LOCUS Exported 11147 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.. ACCESSION . VERSION . KEYWORDS pJFRC-nSyb-IVS-PhiC31 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11147) AUTHORS Isaacman-Beck J, Paik K, Wienecke C, Yang H, Fisher Y, Wang I, Ishida I, Maimon G, Wilson R, Clandinin T TITLE SPARC: a method to genetically manipulate precise proportions of cells. JOURNAL bioRxiv REFERENCE 2 (bases 1 to 11147) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11147 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..1815 /codon_start=1 /product="integrase from phage phi-C31" /label=phage phi-C31 integrase /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI EKRASITWAKPPTDDDEDDAQDGTEDVAA" CDS 1816..1836 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 1926..1991 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2121..2141 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 2413..2547 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2450..2469) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2504..2523 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(2589..2607) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2674..2778 /gene="bla" /label=AmpR promoter CDS 2779..3639 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2997..3016) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3810..4398 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4299..4318 /label=pBR322ori-F /note="pBR322 origin, forward primer" protein_bind 4485..4554 /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" primer_bind complement(4779..4797) /label=pCasper-F /note="Drosophila mini-white gene, reverse primer" CDS join(5249..5320,5722..5995,6070..6724,6786..7101,7305..7436, 7507..8121) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /label=mini-white /note="This is a modified version of the white gene lacking part of the first intron." /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" intron 11067..11133 /label=Mhc intron /note="modified Drosophila myosin heavy chain intron 16 (Pfeiffer et al., 2010)" regulatory 11142..4 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 atggacacgt acgcgggtgc ttacgaccgt cagtcgcgcg agcgcgaaaa ttcgagcgca 61 gcaagcccag cgacacagcg tagcgccaac gaagacaagg cggccgacct tcagcgcgaa 121 gtcgagcgcg acgggggccg gttcaggttc gtcgggcatt tcagcgaagc gccgggcacg 181 tcggcgttcg ggacggcgga gcgcccggag ttcgaacgca tcctgaacga atgccgcgcc 241 gggcggctca acatgatcat tgtctatgac gtgtcgcgct tctcgcgcct gaaggtcatg 301 gacgcgattc cgattgtctc ggaattgctc gccctgggcg tgacgattgt ttccactcag 361 gaaggcgtct tccggcaggg aaacgtcatg gacctgattc acctgattat gcggctcgac 421 gcgtcgcaca aagaatcttc gctgaagtcg gcgaagattc tcgacacgaa gaaccttcag 481 cgcgaattgg gcgggtacgt cggcgggaag gcgccttacg gcttcgagct tgtttcggag 541 acgaaggaga tcacgcgcaa cggccgaatg gtcaatgtcg tcatcaacaa gcttgcgcac 601 tcgaccactc cccttaccgg acccttcgag ttcgagcccg acgtaatccg gtggtggtgg 661 cgtgagatca agacgcacaa acaccttccc ttcaagccgg gcagtcaagc cgccattcac 721 ccgggcagca tcacggggct ttgtaagcgc atggacgctg acgccgtgcc gacccggggc 781 gagacgattg ggaagaagac cgcttcaagc gcctgggacc cggcaaccgt tatgcgaatc 841 cttcgggacc cgcgtattgc gggcttcgcc gctgaggtga tctacaagaa gaagccggac 901 ggcacgccga ccacgaagat tgagggttac cgcattcagc gcgacccgat cacgctccgg 961 ccggtcgagc ttgattgcgg accgatcatc gagcccgctg agtggtatga gcttcaggcg 1021 tggttggacg gcagggggcg cggcaagggg ctttcccggg ggcaagccat tctgtccgcc 1081 atggacaagc tgtactgcga gtgtggcgcc gtcatgactt cgaagcgcgg ggaagaatcg 1141 atcaaggact cttaccgctg ccgtcgccgg aaggtggtcg acccgtccgc acctgggcag 1201 cacgaaggca cgtgcaacgt cagcatggcg gcactcgaca agttcgttgc ggaacgcatc 1261 ttcaacaaga tcaggcacgc cgaaggcgac gaagagacgt tggcgcttct gtgggaagcc 1321 gcccgacgct tcggcaagct cactgaggcg cctgagaaga gcggcgaacg ggcgaacctt 1381 gttgcggagc gcgccgacgc cctgaacgcc cttgaagagc tgtacgaaga ccgcgcggca 1441 ggcgcgtacg acggacccgt tggcaggaag cacttccgga agcaacaggc agcgctgacg 1501 ctccggcagc aaggggcgga agagcggctt gccgaacttg aagccgccga agccccgaag 1561 cttccccttg accaatggtt ccccgaagac gccgacgctg acccgaccgg ccctaagtcg 1621 tggtgggggc gcgcgtcagt agacgacaag cgcgtgttcg tcgggctctt cgtagacaag 1681 atcgttgtca cgaagtcgac tacgggcagg gggcagggaa cgcccatcga gaagcgcgct 1741 tcgatcacgt gggcgaagcc gccgaccgac gacgacgaag acgacgccca ggacggcacg 1801 gaagacgtag cggcgcctaa gaagaagagg aaggtttagc tctctagagg atctttgtga 1861 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct 1921 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt 1981 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta 2041 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg 2101 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc 2161 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct 2221 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat 2281 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt 2341 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 2401 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 2461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 2521 atcaatgtat cttatcatgt ctggatcgat ctggccggcc gtttaaacga attcttgaag 2581 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 2641 ttagactcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 2701 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2761 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 2821 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 2881 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 2941 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 3001 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 3061 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 3121 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 3181 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 3241 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 3301 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 3361 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 3421 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 3481 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 3541 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 3601 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 3661 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3721 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3781 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3901 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3961 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 4021 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 4081 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 4141 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 4201 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 4261 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 4321 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4381 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4441 tggccttttg ctcacatgtt accgtcgacg atgtaggtca cggtctcgaa gccgcggtgc 4501 gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc 4561 atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg 4621 aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg 4681 gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt tctcgacggt cacggcgggc 4741 atgtcgacaa gccgaacata tgggcgcgcc tagtatgtat gtaagttaat aaaacccatt 4801 tttgcggaaa gtagataaaa aaaacatttt ttttttttac tgcactggat atcattgaac 4861 ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgatgtacc aggttctttc 4921 gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc ctccttctct 4981 gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt 5041 tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg gtttcgtgac 5101 gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa aactcctctc 5161 gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt gggtgggtaa 5221 gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc tattaattcg 5281 cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta catgtgcata 5341 catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg tctgcggcgt 5401 gagaactgcg acccacaaaa atcccaaacc gcaattgcac aaacaaatag tgacacgaaa 5461 cagattattc tggtagctgt tctcgctata taagacaatt tttgagatca tatcatgatc 5521 aagacatcta aaggcattca ttttcgacta tattcttttt tacaaaaaat ataacaacca 5581 gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac ctacttcgta 5641 ggatacttcg gggtactttt tgttcggggt tagatgagca taacgcttgt agttgatatt 5701 tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag 5761 ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc ggaggactcc 5821 ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc 5881 tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga 5941 ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag 6001 tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt 6061 gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg 6121 gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag 6181 tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg 6241 ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac 6301 acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg 6361 cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg 6421 gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct 6481 ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact 6541 cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg 6601 tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc 6661 tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt 6721 cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc tcaactccta 6781 tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg 6841 ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac 