LOCUS       Exported                5658 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Yellow protein calcium sensor expressed under human Synapsin1 
            promoter, for AAV production or direct transfection.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-syn-jYCaMP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Mohr MA, Bushey D, Aggarwal A, Marvin JS, Kim JJ, Marquez EJ, Liang 
            Y, Patel R, Macklin JJ, Lee CY, Tsang A, Tsegaye G, Ahrens AM, Chen 
            JL, Kim DS, Wong AM, Looger LL, Schreiter ER, Podgorski K
  TITLE     jYCaMP: an optimized calcium indicator for two-photon imaging at 
            fiber laser wavelengths.
  JOURNAL   Nat Methods. 2020 May 25. pii: 10.1038/s41592-020-0835-7. doi: 
            10.1038/s41592-020-0835-7.
  PUBMED    32451475
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        212..659
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          681..813
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     796..815
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      827..836
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             848..904
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     1117..1138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1244..1265)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1505..1524)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2096..2684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2149..2169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2567..2578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2687..2703)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2688..2704)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2744..2763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2798..2817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3016..3145
                     /label=AAV2 ITR
     rep_origin      3217..3672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3304..3323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3514..3535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3698..3802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3803..4663
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4021..4040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4834..5422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5323..5342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5576..5593
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatct ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga
      241 ggcggggtgg gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc
      301 ccattcccca aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag
      361 gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg
      421 cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa
      481 ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc
      541 acgcgaggcg cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact
      601 cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt
      661 cgaattcaag ctgctagcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
      721 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
      781 ttactgacat ccactttgcc tttctctcca caggtgataa ggatccgcca ccatggtcga
      841 ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag
      901 ctcactcgag aacgtctata tcaccgccga caagcagaag aacggcatca aggcgaactt
      961 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa
     1021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagcc
     1081 cgtgctttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1141 cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat
     1201 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1261 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg
     1321 caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1381 cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
     1441 gcacgacttc ttcaagtccg ccatgcccga aggctacgtg caggagcgca ccatcttctt
     1501 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1561 gaaccgcatc gagcttaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1621 gctggagtac aacccacctg accaactgac tgaagagcag atcgcagaat ttaaagagct
     1681 tttctcccta tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt
     1741 gatgcggtct ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt
     1801 agatgccgac ggtgacggca caatcgactt ccctgagttc ctgacaatgt acgcaagaaa
     1861 aatgaaatac agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga
     1921 tggcaatggc tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa
     1981 gttaacagat gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca
     2041 ggtaaactac gaagagtttg tacaaatgat gacagcgaag tagaagctta tcgataatca
     2101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2461 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     2701 tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     2761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2881 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2941 tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt
     3001 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3061 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3121 cctcagtgag cgagcgagcg cgcagctggc gtaatagcga agaggcccgc accgatcgcc
     3181 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     3241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3421 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3541 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3661 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3781 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3841 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3901 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3961 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4021 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4081 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4141 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4201 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4261 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4321 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4381 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4441 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4501 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4561 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4621 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4681 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4981 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5521 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5581 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5641 gccgattcat taatgcag
//
