LOCUS Exported 6861 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV donor/transfer vector expressing CreLite system components, PhyB-Delta-CreC and PIF6CreN, driven by CBh promoter. ACCESSION . VERSION . KEYWORDS pAAV-CreLite SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6861) AUTHORS Yen ST, Trimmer KA, Aboul N, Mullen RD, Culver JC, Dickinson ME, Behringer RR, Eisenhoffer GT TITLE CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light JOURNAL bioRxiv REFERENCE 2 (bases 1 to 6861) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6861 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR CDS 593..613 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3443..3499 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 3503..3523 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 4117..4165 /note="synthetic polyadenylation signal" repeat_region 4219..4348 /label=AAV2 ITR rep_origin 4420..4875 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4507..4526) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4717..4738 /label=F1ori-F /note="F1 origin, forward primer" promoter 4901..5005 /gene="bla" /label=AmpR promoter CDS 5006..5866 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5224..5243) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6037..6625 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6526..6545 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6779..6796 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 ggaaagcttc gcccttaagc tagtatgcca agtacgcccc ctattgacgt caatgacggt 241 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 301 tacatctact cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc 361 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg aggggcgggg 421 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 481 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 541 agcgggatca gaatgatctg atatcatcga tgaattcgag ctcgccacca tgcccaagaa 601 gaagaggaag gtggtttccg gagtcggggg tagtggcggt ggccgtggcg gtggccgtgg 661 cggagaagaa gaaccgtcgt caagtcacac tcctaataac cgaagaggag gagaacaagc 721 tcaatcgtcg ggaacgaaat ctctcagacc aagaagcaac actgaatcaa tgagcaaagc 781 aattcaacag tacaccgtcg acgcaagact ccacgccgtt ttcgaacaat ccggcgaatc 841 agggaaatca ttcgactact cacaatcact caaaacgacg acgtacggtt cctctgtacc 901 tgagcaacag atcacagctt atctctctcg aatccagcga ggtggttaca ttcagccttt 961 cggatgtatg atcgccgtcg atgaatccag tttccggatc atcggttaca gtgaaaacgc 1021 cagagaaatg ttagggatta tgcctcaatc tgttcctact cttgagaaac ctgagattct 1081 agctatggga actgatgtga gatctttgtt cacttcttcc agctcgattc tactcgagcg 1141 tgctttcgtt gctcgagaga ttaccttgtt aaatccggtt tggatccatt ccaagaatac 1201 tggtaaaccg ttttacgcca ttcttcatag gattgatgtt ggtgttgtta ttgatttaga 1261 gccagctaga actgaagatc ctgcgctttc tattgctggt gctgttcaat cgcagaaact 1321 cgcggttcgt gcgatttctc agttacaggc tcttcctggt ggagatatta agctgttgtg 1381 tgacactgtc gtggaaagtg tgagggactt gactggttat gatcgtgtta tggtttataa 1441 gtttcatgaa gatgagcatg gagaagttgt agctgagagt aaacgagatg atttagagcc 1501 ttatattgga ctgcattatc ctgctactga tattcctcaa gcgtcaaggt tcttgtttaa 1561 gcagaaccgt gtccgaatga tagtagattg caatgccaca cctgttcttg tggtccagga 1621 cgataggcta actcagtcta tgtgcttggt tggttctact cttagggctc ctcatggttg 1681 tcactctcag tatatggcta acatgggatc tattgcgtct ttagcaatgg cggttataat 1741 caatggaaat gaagatgatg ggagcaatgt agctagtgga agaagctcga tgaggctttg 1801 gggtttggtt gtttgccatc acacttcttc tcgctgcata ccgtttccgc taaggtatgc 1861 ttgtgagttt ttgatgcagg ctttcggttt acagttaaac atggaattgc agttagcttt 1921 gcaaatgtca gagaaacgtg ttttgagaac gcagacactg ttatgtgata tgcttctgcg 1981 tgactcgcct gctggaattg ttacacagag tcccagtatc atggacttag tgaaatgtga 2041 cggtgcagca tttctttacc acgggaagta ttacccgttg ggtgttgctc ctagtgaagt 2101 tcagataaaa gatgttgtgg agtggttgct tgcgaatcat gcggattcaa ccggattaag 2161 cactgatagt ttaggcgatg cggggtatcc cggtgcagct gcgttagggg atgctgtgtg 2221 cggtatggca gttgcatata tcacaaaaag agactttctt ttttggtttc gatctcacac 2281 tgcgaaagaa atcaaatggg gaggcgctaa gcatcatccg gaggataaag atgatgggca 2341 acgaatgcat cctcgttcgt cctttcaggc ttttcttgaa gttgttaaga gccggagtca 2401 gccatgggaa actgcggaaa tggatgcgat tcactcgctc cagcttattc tgagagactc 2461 ttttaaagaa tctggtggtt ctgccggtgg ctccggttct ggctccagcg gtggcagctc 2521 tggtgcgtcc ggcacgggta ctgcgggtgg cactggcagc ggttccggta ctggctctgg 2581 caaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca 2641 ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca 2701 tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg 2761 gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg 2821 cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg 2881 taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag 2941 gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag 3001 aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa 3061 actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt 3121 ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg 3181 cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc 3241 tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat 3301 ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa 3361 tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct 3421 ggaagatggc gatggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt 3481 ggaggagaac cctggaccta tgcccaagaa gaagaggaag gtgatgttct taccaaccga 3541 ttattgttgc aggttaagcg atcaagagta tatggagctt gtgtttgaga atggccagat 3601 tcttgcaaag ggccaaagat ccaacgtttc tctgcataat caacgtacca aatcgatcat 3661 ggatttgtat gaggcagagt ataacgagga tttcatgaag agtatcatcc atggtggtgg 3721 tggtgccatc acaaatctcg gggacacgca ggttgttcca caaagtcatg ttgctgctgc 3781 ccatgaaaca aacatgttgg aaagcaataa acatgttgac ggtggttctg ccggtggctc 3841 cggttctggc tccagcggtg gcagctctgg tgcgtccggc acgggtactg cgggtggcac 3901 tggcagcggt tccggtactg gctctggcct gactgtgcac caaaacctgc ctgccctccc 3961 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca 4021 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg 4081 gtgcaagctg aactgagaat tctctagact agagcgaata aaagatcttt attttcatta 4141 gatctgtgtg ttggtttttt gtgtgatgca gcacgcgtta cgtagataag tagcatggcg 4201 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg 4261 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg 4321 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc 4381 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 4441 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 4501 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 4561 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 4621 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 4681 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 4741 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 4801 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 4861 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 4921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 4981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 5041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 5101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 5161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 5221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 5281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 5341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 5401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 5461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 5521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 5581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 5641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 5701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 5761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 5821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 6181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 6661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 6721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 6781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 6841 ttggccgatt cattaatgca g //