LOCUS Exported 11678 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics . ACCESSION . VERSION . KEYWORDS p663-UBC-TurboID-V5-BRSK2_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11678) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 11678) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11678 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 134..154 /label=T3 /note="T3 promoter, forward primer" promoter 136..154 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 182..408 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 409..589 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 636..761 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1254..1487 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1672..1716 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1983..2099 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2133..2153 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 2153..3364 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3201..3220 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind complement(3368..3388) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" regulatory 3389..3398 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3395..4357 /codon_start=1 /product="mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /label=TurboID /translation="MGKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" CDS 4364..4405 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 4406..4430 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6465..6489) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6519..7107 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6572..6592) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6990..7001) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 7121..7620 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7156..7179) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7553..7572 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7641..8240 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7641..7660) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8137..8157 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 8378..8611 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8683..8804 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8720..8739) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8774..8793 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8844..8979 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8906..8925 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8999..9018) /label=T7 /note="T7 promoter, forward primer" promoter complement(9000..9018) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9028..9045) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9028..9044) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9037..9059) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9186..9641 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9273..9292) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9483..9504 /label=F1ori-F /note="F1 origin, forward primer" promoter 9667..9771 /gene="bla" /label=AmpR promoter CDS 9772..10632 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9990..10009) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10803..11391 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11292..11311 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11545..11562 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg 2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca 2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc 2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac 2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag 2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat 2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc 2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg 2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct 2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa 2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg 2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg 2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa 2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg 2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc 3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc 3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc 3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg 3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag 3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa 3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt 3361 agacggccaa cttttgtata caaagtgggc caccatggga aaagacaata ctgtgcctct 3421 gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac 3481 cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt 3541 ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa 3601 cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga 3661 ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat 3721 tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg 3781 agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct 3841 gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt 3901 gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt 3961 ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt 4021 ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc 4081 agggattaac ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc 4141 cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga 4201 taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag 4261 ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg 4321 gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggct agcggcaagc ccatccccaa 4381 ccccctgctg ggcctggaca gcaccacaac tttgtacaaa aaagcagaag ggccgtcaag 4441 gcccaccatg acatcgacgg ggaaggacgg cggcgcgcag cacgcgcagt atgttgggcc 4501 ctaccggctg gagaagacgc tgggcaaggg gcagacaggt ctggtgaagc tgggggttca 4561 ctgcgtcacc tgccagaagg tggccatcaa gatcgtcaac cgtgagaagc tcagcgagtc 4621 ggtgctgatg aaggtggagc gggagatcgc gatcctgaag ctcattgagc acccccacgt 4681 cctaaagctg cacgacgttt atgaaaacaa aaaatatttg tacctggtgc tagaacacgt 4741 gtcaggtggt gagctcttcg actacctggt gaagaagggg aggctgacgc ctaaggaggc 4801 tcggaagttc ttccggcaga tcatctctgc gctggacttc tgccacagcc actccatatg 4861 ccacagggat ctgaaacctg aaaacctcct gctggacgag aagaacaaca tccgcatcgc 4921 agactttggc atggcgtccc tgcaggttgg cgacagcctg ttggagacca gctgtgggtc 4981 cccccactac gcctgccccg aggtgatccg gggggagaag tatgacggcc ggaaggcgga 5041 cgtgtggagc tgcggcgtca tcctgttcgc cttgctggtg ggggctctgc ccttcgacga 5101 tgacaacttg cgacagctgc tggagaaggt gaagcggggc gtgttccaca tgccgcactt 5161 tatcccgccc gactgccaga gtctgctacg gggcatgatc gaggtggacg ccgcacgccg 5221 cctcacgcta gagcacattc agaaacacat atggtatata gggggcaaga atgagcccga 5281 accagagcag cccattcctc gcaaggtgca gatccgctcg ctgcccagcc tggaggacat 5341 cgaccccgac gtgctggaca gcatgcactc actgggctgc ttccgagacc gcaacaagct 5401 gctgcaggac ctgctgtccg aggaggagaa ccaggagaag atgatttact tcctcctcct 5461 ggaccggaaa gaaaggtacc cgagccagga ggatgaggac ctgccccccc ggaacgagat 5521 agaccctccc cggaagcgtg tggactcccc gatgctgaac cggcacggca agcggcggcc 5581 agaacgcaaa tccatggagg tgctcagcgt gacggacggc ggctccccgg tgcctgcgcg 5641 gcgggccatt gagatggccc agcacggcca gaggtctcgg tccatcagcg gtgcctcctc 5701 aggcctttcc accagcccac tcagcagccc ccgggtgacc cctcacccct caccaagggg 5761 cagtcccctc cccaccccca aggggacacc tgtccacacg ccaaaggaga gcccggctgg 5821 cacgcccaac cccacgcccc cgtccagccc cagcgtcgga ggggtgccct ggagggcgcg 5881 gctcaactcc atcaagaaca gctttctggg ctcaccccgc ttccaccgcc ggaaactgca 5941 agttccgacg ccggaggaga tgtccaacct gacaccagag tcgtccccag agctggcgaa 6001 gaagtcctgg tttgggaact tcatcagcct ggagaaggag gagcagatct tcgtggtcat 6061 caaagacaaa cctctgagct ccatcaaggc tgacatcgtg cacgccttcc tgtcgattcc 6121 cagtctcagc cacagcgtca tctcccaaac gagcttccgg gccgagtaca aggccacggg 6181 ggggccagcc gtgttccaga agccggtcaa gttccaggtt gatatcacct acacggaggg 6241 tggggaggcg cagaaggaga acggcatcta ctccgtcacc ttcaccctgc tctcaggccc 6301 cagccgtcgc ttcaagaggg tggtggagac catccaggcc cagctgctga gcacacacga 6361 cccgcctgcg gcccagcact tgtcagacac cactaactgt atggaaatga tgacggggcg 6421 gctttccaaa tgtggaatta tcccgaaaag ttgaggcctc atgggcccag ctttcttgta 6481 caaagttgtg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa 6541 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 6601 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 6661 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 6721 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 6781 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 6841 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 6901 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 6961 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 7021 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 7081 ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc 7141 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt 7201 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt 7261 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg 7321 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag 7381 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag 7441 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc 7501 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc 7561 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg 7621 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 7681 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 7741 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 7801 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 7861 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 7921 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 7981 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 8041 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 8101 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 8161 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 8221 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg 8281 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct 8341 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa 8401 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 8461 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 8521 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 8581 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 8641 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 8701 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 8761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 8821 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 8881 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 8941 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 9001 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 9061 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 9121 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 9181 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 9241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 9301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 9361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 9421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 9481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 9541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 9601 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 9661 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 9721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 9781 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 9841 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 9901 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 9961 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 10021 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 10081 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 10141 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 10201 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 10261 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 10321 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 10381 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 10441 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 10501 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 10561 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 10621 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 10681 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 10741 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 10801 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 10861 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 10921 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 10981 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 11041 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 11101 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 11161 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 11221 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 11281 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 11341 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 11401 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 11461 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 11521 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 11581 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 11641 tttcccgact ggaaagcggg cagtgagcgc aacgcaat //