LOCUS Exported 9088 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS GFP-EEA1 wt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9088) AUTHORS Lawe DC, Patki V, Heller-Harrison R, Lambright D, Corvera S TITLE The FYVE domain of early endosome antigen 1 is required for both phosphatidylinositol 3-phosphate and Rab5 binding. Critical role of this dual interaction for endosomal localization. JOURNAL J Biol Chem. 2000 Feb 4;275(5):3699-705. PUBMED 10652369 REFERENCE 2 (bases 1 to 9088) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9088 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 547..556 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 553..1269 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 553..1269 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(598..619) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(859..878) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1206..1227 /label=EGFP-C /note="EGFP, forward primer" CDS 1291..5526 /codon_start=1 /product="human early endosome antigen 1" /label=EEA1 /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC DACFNDLQG" polyA_signal 5816..5937 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5853..5872) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5907..5926 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(5944..6399) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6081..6102) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6293..6312 /label=F1ori-R /note="F1 origin, reverse primer" promoter 6426..6530 /gene="bla" /label=AmpR promoter promoter 6532..6889 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(6555..6575) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 6740..6875 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6802..6821 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6924..7718 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6978..6997) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7588..7607 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(7906..7925) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 7950..7997 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 8326..8914 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8815..8834 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1261 ctgtacaagt ccggactcag atctcgagaa atgttaagga ggattttaca gaggactcct 1321 gggagagttg gctctcaagg ttctgattta gattcatcag caactcctat aaacacagtg 1381 gacgtcaata atgaaagctc ttcagaaggt ttcatatgtc cccagtgtat gaaatctctt 1441 ggatctgctg atgaactttt caaacattat gaagctgttc atgatgctgg taatgactca 1501 ggtcatggag gagagtctaa tcttgctttg aagcgagatg atgtaacact gctcagacaa 1561 gaggtccaag acctacaggc ttcacttaag gaagaaaaat ggtactcgga agaattaaag 1621 aaggaattag aaaaatatca agggctgcag cagcaagagg ccaaacctga tgggttggtg 1681 actgattcat cagcagaact acagtctttg gaacagcaat tagaagaagc ccaaacagaa 1741 aattttaata ttaagcaaat gaaagactta tttgaacaga aagcagccca acttgctact 1801 gaaattgcag atataaagtc aaagtatgat gaagaaagga gtcttcgaga agctgctgaa 1861 caaaaagtga cacgtctgac agaagaatta aacaaagagg caactgtaat tcaagatctg 1921 aagacggaac tgcttcagag acctggtata gaagatgttg ccgtgctaaa gaaagaactg 1981 gtccaagttc aaacactaat ggataacatg accttggaac gtgagcgaga atctgaaaaa 2041 ctcaaagatg aatgcaaaaa attgcagtca caatatgcta gctcagaggc cacaataagc 2101 cagctaagga gtgaacttgc caaaggcccc caggaagttg ctgtatatgt acaggaacta 2161 caaaaactga aaagttcagt taatgaatta acacaaaaaa atcagacctt gacagaaaac 2221 ttgctgaaaa aagaacaaga ctatactaag ttagaggaga aacataatga agaatctgtg 2281 agtaaaaaga atattcaggc aacccttcat caaaaagacc tagattgtca acagcttcag 2341 tcaagattgt ctgcatctga aacctcactg catagaatac atgtagaact aagtgaaaaa 2401 ggagaagcta ctcaaaagct caaagaagaa ttatctgagg tagagaccaa gtaccagcat 2461 ctaaaggcgg agtttaagca gctacaacaa cagagagaag aaaaggagca gcatgggtta 2521 caactccaaa gtgaaattaa tcaattacat agcaaacttc tagagacaga gcgccaacta 2581 ggggaagctc atggtaggct gaaggaacag agacagcttt caagtgaaaa gttgatggat 2641 aaagaacaac aagtggctga tttacaactc aaactttctc ggttagaaga gcagttgaag 2701 gaaaaagtta caaattctac agaattgcag catcaattag ataaaacaaa gcaacagcat 2761 caagaacaac aggctcttca gcaaagcacc acggcaaaac ttcgagaagc tcagaatgat 2821 ttggaacaag ttctacgtca aattggcgac aaggaccaaa agatccagaa ccttgaagct 2881 ttattacaga agagtaaaga aaatatttca ttactagaaa aagaaagaga agatctttat 2941 gcaaaaattc aggctggtga aggagagact gctgttctta accagttaca agaaaaaaac 3001 catacactac aggagcaagt aactcaacta acagagaagc tgaagaatca gtcagaaagt 3061 cataaacaag cccaggagaa tttgcatgac caggtacaag agcagaaggc acatcttaga 3121 gctgcacaag accgtgtcct ttccctagaa actagtgtca atgaattaaa tagtcaatta 3181 aatgaaagca aggagaaggt ctcccagctt gacatacaga ttaaagccaa aaccgaacta 3241 ttactatcag cagaagcagc aaaaactgct caaagagctg atcttcagaa tcatttggac 3301 acagctcaaa atgcattaca agataaacag caggagttaa ataagattac tactcagttg 3361 gatcaggtca ctgcaaagtt acaagacaag caagaacatt gcagtcagct ggaaagtcat 3421 cttaaagaat ataaagagaa atacctctct ttagaacaga aaaccgaaga gctagaaggt 3481 caaattaaga aactagaagc tgatagtctt gaagttaaag caagcaagga gcaggctttg 3541 caagatctac aacagcaaag acagctgaac acagatttag agctcagagc cacagaattg 3601 agtaaacaac ttgaaatgga gaaggaaata gtatccagta caagattgga tctacagaaa 3661 aaatctgaag cccttgaaag tatcaagcaa aagcttacca agcaagagga agaaaaacaa 3721 atcctgaaac aagattttga aactttaagt caagaaacaa agattcagca tgaggaattg 3781 aataacagaa ttcaaacaac agtaacagaa ctacaaaaag tgaaaatgga gaaagaagct 3841 ttaatgacag agctttctac agtaaaggac aaactatcaa aagtttctga ttctttgaaa 3901 aactctaaaa gtgaatttga aaaggagaat cagaaaggaa aagccgctat attagacttg 3961 gaaaaaactt gcaaagaatt aaagcatcaa cttcaagtgc agatggaaaa cacacttaag 4021 gaacagaagg aactgaaaaa gtcacttgaa aaagagaagg aggcttctca tcagttgaaa 4081 ttggaactca attcaatgca ggaacaactt atacaggccc agaatacttt aaaacaaaat 4141 gaaaaagaag agcaacaact tcaggggaac ataaatgagc taaagcaatc aagtgaacag 4201 aagaaaaaac aaattgaagc actccaagga gagcttaaaa ttgctgtttt acagaagaca 4261 gagcttgaga ataaactaca gcagcagtta acacaggcag cccaggaact tgcagcagag 4321 aaagagaaaa tatcagtatt acaaaacaac tatgaaaaaa gtcaggaaac tttcaaacag 4381 cttcaatctg atttctatgg gagggaatct gaacttctag ccaccaggca agatcttaag 4441 tctgtagaag agaagctttc tctagcacag gaggacttga tttcaaacag aaatcaaatt 4501 ggaaatcaaa ataaattgat tcaagaactg aagactgcca aggctacatt ggagcaggat 4561 tcagcaaaga aagaacagca attgcaggag cgatgtaaag cactacaaga cattcagaaa 4621 gaaaagtcac tgaaagaaaa agaactggta aatgagaagt ctaaattggc agagatagaa 4681 gaaattaaat gtagacaaga aaaagaaatc actaaactaa acgaagaact caagtcccac 4741 aaactagaaa gcataaagga gataacaaat cttaaagatg ctaaacagct tctaattcag 4801 cagaaattag aacttcaagg aaaagcggac tccctgaagg cagctgttga acaggagaag 4861 agaaatcagc agatactaaa agaccaggtg aaaaaggaag aagaggagct gaagaaagaa 4921 tttattgaga aagaagctaa gttgcattcc gaaataaaag aaaaggaagt aggaatgaag 4981 aagcatgaag aaaatgaggc taaacttacc atgcagatta cagcattaaa tgaaaactta 5041 ggcactgtga agaaggagtg gcaatctagt caacggagag ttagtgagct tgagaaacaa 5101 acggatgact tacggggtga aattgcagta ttagaagcaa cggttcagaa taatcaagat 5161 gaaaggagag cactactgga aagatgtctt aaaggagaag gtgaaataga aaagcttcaa 5221 accaaagtat tagaattgca aagaaagctg gataatacaa ctgcagcagt gcaggagctg 5281 ggcagagaaa accaatcact tcagatcaaa catacacaag cgttgaatag aaagtgggcc 5341 gaagacaatg aagtacaaaa ctgtatggcc tgtgggaaag gcttttcagt aacagtgaga 5401 cggcatcact gccgacagtg tggaaatatc ttctgtgctg aatgttcagc caaaaatgcc 5461 ttaactcctt cctccaagaa gcctgttcgt gtctgtgatg catgtttcaa tgacttgcaa 5521 ggataatggg ttatcacaac ttcagagtaa tattacacta acattagatt tttaataaat 5581 gtacttaata gaggtcttgg actactattt ggtttggaca ctggttgcaa tactagacca 5641 aataggaatt agtatatttt ggaatgttat ggatatcgcg gccgctggat ccaccggatc 5701 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 5761 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 5821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg 5941 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 6001 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 6061 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 6121 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 6181 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 6241 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 6301 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 6361 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 6421 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 6481 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 6541 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 6601 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 6661 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 6721 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 6781 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 6841 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 6901 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 6961 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 7021 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 7081 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 7141 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 7201 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 7261 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 7321 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 7381 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 7441 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 7501 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 7561 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 7621 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 7681 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 7741 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 7801 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 7861 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 7921 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 7981 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 8041 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 8101 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 8161 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 8221 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 8281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 8341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 8401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 8461 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 8521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 8581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 8641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 8701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 8761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 8821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 8881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 8941 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 9001 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat 9061 tagttcatag cccatatatg gagttccg //