LOCUS Exported 6903 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pClneoMyc human NDR1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6903) AUTHORS Hirabayashi S, Nakagawa K, Sumita K, Hidaka S, Kawai T, Ikeda M, Kawata A, Ohno K, Hata Y TITLE Threonine 74 of MOB1 is a putative key phosphorylation site by MST2 to form the scaffold to activate nuclear Dbf2-related kinase 1. JOURNAL Oncogene. 2008 Jul 17;27(31):4281-92. Epub 2008 Mar 24. PUBMED 18362890 REFERENCE 2 (bases 1 to 6903) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6903 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 22..401 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 402..613 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 564..584 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 610..634 /label=LNCX /note="Human CMV promoter, forward primer" intron 774..906 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 889..908 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 951..969 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 987..1016 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter complement(2456..2473) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 2491..2612 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2502..2521) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2556..2575 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 2798..3253 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2885..2904) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3095..3116 /label=F1ori-F /note="F1 origin, forward primer" promoter 3370..3727 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3393..3413) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3578..3713 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3640..3659 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3778..4572 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3832..3851) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4442..4461 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4636..4684 /note="synthetic polyadenylation signal" primer_bind complement(4725..4744) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4844..4866 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4904..4922) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4990..5094 /gene="bla" /label=AmpR promoter CDS 5095..5955 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5313..5332) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6126..6714 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6615..6634 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gtccaatatg accgccatgt tggcattgat tattgactag ttattaatag taatcaatta 61 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 241 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca 301 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta 361 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 421 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 481 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 541 actgcgatcg cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 601 tataagcaga gctcgtttag tgaaccgtca gatcactaga agctttattg cggtagttta 661 tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg 721 cagtgactct cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta 781 tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa 841 gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt gcctttctct 901 ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact 961 cactataggc tagacccccc aacatggagc agaagcttat cagcgaggag gacctgctcg 1021 aggaattcac gcgtgccatg gcaatgacag gctcaacacc ttgctcatcc atgagtaacc 1081 acacaaagga aagggtgaca atgaccaaag tgacactgga gaatttttat agcaacctta 1141 ttgctcaaca tgaagaacga gaaatgagac aaaagaagtt agaaaaggtg atggaagaag 1201 aaggcctaaa agatgaggag aaacgactcc ggagatcagc acatgctcgg aaggaaacag 1261 agtttcttcg tttgaagaga acaagacttg gattggaaga ttttgagtcc ttaaaagtaa 1321 taggcagagg agcatttggt gaggtacggc ttgttcagaa gaaagatacg ggacatgtgt 1381 atgcaatgaa aatactccgt aaagcagata tgcttgaaaa agagcaggtt ggccacattc 1441 gtgcggagcg tgacattcta gtggaggcag acagtttgtg ggttgtgaaa atgttctata 1501 gttttcagga taagctaaac ctctacctaa tcatggagtt cctgcctgga ggggacatga 1561 tgaccttgtt gatgaaaaaa gacactctga cagaagagga gactcagttt tatatagcag 1621 aaacagtatt agccatagac tctattcacc aacttggatt catccacaga gacatcaaac 1681 cagacaacct tcttttggac agcaagggcc atgtgaaact ttctgacttt ggtctttgca 1741 caggactgaa aaaagcacat aggacagaat tttataggaa tctgaaccac agcctcccca 1801 gtgatttcac tttccagaac atgaattcca aaaggaaagc agaaacctgg aaaagaaata 1861 gacgtcagct agccttctcc acagtaggca ctcctgacta cattgctcct gaggtgttca 1921 tgcagaccgg gtacaacaag ctctgtgatt ggtggtcgct tggggtgatc atgtatgaga 1981 tgctcatcgg ctacccacct ttctgttctg agacccctca agagacatat aagaaggtga 2041 tgaactggaa agaaactttg acttttcctc cagaagttcc catctctgag aaagccaagg 2101 atctaatttt gaggttctgc tgtgaatggg aacatagaat tggagctcct ggagttgagg 2161 aaataaaaag taactctttt tttgaaggcg ttgactggga acatatcaga gagagacctg 2221 ctgcaatatc tattgaaatc aaaagcattg atgatacctc aaacttcgat gagtctccag 2281 aatctgatat tcttaagcca acagtggcca caagtaatca tcctgagact gactacaaga 2341 acaaagactg ggtcttcatc aattacacgt acaagcgctt tgagggcctg actgcaaggg 2401 gggcaatacc ttcctacatg aaagcagcaa aatagtcgac ccgggcggcc gcttcccttt 2461 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca 2521 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 2581 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 2641 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 2701 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg 2761 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta 2821 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 2881 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 2941 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3001 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3061 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3121 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 3181 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 3241 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 3301 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt 3361 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 3421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3481 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 3541 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 3601 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 3661 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 3721 ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg 3781 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 3841 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3901 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 3961 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 4021 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 4081 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 4141 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 4201 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 4261 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 4321 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 4381 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 4441 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 4501 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 4561 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 4621 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg 4681 tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat 4741 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 4801 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 4861 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 4921 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 4981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 5041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 5101 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 5161 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5221 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5281 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 5341 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5401 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5461 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5521 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5581 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 5641 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 5701 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 5761 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 5821 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 5881 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 5941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6241 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 6301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6481 acgacctaca ccgaactgag atacctacag cgtgagctat gacaaagcgc cacgcttccc 6541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga 6781 cagatcttca atattggcca ttagccatat tattcattgg ttatatagca taaatcaata 6841 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct 6901 cat //