LOCUS       Exported               11168 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal V5 
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    p667-UBC-TLK2-V5_IDG-K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11168)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        33..63
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    71..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     76..98
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     95..111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     95..111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     130..150
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        132..150
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        178..404
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1979..2095
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2127..2147
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2147..3358
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3195..3214
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3359..3383
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3961..3972)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5697..5721)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5720..5761
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5820..5839)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(5956..5976)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6005..6593
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6058..6078)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6476..6487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6607..7106
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6642..6665)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7039..7058
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7127..7726
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7127..7146)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7623..7643
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7864..8097
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8169..8290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8206..8225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8260..8279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8330..8465
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8392..8411
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8485..8504)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8486..8504)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8514..8531)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8514..8530)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8523..8545)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8672..9127
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8759..8778)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8969..8990
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9153..9257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9258..10118
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9476..9495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10289..10877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10778..10797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11031..11048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11165..18
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
       61 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      121 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
      181 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
      241 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
      301 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
      361 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     1981 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2041 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcgt
     2101 ccagtgtggt ggaattctgc agatatcaac tttgtataga aaagttggcc tccgcgccgg
     2161 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
     2221 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
     2281 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
     2341 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
     2401 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
     2461 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
     2521 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
     2581 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
     2641 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
     2701 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
     2761 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     2821 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     2881 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     2941 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     3001 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     3061 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     3121 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     3181 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     3241 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     3301 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacgc
     3361 aactttgtac aaaaaagttg gcatggaaga attgcatagc ctggacccac gacggcagga
     3421 attattggag gccaggttta ctggagtagg tgttagtaag ggaccactta atagtgagtc
     3481 ttccaaccag agcttgtgca gcgtcggatc cttgagtgat aaagaagtag agactcccga
     3541 gaaaaagcag aatgaccagc gaaatcggaa aagaaaagct gaaccatatg aaactagcca
     3601 agggaaaggc actcctaggg gacataaaat tagtgattac tttgagtttg ctgggggaag
     3661 cgcgccagga accagccctg gcagaagtgt tccaccagtt gcacgatcct caccgcaaca
     3721 ttccttatcc aatcccttac cgcgacgagt agaacagccc ctctatggtt tagatggcag
     3781 tgctgcaaag gaggcaacgg aggagcagtc tgctctgcca accctcatgt cagtgatgct
     3841 agcaaaacct cggcttgaca cagagcagct ggcgcaaagg ggagctggcc tctgcttcac
     3901 ttttgtttca gctcagcaaa acagtccctc atctacggga tctggcaaca cagagcattc
     3961 ctgcagctcc caaaaacaga tctccatcca gcacagacag acccagtccg acctcacaat
     4021 agaaaaaata tctgcactag aaaacagtaa gaattctgac ttagagaaga aggagggaag
     4081 aatagatgat ttattaagag ccaactgtga tttgagacgg cagattgatg aacagcaaaa
     4141 gatgctagag aaatacaagg aacgattaaa tagatgtgtg acaatgagca agaaactcct
     4201 tatagaaaag tcaaaacaag agaagatggc gtgtagagat aagagcatgc aagaccgctt
     4261 gagactgggc cactttacta ctgtccgaca cggagcctca tttactgaac agtggacaga
     4321 tggttatgct tttcagaatc ttatcaagca acaggaaagg ataaattcac agagggaaga
     4381 gatagaaaga caacggaaaa tgttagcaaa gcggaaacct cctgccatgg gtcaggcccc
     4441 tcctgcaacc aatgagcaga aacagcggaa aagcaagacc aatggagctg aaaatgaaac
     4501 gccctcttct gggaatacag agctaaagga tacagcccca gccttaggag cccacagttt
     4561 acttaggtta acgttagcag aataccatga acaagaagaa atcttcaaac tcagattagg
     4621 tcatcttaaa aaggaggaag cagagatcca ggcagagctg gagagactag aaagggttag
     4681 aaatctacat atcagggaac taaaaaggat acataatgaa gataattcac aatttaaaga
     4741 tcatccaacg ctaaatgaca gatatttgtt gttacatctt ttgggtagag gaggtttcag
     4801 tgaagtttac aaggcatttg atctaacaga gcaaagatac gtagctgtga aaattcacca
     4861 gttaaataaa aactggagag atgagaaaaa ggagaattac cacaagcatg catgtaggga
     4921 ataccggatt cataaagagc tggatcatcc cagaatagtt aagctgtatg attacttttc
     4981 actggatact gactcgtttt gtacagtatt agaatactgt gagggaaatg atctggactt
     5041 ctacctgaaa cagcacaaat taatgtcgga gaaagaggcc cggtccatta tcatgcagat
     5101 tgtgaatgct ttaaagtact taaatgaaat aaaacctccc atcatacact atgacctcaa
     5161 accaggtaat attcttttag taaatggtac agcgtgtgga gagataaaaa ttacagattt
     5221 tggtctttcg aagatcatgg atgatgatag ctacaattca gtggatggca tggagctaac
     5281 atcacaaggt gctggtactt attggtattt accaccagag tgttttgtgg ttgggaaaga
     5341 accaccaaag atctcaaata aagttgatgt gtggtcggtg ggtgtgatct tctatcagtg
     5401 tctttatgga aggaagcctt ttggccataa ccagtctcag caagacatcc tacaagagaa
     5461 tacgattctt aaagctactg aagtgcagtt cccgccaaag ccagtagtaa cacctgaagc
     5521 aaaggcgttt attcgacgat gcttggccta ccgaaaggag gaccgcattg atgtccagca
     5581 gctggcctgt gatccctact tgttgcctca catccgaaag tcagtctcta caagtagccc
     5641 tgctggagct gctattgcat caacctctgg ggcgtccaat aacagttctt ctaattgccc
     5701 aactttcttg tacaaagtgg gcaagcctat ccctaaccct ctcctcggtc tcgattctac
     5761 gtaataacac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     5821 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     5881 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     5941 ttgcccgcct gtaatcaact ttattataca tagttgatat ccagcacagt ggcggccgct
     6001 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6061 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     6601 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     6661 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6721 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6781 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6841 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6901 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6961 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     7021 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     7081 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     7141 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     7201 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7261 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7321 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7381 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7441 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7501 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7561 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7621 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7681 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     7741 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     7801 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7861 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     7921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8101 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8161 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8281 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     8341 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8401 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8461 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8521 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8581 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8641 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8701 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8761 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8821 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8881 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8941 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9001 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9061 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9121 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9181 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9241 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9301 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9361 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9421 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9481 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9541 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9601 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9661 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9721 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9781 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9841 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9901 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9961 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10021 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10081 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10141 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10201 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10261 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10321 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10381 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10441 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10501 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10561 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10621 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10681 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10741 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10801 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10921 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10981 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11041 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11101 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11161 caattaat
//
