccccg gccccggccc cgccccggcc 181 cggccgggca gctggtaggt gccgtgcgca accctccgga agctgccgcc cctttcccct 241 tttatgggaa tacttttttt aaaaaaaaag agttcgctgg cgccaccccg taggactggc 301 cgccctaaaa ccgtgataaa ggagctgctc gccacttctc acttccgctt ccttccagta 361 aggagtcgtt gtcttcccca gttttctcag ccaggcggcg gcaagcttgg cattccggta 421 ctgttggtaa aatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc 481 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc 541 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact 601 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca 661 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat 721 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca 781 gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt 841 tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg 901 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta 961 atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga 1021 attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg 1081 tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga 1141 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga 1201 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc 1261 ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca 1321 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg 1381 cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac 1441 gacaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg 1501 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg 1561 ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga 1621 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat 1681 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atagttgacc 1741 gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa ttggaatcga 1801 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg 1861 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag 1921 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg 1981 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag 2041 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca 2101 gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg gggcgtaatt 2161 tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa 2221 taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg 2281 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt 2341 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct 2401 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct 2461 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc 2521 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt 2581 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg 2641 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta 2701 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt 2761 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg 2821 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca 2881 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 2941 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3061 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtcgaccgat gcccttgaga 3121 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 3181 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc 3241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 3361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 3601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 3961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 4081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 4201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 4321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 4381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 4441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 4501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 4561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 4621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 4681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 4741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 4801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 4861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 4921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 4981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 5041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 5101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 5161 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 5581 taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg 5641 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc 5701 atgataagta agtaatatta aggtacgtgg aggttttact tgctttaaaa aacctcccac 5761 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 5821 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5881 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat ggta //