LOCUS Exported 11287 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics . ACCESSION . VERSION . KEYWORDS p663-UBC-V5-BRSK1_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11287) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 11287) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11287 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 134..154 /label=T3 /note="T3 promoter, forward primer" promoter 136..154 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 182..408 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 409..589 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 636..761 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1254..1487 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1672..1716 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1983..2099 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2133..2153 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 2153..3364 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3201..3220 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind complement(3368..3388) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" LTR 3392..3660 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 3670..3711 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 3709..3733 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6074..6098) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6128..6716 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6181..6201) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6599..6610) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 6730..7229 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6765..6788) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7162..7181 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7250..7849 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7250..7269) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7746..7766 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7987..8220 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8292..8413 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8329..8348) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8383..8402 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8453..8588 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8515..8534 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8608..8627) /label=T7 /note="T7 promoter, forward primer" promoter complement(8609..8627) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8637..8654) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8637..8653) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8646..8668) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8795..9250 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8882..8901) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9092..9113 /label=F1ori-F /note="F1 origin, forward primer" promoter 9276..9380 /gene="bla" /label=AmpR promoter CDS 9381..10241 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9599..9618) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10412..11000 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10901..10920 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11154..11171 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg 2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca 2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc 2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac 2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag 2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat 2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc 2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg 2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct 2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa 2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg 2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg 2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa 2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg 2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc 3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc 3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc 3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg 3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag 3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa 3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt 3361 agacggccaa cttttgtata caaagtggcg aggggctcgc atctctcctt cacgcgcccg 3421 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg 3481 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg 3541 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct 3601 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc 3661 ggcaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac aactttgtac 3721 aaaaaagcag gcttaatgtc gtccggggcc aaggagggag gtgggggctc tcccgcctac 3781 cacctccccc acccccaccc ccacccaccc cagcacgccc aatatgtggg cccctatcgg 3841 ctggagaaga cgctgggcaa aggacagaca gggctggtta aactcggggt ccactgcatc 3901 acgggtcaga aggtcgccat caagatcgtg aaccgggaga agctgtcgga gtcggtgctg 3961 atgaaggtgg agcgggagat cgccatcctg aagctcatcg aacacccaca tgtcctcaag 4021 ctccacgacg tctacgagaa caagaaatat ttgtacctgg ttctggagca cgtctcgggg 4081 ggtgagctat tcgactacct ggtaaagaag gggagactga cgcccaagga ggcccgaaag 4141 ttcttccgcc agattgtgtc tgcgctggac ttctgccaca gctactccat ctgccacaga 4201 gacctaaagc ccgagaacct gcttttggat gagaaaaaca acatccgcat tgcagacttc 4261 ggcatggcgt ccctgcaggt gggggacagc ctcctggaga ccagctgcgg gtccccccat 4321 tatgcgtgtc cagaggtgat taagggggaa aaatatgatg gccgccgggc agacatgtgg 4381 agctgtggag tcatcctctt cgccctgctc gtgggggctc tgccctttga tgacgacaac 4441 ctccgccagc tgctggagaa ggtgaaacga ggcgtcttcc acatgcccca cttcattcct 4501 ccagattgcc agagcctcct gaggggaatg atcgaagtgg agcccgaaaa aaggctcagt 4561 ctggagcaaa ttcagaaaca tccttggtac ctaggcggga aacacgagcc agacccgtgc 4621 ctggagccag cccctggccg ccgggtagcc atgcggagcc tgccatccaa cggagagctg 4681 gaccccgacg tcctagagag catggcatca ctgggctgct tcagggaccg cgagaggctg 4741 catcgcgagc tgcgcagtga ggaggagaac caagaaaaga tgatatatta tctgcttttg 4801 gatcggaagg agcggtatcc cagctgtgag gaccaggacc tgcctccccg gaatgatgtt 4861 gacccccccc ggaagcgtgt ggattctccc atgctgagcc gtcacgggaa gcggcgacca 4921 gagcggaagt ccatggaagt cctgagcatc accgatgccg ggggtggtgg ctcccctgta 4981 cccacccgac gggccttgga gatggcccag cacagccaga gatcccgtag cgtcagtgga 5041 gcctccacgg gtctgtcctc cagccctcta agcagcccaa ggagtccggt cttttccttt 5101 tcaccggagc cgggggctgg agatgaggct cgaggcgggg gctccccgac ttccaaaacg 5161 cagacgctgc cttctcgggg ccccaggggt gggggcgccg gggagcagcc cccgcccccc 5221 agtgcccgct ccacacccct gcccggcccc ccaggctccc cgcgctcctc tggcgggacc 5281 cccttgcact cgcctctgca cacgccccgg gccagtccca ccgggacccc ggggacaaca 5341 ccacccccca gccccggcgg tggcgtcggg ggagccgcct ggaggagtcg tctcaactcc 5401 atccgcaaca gcttcctggg ctcccctcgc tttcaccggc gcaagatgca ggtccctacc 5461 gctgaggaga tgtccagctt gacgccagag tcctccccgg agctggcaaa acgctcctgg 5521 ttcgggaact tcatctcctt ggacaaagaa gaacaaatat tcctcgtgct aaaggacaaa 5581 cctctcagca gcatcaaagc agacatcgtc catgcctttc tgtcgatccc cagcctgagt 5641 cacagtgtgc tgtcacagac cagcttcagg gccgagtaca aggccagtgg cggcccctcc 5701 gtcttccaaa agcccgtccg cttccaggtg gacatcagct cctctgaggg tccagagccc 5761 tccccgcgac gggacggcag cggaggtggt ggcatctact ccgtcacctt cactctcatc 5821 tcgggtccca gccgtcggtt caagcgagtg gtggagacca tccaggcaca gctcctgagc 5881 actcatgacc agccctccgt gcaggccctg gcagacgaga agaacggggc ccagacccgg 5941 cctgctggtg ccccaccccg aagcctgcag cccccacccg gccgcccaga cccagagctg 6001 agcagctctc cccgccgagg cccccccaag gacaagaagc tcctggccac caacgggacc 6061 cctctgccct gaaacccagc tttcttgtac aaagttgtga tatccagcac agtggcggcc 6121 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 6181 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 6241 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 6301 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6361 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6421 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6481 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 6541 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6601 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6661 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 6721 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag 6781 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca 6841 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc 6901 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg 6961 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg 7021 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc 7081 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc 7141 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt 7201 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta 7261 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 7321 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 7381 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 7441 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 7501 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 7561 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 7621 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 7681 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 7741 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 7801 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca 7861 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac 7921 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7981 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg 8041 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 8101 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 8161 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 8221 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 8281 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 8341 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 8401 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 8461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 8521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 8581 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 8641 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 8701 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 8761 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 8821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 8881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 8941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 9001 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 9061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 9121 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 9181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 9241 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 9301 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 9361 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 9421 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 9481 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 9541 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 9601 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 9661 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 9721 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 9781 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 9841 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 9901 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 9961 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 10021 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 10081 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 10141 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 10201 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 10261 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 10321 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 10381 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 10441 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 10501 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 10561 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 10621 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 10681 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 10741 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 10801 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 10861 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 10921 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 10981 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 11041 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 11101 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 11161 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 11221 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 11281 acgcaat //