LOCUS Exported 6956 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian, Zebrafish, or Xenopus expression of PhyB-Delta-CreC, one half of the CreLite system.. ACCESSION . VERSION . KEYWORDS pCS2+PhyB-Delta-CreC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6956) AUTHORS Yen ST, Trimmer KA, Aboul N, Mullen RD, Culver JC, Dickinson ME, Behringer RR, Eisenhoffer GT TITLE CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light JOURNAL bioRxiv REFERENCE 2 (bases 1 to 6956) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6956 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 845..863 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 845..862 /label=SP6 /note="SP6 promoter, forward primer" CDS 934..954 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter complement(3796..3813) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 3818..3952 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3842..3861) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3896..3915 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(4065..4085) /label=T3 /note="T3 promoter, forward primer" promoter complement(4065..4083) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4104..4120) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4104..4120) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4117..4139) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4128..4144 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4152..4182) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4197..4218 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4335..4352) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4506..5094) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4586..4605) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5265..6125) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5888..5907 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6126..6230) /gene="bla" /label=AmpR promoter rep_origin complement(6256..6711) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6393..6414) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6605..6624 /label=F1ori-R /note="F1 origin, reverse primer" promoter 6789..810 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 acctggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg 61 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca 121 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat 181 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg 241 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg 301 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc 361 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg 421 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac 481 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga 541 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg 601 caagtacatt actattggaa gtacgccagg gtacattggc agtactccca ttgacgtcaa 661 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg 721 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag 781 gtctatataa gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag 841 cttgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga 901 ttcgaattcc tcgaggatat cgccgccacc atgcccaaga agaagaggaa ggtggtttcc 961 ggagtcgggg gtagtggcgg tggccgtggc ggtggccgtg gcggagaaga agaaccgtcg 1021 tcaagtcaca ctcctaataa ccgaagagga ggagaacaag ctcaatcgtc gggaacgaaa 1081 tctctcagac caagaagcaa cactgaatca atgagcaaag caattcaaca gtacaccgtc 1141 gacgcaagac tccacgccgt tttcgaacaa tccggcgaat cagggaaatc attcgactac 1201 tcacaatcac tcaaaacgac gacgtacggt tcctctgtac ctgagcaaca gatcacagct 1261 tatctctctc gaatccagcg aggtggttac attcagcctt tcggatgtat gatcgccgtc 1321 gatgaatcca gtttccggat catcggttac agtgaaaacg ccagagaaat gttagggatt 1381 atgcctcaat ctgttcctac tcttgagaaa cctgagattc tagctatggg aactgatgtg 1441 agatctttgt tcacttcttc gagctcgatt ctactcgagc gtgctttcgt tgctcgagag 1501 attaccttgt taaatccggt ttggatccat tccaagaata ctggtaaacc gttttacgcc 1561 attcttcata ggattgatgt tggtgttgtt attgatttag agccagctag aactgaagat 1621 cctgcgcttt ctattgctgg tgctgttcaa tcgcagaaac tcgcggttcg tgcgatttct 1681 cagttacagg ctcttcctgg tggagatatt aagcttttgt gtgacactgt cgtggaaagt 1741 gtgagggact tgactggtta tgatcgtgtt atggtttata agtttcatga agatgagcat 1801 ggagaagttg tagctgagag taaacgagac gatttagagc cttatattgg actgcattat 1861 cctgctactg atattcctca agcgtcaagg ttcttgttta agcagaaccg tgtccgaatg 1921 atagtagatt gcaatgccac acctgttctt gtggtccagg acgataggct aactcagtct 1981 atgtgcttgg ttggttctac tcttagggct cctcatggtt gtcactctca gtatatggct 2041 aacatgggat ctattgcgtc tttagcaatg gcggttataa tcaatggaaa tgaagatgat 2101 gggagcaatg tagctagtgg aagaagctcg atgaggcttt ggggtttggt tgtttgccat 2161 cacacttctt ctcgctgcat accgtttccg ctaaggtatg cttgtgagtt tttgatgcag 2221 gctttcggtt tacagttaaa catggaattg cagttagctt tgcaaatgtc agagaaacgc 2281 gttttgagaa cgcagacact gttatgtgat atgcttctgc gtgactcgcc tgctggaatt 2341 gttacacaga gtcccagtat catggactta gtgaaatgtg acggtgcagc atttctttac 2401 cacgggaagt attacccgtt gggtgttgct cctagtgaag ttcagataaa agatgttgtg 2461 gagtggttgc ttgcgaatca tgcggattca accggattaa gcactgatag tttaggcgat 2521 gcggggtatc ccggtgcagc tgcgttaggg gatgctgtgt gcggtatggc agttgcatat 2581 atcacaaaaa gagactttct tttttggttt cgatctcaca ctgcgaaaga aatcaaatgg 2641 ggaggcgcta agcatcatcc ggaggataaa gatgatgggc aacgaatgca tcctcgttcg 2701 tcctttcagg cttttcttga agttgttaag agccggagtc agccatggga aactgcggaa 2761 atggatgcga ttcactcgct ccagcttatt ctgagagact cttttaaaga atctggtggt 2821 tctgccggtg gctccggttc tggctccagc ggtggcagct ctggtgcgtc cggcacgggt 2881 actgcgggtg gcactggcag cggttccggt actggctctg gcaaccggaa atggtttccc 2941 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta 3001 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca 3061 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt 3121 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt 3181 cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg 3241 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc 3301 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc 3361 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt 3421 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat 3481 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt 3541 gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc 3601 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca 3661 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc 3721 cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattaggcg 3781 gtcgagcctc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat 3841 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat 3901 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa 3961 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc 4021 ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta 4081 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 4141 acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga 4201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 4261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 4321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 4381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 4441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 4621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 4681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 4741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 4801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 4921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 4981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 5041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 5101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 5161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 5221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 5281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 5341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 5401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 5461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 5521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 5581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 5641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 5701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 5761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 5821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 5881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 5941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 6001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 6061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 6121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 6181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 6241 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt 6301 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa 6361 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa 6421 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac 6481 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga 6541 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 6601 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc 6661 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc 6721 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 6781 agtcgaccgc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct 6841 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct 6901 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcgg //