LOCUS Exported 5927 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral expression of H-RAS-V12. ACCESSION . VERSION . KEYWORDS pBABEneo-HRASV12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5927) AUTHORS Lock R, Roy S, Kenific CM, Su JS, Salas E, Ronen SM, Debnath J TITLE Autophagy facilitates glycolysis during Ras-mediated oncogenic transformation. JOURNAL Mol Biol Cell. 2011 Jan 15;22(2):165-78. doi: 10.1091/mbc.E10-06-0500. Epub 2010 Nov 30. PUBMED 21119005 REFERENCE 2 (bases 1 to 5927) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5927 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 942..1358 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1288..1310 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1326..1342 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(2066..2086) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2071..2400 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2251..2386 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2313..2332 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2757..3551 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2811..2830) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3421..3440 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 4074..4093 /label=pBR322ori-F /note="pBR322 origin, forward primer" LTR 4355..4824 /note="long terminal repeat from Moloney murine leukemia virus" CDS complement(4939..5799) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5562..5581 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5800..5904) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt 901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc 961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg 1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg 1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg 1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag 1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc 1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat 1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac cgacttctac 1381 ctaaagagat ctgccaccat gactgaatac aagcttgtgg tggtgggcgc tgtaggcgtg 1441 ggaaagagtg ccctgaccat ccagctgatc cagaaccact ttgtggacga gtatgatccc 1501 actatagagg actcctaccg gaaacaggtg gtcattgatg gggagacatg tctactggac 1561 atcttagaca cagcaggtca agaagagtat agtgccatgc gggaccagta catgcgcaca 1621 ggggagggct tcctctgtgt atttgccatc aacaacacca agtccttcga ggacatccat 1681 cagtacaggg agcagatcaa gcgggtgaaa gattcagatg atgtgccaat ggtgctggtg 1741 ggcaacaagt gtgacctggc tgctcgcact gttgagtctc ggcaggccca ggaccttgct 1801 cgcagctatg gcatccccta cattaaaaca tcagccaaga cccggcaggg cgtggaggat 1861 gccttctata cactagtccg tgagattcgg cagcataaat tgcggaaact gaacccaccc 1921 gatgagagtg gtcctggctg catgagctgc aaatgtgtgc tgtcctgaca ccaggctcag 1981 gacatggagg tgccggatgc agggtggaca tgacccaggg ctcatctttg ggatcggaat 2041 tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 2101 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 2161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2221 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 2281 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 2341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 2401 aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt 2461 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct 2521 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc 2581 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc 2641 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga 2701 tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga 2761 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 2821 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 2881 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 2941 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 3001 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 3061 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 3121 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 3181 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 3241 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 3301 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 3361 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 3421 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 3481 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 3541 agttcttctg agcgggactc tggggttcgc atcgatgcat cgatgcggcg ggtgtggtgg 3601 ttacgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 3661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 3781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 3841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 3901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 3961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 4021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 4141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcatc gataaaataa 4201 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 4261 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag 4321 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct 4381 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg 4441 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt 4501 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc 4561 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc 4621 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg 4681 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt 4741 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga 4801 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt 4861 cttaagtatt tacattaaat ggccatagtg cggccgcaaa tcaatctaaa gtatatatga 4921 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 4981 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 5041 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 5101 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 5161 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 5221 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 5281 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 5341 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 5401 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 5461 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 5521 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 5581 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 5641 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 5701 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 5761 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 5821 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 5881 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac //