LOCUS       Exported                6591 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AAV vector to drive the expression of C1V1-eYFP in PV cortical 
            interneuronsunder the control of the E2 regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-S5E2-C1V1-eYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Vormstein-Schneider DC, Lin JD, Pelkey KA, Chittajallu R, Guo B, 
            Arias Garcia M, Sakopoulos S, Stevenson O, Schneider G, Zhang Q, 
            Sharma J, Franken TP, Smith J, Vogel I, Sanchez V, Ibrahim LA, 
            Burbridge T, Favuzzi E, Saldi GA, Xu Q, Guo L, Yuan X, Zaghloul KA, 
            Sabri E, Goldberg EM, Devinsky O, Batista-Brito R, Reynolds J, Feng 
            G, Fu Z, McBain CJ, Fishell GJ, Dimidschstein J
  TITLE     Viral manipulation of functionally distinct interneurons in mice, 
            non-human primates and humans
  JOURNAL   Nature Neuroscience (2020)
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(142..161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(821..1681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1444..1463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1682..1786)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1854..1872
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1910..1932)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2032..2051
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2068..2523)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2205..2226)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2417..2436
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2598..2738
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2609..2738
                     /label=AAV2 ITR
     primer_bind     complement(2788..2807)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    2817..2898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2842..2861
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    3067..3655
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3173..3184
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3582..3602
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3680..4396)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3725..3746)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4074..4093
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4333..4354
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          5526..5658
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     repeat_region   6462..6591
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
     2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata gaccgaaatc ggcaaaatcc
     2161 cttataaatc aaaagaatag cccgagatag agttgagtgt tgttccagtt tggaacaaga
     2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctaaggcg ctggcaagtg
     2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgctcgg tccgcacgtg
     2761 catgcggtat cgataagctg atacattgat gagtttggac aaaccacaac tagaatgcag
     2821 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2881 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2941 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     3001 gatctagagt cgcggccagc ttgatggccg ctatcacaga tctctcgagc agcgctcggt
     3061 atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     3121 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     3181 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca taggcgagca
     3241 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     3301 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     3361 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     3421 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     3481 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     3541 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     3601 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga
     3661 taagcttgat atcgaattct tacttgtaca gctcgtccat gccgagagtg atcccggcgg
     3721 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     3781 cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     3841 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     3901 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     3961 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     4021 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     4081 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     4141 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt
     4201 agccgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     4261 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     4321 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     4381 cctcgccctt gctcaccacg ttgatgtcga tctggtccag ggggatgtac tcgccctcgc
     4441 tggtgatcct gctcttggcg gccgcgtcct cctcttcagc caccagtgtc tcaacctcca
     4501 tctcttggcc ggcgatcgta attttctgct tctttctgat gtcgccatac aacaggatgt
     4561 gctcgtggat tttgacgcgc aggtaattcc ccagcacacc ccacatgttc tttgcaatca
     4621 ggtcgaggat ggagtgccca atggcagatc cataagggct gatatgaccg aatccttccg
     4681 tgcccagcag aaacaggact ggaaacattc cccaagccac aaagaatgtc caggccatca
     4741 ctcggaccag ttctcgacaa atcccttttg gaactgtgtg gaatgcttcg atataaactt
     4801 tagcagcatg aaaataggta tacataccat agctcaatga gatgaggaag aacaggatct
     4861 tcgtccaccc cgtacacatg gcgctggttg cgccccacac gatgcatccc acatcagaaa
     4921 ccaacaggcc catagtcctt ttgctataat cgtccttcag gcctgtgagg ttgctcagat
     4981 ggatcagaag gaccgggcag gtgagcagcc aggtcgcgta acgaagccac acggtcttgt
     5041 tgccgttgga tgagtagatc accgcaggtt cgtcaaactc atggaaatac tcgatgatga
     5101 acttgatcat ctcgatcgtg gccacgtaaa tggtctccca gccgcaagta gacttccagg
     5161 tctggtagcc gtagaacatc aggcagagcg ctgaaagcgc aaaagtaatc cactgcagaa
     5221 tgttggcagc caacttctcg gcatttgttc cgttggattt aagccaagcc aagcagaagc
     5281 actggccgtt gttcgggatg caaataacag aaccattgtt ctcaagagtg tatgaggttt
     5341 ggaagagcat gcgctcgtgg gcacggtgga aaacgtagtc ggggccatcc tgagtcgcga
     5401 ccggcaccgt cgcgtcactg ccagtcgagg ctcctgcgct gccggccgcc agcgccactg
     5461 ccagcgctag ggcaagaagc catggcctcc gcgacatggt ggcctaccgg tacccagcga
     5521 gctcgctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg cctatcagaa
     5581 acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt aaacctgtct
     5641 tgtaaccttg atacttacgt gcccagtgcc tcacgaccaa cttctgcagc ttaaggcgcc
     5701 gcgctctgct tctggaaggc tgcgctcctc gacttgcagg ctagaagcaa atgtaagcaa
     5761 tagatggctc tgccctgact tttatgccca gcccgggctg caggaagttt aaaataactc
     5821 tgagcaattg agactaaatg cctctgtata gagaaaagac tgccacagac aggaatgttc
     5881 ttggtgtctc ttgggtgcac tgttccatca taccatgtgt cccatatagt gctttcatat
     5941 agattgtaaa tactgcatta ttttaaggaa atcatagtta attaaggtaa gtatttccaa
     6001 aatggggtta taacaattta ctccaacatc acatgttaat atgatctgag ggaattatgc
     6061 atagtgtttg ccttttgttt ttgtttattt ttccattact gtttcatagc aatgaagaga
     6121 caaatgctga ctctagcaga atttcctcag tctgctaagt atagctacat aattgccacc
     6181 ttcaccagca ccattgggaa aaaaataacc tacattactt gactccatat agctatattg
     6241 ccataacttt gtagggctta cagtaatgtg gtttctgtat aaaactgaat tgaaatctca
     6301 gaattctaca gagagaggaa tggggacaag aaaatgagga gggaactgaa gtggcttgtg
     6361 tcaaagcctt cttttaggaa gtgtgcactt aacttctagt cagtaagcta tagcagccat
     6421 gttagattaa accactagtt taattaaacg cgtgcggccg caggaacccc tagtgatgga
     6481 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     6541 ccgacgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g
//
