LOCUS Exported 2106 bp ds-DNA linear SYN 15-JUN-2021 DEFINITION Protein expression/Streptavidin pull-down. ACCESSION . VERSION . KEYWORDS TBXTA-c028 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2106) TITLE Opher Gileadi SGC expression vectors REFERENCE 2 (bases 1 to 2106) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 15, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2106 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 4..21 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 28..681 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGADHPPK" primer_bind 639..661 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" CDS 709..729 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" CDS 2059..2103 /codon_start=1 /product="peptide tag that allows for enzymatic biotinylation" /label=AviTag(TM) /translation="GLNDIFEAQKIEWHE" ORIGIN 1 atgcaccatc atcatcatca ttcttctatg tcccctatac taggttattg gaaaattaag 61 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat 121 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag 181 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc 241 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag 301 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat 361 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa 421 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct 481 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat 541 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag 601 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt 661 ggtgccgacc atcctccaaa atcgagctca ggtgtagatc tgggtaccga gaacctgtac 721 ttccaatcca tgtccaatag cagccctggc accgaaagcg ccggtaagag cctacagtac 781 cgtgtggacc atttactgag cgccgtggag aacgaactac aggcaggcag cgaaaaaggt 841 gacccgaccg aacgcgaact gcgtgtgggt ctggaagaaa gcgaactgtg gctgcgcttt 901 aaagagctga ccaacgaaat gatcgtgacc aaaaacggcc gccgcatgtt tcctgtgctg 961 aaagttaacg tgagcggcct ggacccgaac gccatgtaca gcttcctgct ggatttcgtt 1021 gccgcagata accaccgctg gaagtatgtg aacggcgaat gggtgccggg tggcaaaccg 1081 gaaccgcagg cacctagctg cgtgtatatc cacccggata gccctaactt cggtgcccat 1141 tggatgaaag ccccggtgag cttcagcaag gtgaagctga ccaacaaact gaacggcggc 1201 ggccaaatca tgctgaacag cttacataaa tatgaacctc gcatccacat tgtgcgtgtg 1261 ggcggcccgc aacgtatgat caccagccat tgcttcccgg agacacagtt cattgccgtt 1321 accgcctacc agaacgagga aattaccgca ctgaaaatta aatacaaccc ttttgcaaaa 1381 gccttcttag atgccaaaga gcgcagcgac cacaaagaaa tgatggagga accgggcgac 1441 agccagcagc ctggttatag ccaatggggc tggctgctgc cgggtaccag tacactgtgc 1501 ccgccggcaa acccgcatcc tcagtttggt ggcgccctga gcttaccgag cacccacagt 1561 tgtgatcgct atcctaccct gcgtagccat cgtagcagtc cgtacccgag cccgtatgca 1621 catcgtaaca acagcccgac atatagcgac aacagcccgg cctgtctgag catgctacag 1681 agccatgata actggagcag cctgggcatg ccggcacatc cgagcatgct gccggtgagt 1741 cacaatgcca gcccgccgac cagtagcagc cagtatccta gcctgtggag cgttagtaac 1801 ggtgcagtga ccccgggtag ccaggcagca gcagttagca atggtctggg cgcccagttt 1861 tttcgcggca gtccggcaca ttacaccccg ctgacccatc ctgtgagtgc cccgagcagt 1921 agtggtagcc cgctgtatga aggtgccgca gccgccaccg acattgttga cagccagtat 1981 gatgccgccg cacagggtcg cctgattgca agctggaccc ctgtgagtcc gccgagcatg 2041 agcagtaaag gtggatacgg cctgaatgat atctttgaag cgcagaagat tgaatggcat 2101 gaatga //