LOCUS       Exported                7113 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  ZC3H15 3'UTR (NM_018471.2) inserted into the 3'UTR of Renilla 
            luciferase in the psiCHECK3 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    psiCHECK3-ZC3H15
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Fischer , J.W., Busa, V.F., Shao, S. Leung, A. K.L.
  TITLE     Structure-mediated RNA decay by UPF1 and G3BP1
  JOURNAL   Molecular Cell, Feb 3, 2020
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        71..428
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(94..114)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      279..414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     341..360
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          498..630
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        675..693
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      697..706
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             703..1638
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2312..2360
                     /note="synthetic polyadenylation signal"
     promoter        2369..3120
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          3190..3322
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        3367..3385
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3394..3403
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3400..5052
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             4359..4367
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5077..5198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5088..5107)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5142..5161
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5331..5435
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5436..6296
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5654..5673)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6467..7055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6956..6975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggctcgaca gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
       61 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
      121 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      181 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      241 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
      361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      421 tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaagctgc agaagttggt
      481 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
      541 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      601 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt
      661 aaggctagag tacttaatac gactcactat aggctagcca ccatggcttc caaggtgtac
      721 gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa
      781 atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc
      841 gtgatttttc tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac
      901 atcgagcccg tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag
      961 agcgggaatg gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag
     1021 ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc
     1081 tttcactact cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc
     1141 gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc
     1201 aagagcgaag agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc
     1261 ccaagcaaga tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc
     1321 aaggagaagg gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt
     1381 aagggaggca agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc
     1441 agcgacgatc tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt
     1501 gtcgagggag ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc
     1561 agccaggagg acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg
     1621 ctgaagaacg agcagtaatt ctaggcgatc gctcgagcac caaacacatc gctgaaaaaa
     1681 ttaagtcagc tcagcacgag ttgaaattga ctacattaat ttctttccac ctagaatcaa
     1741 caggatgttt atttcctatg ctgattctgg aggagttaac ctcctgcaaa aaaggcatct
     1801 tgtccctaca tcttctcttc tgactttggc tacatctcat agtaagttca gagtagttca
     1861 tgataaattg aaaatataat ggtcattgca gaaaatgatt gatgttgtaa ctgtccaccc
     1921 aagtaagaag tgtatctgcc tttccatctt ttggttttca tttgggcatg tgctattacc
     1981 agaaacaaca aacttatatt taaaataccc ttcatttgac acagttttta atgagtgatt
     2041 taatttcctc tgtatttgta tgtttagaag actgcctaaa acatgagcac tgtacttcat
     2101 aaaggaaact gcgtatgcag attcagtatt gtgtatcttt ggacaattag atggacattt
     2161 aaaatggaac ttctttatct gacaggatca gctacaatgc cctgtgttaa attgtttaaa
     2221 agtttccctt ttcttttttg ccaataaagt tgtaaataaa gaccatcata cattaaaatc
     2281 caagtttaaa cctagagcgg ccgctggccg caataaaata tctttatttt cattacatct
     2341 gtgtgttggt tttttgtgtg aggatctaaa tgagtcttcg gacctcgcgg gggccgctta
     2401 agcggtggtt agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac actctcattc
     2461 ggaggcggct cggggtttgg tcttggtggc cacgggcacg cagaagagcg ccgcgatcct
     2521 cttaagcacc cccccgccct ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg
     2581 ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga
     2641 cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct ccatggggac
     2701 cgtatacgtg gacaggctct ggagcatccg cacgactgcg gtgatattac cggagacctt
     2761 ctgcgggacg agccgggtca cgcggctgac gcggagcgtc cgttgggcga caaacaccag
     2821 gacggggcac aggtacacta tcttgtcacc cggaggcgcg agggactgca ggagcttcag
     2881 ggagtggcgc agctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc
     2941 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt
     3001 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc
     3061 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa
     3121 aagcttgatt cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc
     3181 actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc
     3241 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc
     3301 cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag
     3361 agtacttaat acgactcact ataggctagc aaagccacca tggccgatgc taagaacatt
     3421 aagaagggcc ctgctccctt ctaccctctg gaggatggca ccgctggcga gcagctgcac
     3481 aaggccatga agaggtatgc cctggtgcct ggcaccattg ccttcaccga tgcccacatt
     3541 gaggtggaca tcacctatgc cgagtacttc gagatgtctg tgcgcctggc cgaggccatg
     3601 aagaggtacg gcctgaacac caaccaccgc atcgtggtgt gctctgagaa ctctctgcag
     3661 ttcttcatgc cagtgctggg cgccctgttc atcggagtgg ccgtggcccc tgctaacgac
     3721 atttacaacg agcgcgagct gctgaacagc atgggcattt ctcagcctac cgtggtgttc
     3781 gtgtctaaga agggcctgca gaagatcctg aacgtgcaga agaagctgcc tatcatccag
     3841 aagatcatca tcatggactc taagaccgac taccagggct tccagagcat gtacacattc
     3901 gtgacatctc atctgcctcc tggcttcaac gagtacgact tcgtgccaga gtctttcgac
     3961 agggacaaaa ccattgccct gatcatgaac agctctgggt ctaccggcct gcctaagggc
     4021 gtggccctgc ctcatcgcac cgcctgtgtg cgcttctctc acgcccgcga ccctattttc
     4081 ggcaaccaga tcatccccga caccgctatt ctgagcgtgg tgccattcca ccacggcttc
     4141 ggcatgttca ccaccctggg ctacctgatt tgcggctttc gggtggtgct gatgtaccgc
     4201 ttcgaggagg agctgttcct gcgcagcctg caagactaca aaattcagtc tgccctgctg
     4261 gtgccaaccc tgttcagctt cttcgctaag agcaccctga tcgacaagta cgacctgtct
     4321 aacctgcacg agattgcctc tggcggcgcc ccactgtcta aggaggtggg cgaagccgtg
     4381 gccaagcgct ttcatctgcc aggcatccgc cagggctacg gcctgaccga gacaaccagc
     4441 gccattctga ttaccccaga gggcgacgac aagcctggcg ccgtgggcaa ggtggtgcca
     4501 ttcttcgagg ccaaggtggt ggacctggac accggcaaga ccctgggagt gaaccagcgc
     4561 ggcgagctgt gtgtgcgcgg ccctatgatt atgtccggct acgtgaataa ccctgaggcc
     4621 acaaacgccc tgatcgacaa ggacggctgg ctgcactctg gcgacattgc ctactgggac
     4681 gaggacgagc acttcttcat cgtggaccgc ctgaagtctc tgatcaagta caagggctac
     4741 caggtggccc cagccgagct ggagtctatc ctgctgcagc accctaacat tttcgacgcc
     4801 ggagtggccg gcctgcccga cgacgatgcc ggcgagctgc ctgccgccgt cgtcgtgctg
     4861 gaacacggca agaccatgac cgagaaggag atcgtggact atgtggccag ccaggtgaca
     4921 accgccaaga agctgcgcgg cggagtggtg ttcgtggacg aggtgcccaa gggcctgacc
     4981 ggcaagctgg acgcccgcaa gatccgcgag atcctgatca aggctaagaa aggcggcaag
     5041 atcgccgtgt aatgcggccg cttcgagcag acatgataag atacattgat gagtttggac
     5101 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5161 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5221 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5281 aatgtggtaa aatcgataag gatccaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5341 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5401 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5461 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5521 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5581 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5641 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5701 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5761 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5821 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5881 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5941 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6001 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6061 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6301 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6361 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6421 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6601 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6841 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7081 ggttcctggc cttttgctgg ccttttgctc aca
//
