LOCUS Exported 7003 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS p5077 pcDNA4c hBrd4 (1-594) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7003) AUTHORS You J, Croyle JL, Nishimura A, Ozato K, Howley PM TITLE Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. JOURNAL Cell. 2004 Apr 30. 117(3):349-60. PUBMED 15109495 REFERENCE 2 (bases 1 to 7003) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7003 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 917..1079 /label=QBI SP163 /note="strong translational enhancer derived from the 5' UTR of the mouse VEGF gene" CDS 1092..1109 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 1113..1145 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 1149..1172 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" primer_bind complement(3011..3028) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3017..3241 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3287..3715 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3374..3393) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3584..3605 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3724..3744) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3729..4058 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3909..4044 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3971..3990 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 4106..4153 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 4172..4546 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 4676..4797 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4713..4732) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4767..4786 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4846..4862) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4846..4862) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4859..4881) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4870..4886 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4894..4924) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4939..4960 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5077..5094) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5248..5836) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5328..5347) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6007..6867) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6630..6649 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6868..6972) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttagcg cagaggcttg gggcagccga gcggcagcca ggccccggcc 961 cgggcctcgg ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct 1021 cgcagtccga gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca 1081 tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa 1141 tgggtcggga tctgtacgac gatgacgata aggtaccagg atccatgtct gcggagagcg 1201 gccctgggac gagattgaga aatctgccag taatggggga tggactagaa acttcccaaa 1261 tgtctacaac acaggcccag gcccaacccc agccagccaa cgcagccagc accaaccccc 1321 cgcccccaga gacctccaac cctaacaagc ccaagaggca gaccaaccaa ctgcaatacc 1381 tgctcagagt ggtgctcaag acactatgga aacaccagtt tgcatggcct ttccagcagc 1441 ctgtggatgc cgtcaagctg aacctccctg attactataa gatcattaaa acgcctatgg 1501 atatgggaac aataaagaag cgcttggaaa acaactatta ctggaatgct caggaatgta 1561 tccaggactt caacactatg tttacaaatt gttacatcta caacaagcct ggagatgaca 1621 tagtcttaat ggcagaagct ctggaaaagc tcttcttgca aaaaataaat gagctaccca 1681 cagaagaaac cgagatcatg atagtccagg caaaaggaag aggacgtggg aggaaagaaa 1741 cagggacagc aaaacctggc gtttccacgg taccaaacac aactcaagca tcgactcctc 1801 cgcagaccca gacccctcag ccgaatcctc ctcctgtgca ggccacgcct caccccttcc 1861 ctgccgtcac cccggacctc atcgtccaga cccctgtcat gacagtggtg cctccccagc 1921 cactgcagac gcccccgcca gtgccccccc agccacaacc cccacccgct ccagctcccc 1981 agcccgtaca gagccaccca cccatcatcg cggccacccc acagcctgtg aagacaaaga 2041 agggagtgaa gaggaaagca gacaccacca cccccaccac cattgacccc attcacgagc 2101 caccctcgct gcccccggag cccaagacca ccaagctggg ccagcggcgg gagagcagcc 2161 ggcctgtgaa acctccaaag aaggacgtgc ccgactctca gcagcaccca gcaccagaga 2221 agagcagcaa ggtctcggag cagctcaagt gctgcagcgg catcctcaag gagatgtttg 2281 ccaagaagca cgccgcctac gcctggccct tctacaagcc tgtggacgtg gaggcactgg 2341 gcctacacga ctactgtgac atcatcaagc accccatgga catgagcaca atcaagtcta 2401 aactggaggc ccgtgagtac cgtgatgctc aggagtttgg tgctgacgtc cgattgatgt 2461 tctccaactg ctataagtac aaccctcctg accatgaggt ggtggccatg gcccgcaagc 2521 tccaggatgt gttcgaaatg cgctttgcca agatgccgga cgagcctgag gagccagtgg 2581 tggccgtgtc ctccccggca gtgccccctc ccaccaaggt tgtggccccg ccctcatcca 2641 gcgacagcag cagcgatagc tcctcggaca gtgacagttc gactgatgac tctgaggagg 2701 agcgagccca gcggctggct gagctccagg agcagctcaa agccgtgcac gagcagcttg 2761 cagccctctc tcagccccag cagaacaaac caaagaaaaa ggagaaagac aagaaggaaa 2821 agaaaaaaga aaagcacaaa aggaaagagg aagtggaaga gaataaaaaa agcaaagcca 2881 aggaacctcc tcctaaaaag acgaagaaaa ataatagcag caacagcaat gtgagcaaga 2941 aggagccagc gcccatgaag agcaaggcgg ccgctcgagt ctagagggcc cgtttaaacc 3001 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 3061 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 3121 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 3181 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 3241 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 3301 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3361 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3421 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 3481 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 3541 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 3601 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 3661 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 3721 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 3781 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 3841 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 3901 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 3961 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 4021 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 4081 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat 4141 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt 4201 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc 4261 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat 4321 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg 4381 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc 4441 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga 4501 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga 4561 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 4621 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 4681 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 4741 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 4801 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 4861 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 4921 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 4981 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 5041 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5101 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5161 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5221 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 5281 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 5341 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 5401 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 5461 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 5521 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 5581 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 5641 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 5701 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 5761 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 5821 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5881 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 5941 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6001 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6061 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6121 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6181 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 6241 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 6301 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 6361 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 6421 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 6481 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 6541 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 6601 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 6661 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 6721 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 6781 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 6841 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6901 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 6961 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc //