LOCUS Exported 879 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of azido-phenylalanine; for transient or stable piggyBac-mediated integration. ACCESSION . VERSION . KEYWORDS pAS_4xEcoLeuT(CUA)_AnapRS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 879) TITLE Elsaesser amber suppression piggyBac vectors REFERENCE 2 (bases 1 to 879) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..879 /organism="synthetic DNA construct" /mol_type="other DNA" tRNA complement(197..280) /product="amber suppressor variant of an E. coli leucine tRNA (Wu et al., 2004)" /label=tRNA(CUA) tRNA complement(544..627) /product="amber suppressor variant of an E. coli leucine tRNA (Wu et al., 2004)" /label=tRNA(CUA) ORIGIN 1 ttacttatcg tactagagct tcagcaggaa atttaactaa aatctaattt aaccagcata 61 gcaaatatca tttattccca aaatgctaaa gtttgagata aacggacttg atttccggct 121 gttttgacac tatccagaat gccttgcaga tgggtggggc atgctaaata ctgcagcaag 181 aaaaaccgca cttgtctacc cggagcggga cttgaacccg cacagcgcga acgccgaggg 241 atttagaatc ccttgtgtct accgattcca ccatccgggc gaggtaccca ggcggcgcac 301 aagctatata aacctgaagg aaatctcaac tttacactta ggtcaagtta cttatcgtac 361 tagagcttca gcaggaaatt taactaaaat ctaatttaac cagcatagca aatatcattt 421 attcccaaaa tgctaaagtt tgagataaac ggacttgatt tccggctgtt ttgacactat 481 ccagaatgcc ttgcagatgg gtggggcatg ctaaatactg cagcgataaa aaccgcactt 541 gtctacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt tagaatccct 601 tgtgtctaca gattccacca tccgggcgag gtacccaggc ggcgcacaag ctatataaac 661 ctgaaggaaa tctcaacttt acacttaggt caagttactt atcgtactag agcttcagca 721 ggaaatttaa ctaaaatcta atttaaccag catagcaaat atcatttatt cccaaaatgc 781 taaagtttga gataaacgga cttgatttcc ggctgttttg acactatcca gaatgccttg 841 cagatgggtg gggcatgcta aatactgcag ctccaaggc //