6901 aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatctg 6961 gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag 7021 ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa 7081 gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac 7141 aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca cacccttctt 7201 agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa atttcattta 7261 actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga 7321 ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat atcaacggag 7381 ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa 7441 gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg aatcgattcg 7501 atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat 7561 cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg 7621 ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac 7681 ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat 7741 ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc 7801 ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc 7861 aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa 7921 tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt 7981 tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac 8041 gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt 8101 cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt 8161 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat 8221 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc 8281 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac 8341 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt 8401 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt 8461 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg 8521 actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc 8581 tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta 8641 tatatttccc tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt 8701 tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt 8761 tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata 8821 tcaaaaataa caactataat aataagaata catttaattt agaaaatgct tggatttcac 8881 tggaactagg gcgcgcctcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag 8941 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt 9001 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag 9061 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg 9121 ccagggccgc aagcttggcc ggccagatct gacctcaaag atggaagcgg agcagaagga 9181 gcgatttcag acagacgccc ccaagttttg ggattccttc tacggcattc acgacaaccg 9241 cttcttcaag gatcgccact ggctgttcac ggagttcccc gaactagctc ctctggcggc 9301 ggattcggcg gtactgcaac cacgcagcat tttcgaactg ggctgtggag taggaaacac 9361 aattttaccc cttctacagt acagctctga accgcagctc aaggttttcg gatgtgactt 9421 ctccgcccgg gccattgaaa tcctgcgaag ccaacggcag ttcgacgaga agcgctgcga 9481 ggtgtttgtc atggatgcca cgctggatca ttggcaggtg cccttcgagg aaaactcaca 9541 ggacattatt gtgatgattt ttgtgctgtc cgcaattgag ccaaagaaaa tgcagcgggt 9601 attggacaat tgctatcgct acctgcgtcc cggtggcttg ctcctcttcc gcgactacgg 9661 acgatacgac ctggcacagc tgcgcttcaa gagcggcaag tgtatggagg acaacttcta 9721 cgtgcgaggc gacggcacca tggtgtactt ctttacagag gaagaactgc ggggtatgat 9781 gactcaagcg gggctgcagg aggagcagct cattgtggac cgaagactgc aagttaatcg 9841 ctgtcgcggc ttgaagatgt accgcgtgtg gattcagaca aagttcagga aaccgcttta 9901 aatcaattta gacgaatagt tctgtgcatt ttaaaccttg ttcaaatcaa ttgatataat 9961 tatttacacg atatatttaa aaatacaaag ttaaaacttc aaactgtaat attactagaa 10021 ctttgagcat ttctttttct tgatggaaag cccggcttgt tttcaaattt cccacccctt 10081 ggccatcggc acttggaaat cgctaaggaa aagcttctcg ctggaacacc attcgctgct 10141 ggccatccag gcagcttttt ccccaaatac acataacagt taggaaagct gcaagttttc 10201 gcagttggcc aaaaacgtcg taagtgaaaa ttttcctggt gctgcgtaaa aaaattcgta 10261 aaatattagt taagcctggt ttttgtaaac tcaagttgcc cgagcaaaag taaagtgatt 10321 ctgatcaaaa gtgctaaaac tgattgatta ggaaaacgaa gatcaaacga aatgtcaacc 10381 aaagtcagcg aaaacccttg tcgtgcagaa tttcattgat tttttcttct gagcccacgt 10441 tggccagttg ggcacttgaa attattttta aaacgttttg cattttgaag acatcttagg 10501 tgcccctggg ggtatgcaac ggaaatgagg aaggacatgc ctcatggctg ggcgtgttgc 10561 tgccacccaa atagtcctct tacgtcccaa ttagaatccc atagctgccc atttcaagca 10621 ttaactgatg tactaaaaat agttgcaact attttcgcac tctattcagc agcagttcca 10681 gcgctgagcc agaaaattcc tgcgaaaggc tgtcgtggca agtgttaccc gcacacatac 10741 acatcatcca gatccagcca atccccccgt ggagtggagt gaattttcat tgacgacggg 10801 gcgaaaaagc tctggcggaa gagcgacaga gagagagaga aacagagaga gagagagaga 10861 agcggcactg agagcgcaac cctctagaac aaaaacaacg aaacagagtt cagctgcacc 10921 accacccact acctacacca ccaacgccac caccgcccac tgaccatccc acatcccgac 10981 ctgcgccagt ggaagacagt gaaagaggcc aagtgcatcg tgcatagagt accttcatcc 11041 taatcatcga cagccagatc taaaaggtag gttcaaccac tgatgcctag gcacaccgaa 11101 acgactaacc ctaattctta tcctttactt caggcggccg cgccacc //