LOCUS       Exported                 656 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber 
            suppression and incorporation of azido-phenylalanine; for transient 
            or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    pAS_4xEcoLeuT(CUA)_AnapRS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 656)
  TITLE     Elsaesser amber suppression piggyBac vectors
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      222..231
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
       61 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
      121 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
      181 tacagatcca agctgtgacc ggcgcctact ctagagctag cgccaccatg gaagagcaat
      241 accggcccga agagatcgag agcaaggtgc agctgcattg ggacgagaag cggaccttcg
      301 aagtgaccga ggacgagagc aaagagaagt actactgctt cagcggccct ccctacccca
      361 gcggcagact gcacatggga cacgtgcgga actacaccat cggcgacgtg atcgccagat
      421 accagcggat gctgggcaag aacgtgctgc agcccatcgg ctgggatgcc tttggactgc
      481 ctgctgaagg cgccgctgtg aagaacaaca cagcccctgc cccctggacc tacgacaata
      541 tcgcctacat gaagaaccag ctgaagatgc tgggattcgg ctacgactgg tcccgcgagc
      601 tggccacctg tacccccgag tactacagat gggagcagaa gttcttcacc gagctg
//
