LOCUS       Exported                5859 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses full length human Tafazzin with codon modified for 
            cloning. Transgene flanked by AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG/hTAZ-FL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Wang S, Li Y, Xu Y, Ma Q, Lin Z, Schlame M, Bezzerides VJ, Strathdee
            D, Pu WT
  TITLE     AAV Gene Therapy Prevents and Reverses Heart Failure in A Murine 
            Knockout Model of Barth Syndrome.
  JOURNAL   Circ Res. 2020 Mar 9. doi: 10.1161/CIRCRESAHA.119.315956.
  PUBMED    32146862
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   56..185
                     /label=AAV2 ITR
     promoter        498..775
                     /label=chicken beta-actin promoter
     primer_bind     1055..1077
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1139..1158
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1212..1344
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    2253..2841
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2306..2326)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2724..2735)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2884..3005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2895..2914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2949..2968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3167..3296
                     /label=AAV2 ITR
     primer_bind     complement(3315..3332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3315..3331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3324..3346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3473..3928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3560..3579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3770..3791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3954..4058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4059..4919
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4277..4296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5090..5678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5579..5598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5832..5849
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatcgtacca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      301 ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt
      361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      481 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      541 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      601 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
      661 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      721 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      781 cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      841 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      901 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      961 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg
     1021 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1081 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1141 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattggatcc ccgggtaccg
     1201 gtctctgcag agtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg
     1261 gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca
     1321 tccactttgc ctttctctcc acagggcggc cgcgggtggg gatgcctctg cacgtgaagt
     1381 ggccgttccc cgcggtgccg ccgctcacgt ggaccctcgc cagcagcgtc gtcatgggct
     1441 tggtgggcac ctacagctgc ttctggacca agtacatgaa ccacctgacc gtgcacaaca
     1501 gggaggtgct gtacgagctc atcgagaagc gaggccccgc cacgcccctc atcaccgtgt
     1561 ccaatcacca gtcctgcatg gacgaccctc atctctgggg gattctgaaa ctccgccaca
     1621 tctggaacct gaagttgatg cgttggaccc ctgctgctgc tgacatctgc ttcaccaagg
     1681 agctacactc ccacttcttc agcttgggca agtgtgtgcc tgtgtgccga ggagcagaat
     1741 ttttccaagc agagaatgag gggaaaggtg tactagacac aggcaggcac atgccaggtg
     1801 ctggaaaaag aagagagaaa ggagatggcg tctaccagaa ggggatggac ttcattttgg
     1861 agaagctcaa ccacggggac tgggtgcata tcttcccaga agggaaagtg aacatgagtt
     1921 ccgaatttct gcgtttcaag tggggaatcg ggcgcctgat tgctgagtgt catctcaacc
     1981 ccatcatcct acccctgtgg catgtcggaa tgaatgacgt ccttcctaac agtccgccct
     2041 acttcccccg ctttggacag aaaatcactg tgctgatcgg gaagcccttc agtgccctgc
     2101 ctgtactcga gcggctccga gcggagaaca agtcggctgt ggagatgcgg aaagccctga
     2161 cggacttcat tcaagaggaa tttcagcatc tgaagactca ggcagagcag ctccacaacc
     2221 acctccagcc agggagatag aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2281 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2341 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2401 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2461 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2521 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2581 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2641 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2701 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2761 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2821 ccctttgggc cgcctccccg catcgatacc gtcgacccgg gcggccgctt cgagcagaca
     2881 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2941 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3001 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     3061 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag
     3121 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     3181 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3241 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt
     3301 aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3901 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
