LOCUS Exported 10981 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway destination clone of BRSK2 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics . ACCESSION . VERSION . KEYWORDS p663-UBC-V5-BRSK2_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10981) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 10981) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10981 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 134..154 /label=T3 /note="T3 promoter, forward primer" promoter 136..154 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 182..408 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 409..589 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 636..761 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1254..1487 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1672..1716 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1983..2099 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2133..2153 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 2153..3364 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3201..3220 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind complement(3368..3388) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" LTR 3392..3660 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 3670..3711 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 3709..3733 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5768..5792) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 5822..6410 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5875..5895) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6293..6304) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 6424..6923 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6459..6482) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6856..6875 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6944..7543 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6944..6963) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7440..7460 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7681..7914 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7986..8107 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8023..8042) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8077..8096 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8147..8282 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8209..8228 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8302..8321) /label=T7 /note="T7 promoter, forward primer" promoter complement(8303..8321) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8331..8348) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8331..8347) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8340..8362) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8489..8944 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8576..8595) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8786..8807 /label=F1ori-F /note="F1 origin, forward primer" promoter 8970..9074 /gene="bla" /label=AmpR promoter CDS 9075..9935 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9293..9312) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10106..10694 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10595..10614 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10848..10865 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg 2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca 2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc 2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac 2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag 2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat 2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc 2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg 2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct 2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa 2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg 2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg 2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa 2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg 2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc 3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc 3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc 3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg 3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag 3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa 3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt 3361 agacggccaa cttttgtata caaagtggcg aggggctcgc atctctcctt cacgcgcccg 3421 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg 3481 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg 3541 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct 3601 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc 3661 ggcaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac aactttgtac 3721 aaaaaagcag aagggccgtc aaggcccacc atgacatcga cggggaagga cggcggcgcg 3781 cagcacgcgc agtatgttgg gccctaccgg ctggagaaga cgctgggcaa ggggcagaca 3841 ggtctggtga agctgggggt tcactgcgtc acctgccaga aggtggccat caagatcgtc 3901 aaccgtgaga agctcagcga gtcggtgctg atgaaggtgg agcgggagat cgcgatcctg 3961 aagctcattg agcaccccca cgtcctaaag ctgcacgacg tttatgaaaa caaaaaatat 4021 ttgtacctgg tgctagaaca cgtgtcaggt ggtgagctct tcgactacct ggtgaagaag 4081 gggaggctga cgcctaagga ggctcggaag ttcttccggc agatcatctc tgcgctggac 4141 ttctgccaca gccactccat atgccacagg gatctgaaac ctgaaaacct cctgctggac 4201 gagaagaaca acatccgcat cgcagacttt ggcatggcgt ccctgcaggt tggcgacagc 4261 ctgttggaga ccagctgtgg gtccccccac tacgcctgcc ccgaggtgat ccggggggag 4321 aagtatgacg gccggaaggc ggacgtgtgg agctgcggcg tcatcctgtt cgccttgctg 4381 gtgggggctc tgcccttcga cgatgacaac ttgcgacagc tgctggagaa ggtgaagcgg 4441 ggcgtgttcc acatgccgca ctttatcccg cccgactgcc agagtctgct acggggcatg 4501 atcgaggtgg acgccgcacg ccgcctcacg ctagagcaca ttcagaaaca catatggtat 4561 atagggggca agaatgagcc cgaaccagag cagcccattc ctcgcaaggt gcagatccgc 4621 tcgctgccca gcctggagga catcgacccc gacgtgctgg acagcatgca ctcactgggc 4681 tgcttccgag accgcaacaa gctgctgcag gacctgctgt ccgaggagga gaaccaggag 4741 aagatgattt acttcctcct cctggaccgg aaagaaaggt acccgagcca ggaggatgag 4801 gacctgcccc cccggaacga gatagaccct ccccggaagc gtgtggactc cccgatgctg 4861 aaccggcacg gcaagcggcg gccagaacgc aaatccatgg aggtgctcag cgtgacggac 4921 ggcggctccc cggtgcctgc gcggcgggcc attgagatgg cccagcacgg ccagaggtct 4981 cggtccatca gcggtgcctc ctcaggcctt tccaccagcc cactcagcag cccccgggtg 5041 acccctcacc cctcaccaag gggcagtccc ctccccaccc ccaaggggac acctgtccac 5101 acgccaaagg agagcccggc tggcacgccc aaccccacgc ccccgtccag ccccagcgtc 5161 ggaggggtgc cctggagggc gcggctcaac tccatcaaga acagctttct gggctcaccc 5221 cgcttccacc gccggaaact gcaagttccg acgccggagg agatgtccaa cctgacacca 5281 gagtcgtccc cagagctggc gaagaagtcc tggtttggga acttcatcag cctggagaag 5341 gaggagcaga tcttcgtggt catcaaagac aaacctctga gctccatcaa ggctgacatc 5401 gtgcacgcct tcctgtcgat tcccagtctc agccacagcg tcatctccca aacgagcttc 5461 cgggccgagt acaaggccac gggggggcca gccgtgttcc agaagccggt caagttccag 5521 gttgatatca cctacacgga gggtggggag gcgcagaagg agaacggcat ctactccgtc 5581 accttcaccc tgctctcagg ccccagccgt cgcttcaaga gggtggtgga gaccatccag 5641 gcccagctgc tgagcacaca cgacccgcct gcggcccagc acttgtcaga caccactaac 5701 tgtatggaaa tgatgacggg gcggctttcc aaatgtggaa ttatcccgaa aagttgaggc 5761 ctcatgggcc cagctttctt gtacaaagtt gtgatatcca gcacagtggc ggccgctcga 5821 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5881 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5941 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6001 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6061 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6121 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6181 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6241 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6301 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6361 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct 6421 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc 6481 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta 6541 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag 6601 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag 6661 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc 6721 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa 6781 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt 6841 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct 6901 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc 6961 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 7021 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 7081 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 7141 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 7201 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 7261 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 7321 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 7381 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 7441 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 7501 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc 7561 gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa 7621 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 7681 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 7921 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 7981 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8041 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8101 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 8161 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 8221 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 8281 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 8341 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 8401 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 8461 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 8521 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 8581 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 8641 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 8701 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 8761 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 8821 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 8881 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 8941 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 9001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 9061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 9121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 9181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 9241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 9301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 9361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 9421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 9481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 9541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 9601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 9661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 9721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 9781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 9841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 9901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 9961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 10021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 10081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 10141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 10201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 10261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 10321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 10381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 10441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 10501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 10561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 10621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 10681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 10741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 10801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 10861 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 10921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 10981 t //