LOCUS Exported 12072 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION 3rd generation lentiviral expression of untagged Casilio-ME2.1 effector PUFa-hGADD45A-hTET1(CD) in mammalian cells. ACCESSION . VERSION . KEYWORDS pAT360_CMV/CAG_PUFa-hGADD45A-hTET1(CD) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12072) AUTHORS Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li S, Cheng AW TITLE Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways. JOURNAL Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7. PUBMED 31541098 REFERENCE 2 (bases 1 to 12072) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12072 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 743..760 /label=L4440 /note="L4440 vector, forward primer" protein_bind 877..898 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 913..943 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 951..967 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 956..978 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 975..991 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 975..991 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 1010..1030 /label=T3 /note="T3 promoter, forward primer" promoter 1012..1030 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 1058..1284 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 1285..1465 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1512..1637 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 2130..2363 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2548..2592 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2767..3267 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 3186..3204 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 3289..3888 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3289..3308) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3785..3805 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 3930..4047 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4164..4467 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4468..4671 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4622..4642 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 4694..4718 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4769..4789 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4793..4813 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4817..4837 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4928..4948 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6005..6025 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6029..6049 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind complement(8873..8897) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 8899..8940 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 8982..9570 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9035..9055) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9453..9464) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9642..9875 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9953..10074 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9990..10009) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10044..10063 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 10114..10249 /label=SV40 ori /note="SV40 origin of replication" primer_bind 10176..10195 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(10269..10288) /label=T7 /note="T7 promoter, forward primer" promoter complement(10270..10288) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(10298..10315) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(10298..10314) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10307..10329) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 10456..10911 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10543..10562) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10753..10774 /label=F1ori-F /note="F1 origin, forward primer" promoter 10937..11041 /gene="bla" /label=AmpR promoter CDS 11042..11902 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(11260..11279) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc 1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg 1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac 1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag 1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt 1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag 1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga 1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg 1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag 1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc 1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg 1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa 1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata 1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag 1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg 1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa 2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg 2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca 2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg 2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg 2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga 2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc 2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac 2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta 2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa 2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc 2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg 2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg 2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac 3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg 3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc 3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac 3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt 4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt 4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt 4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt 4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg 4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta 4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac 4741 catgatcgat ggcggaggcg gatctgatcc aaaaaagaag agaaaggtag atccaaaaaa 4801 gaagagaaag gtagatccaa aaaagaagag aaaggtagga tctaccggat ctagaaacga 4861 tggtggtggt ggaagcgggg gtgggggcag cggtggaggg ggaagcgggc gcgccgggat 4921 cctccccccc aagaaaaaga ggaaggtatc tagaggccgc agccgccttt tggaagattt 4981 tcgaaacaac cggtacccca atttacaact gcgggagatt gctggacata taatggaatt 5041 ttcccaagac cagcatgggt ccagattcat tcagctgaaa ctggagcgtg ccacaccagc 5101 tgagcgccag cttgtcttca atgaaatcct ccaggctgcc taccaactca tggtggatgt 5161 gtttggtaat tacgtcattc agaagttctt tgaatttggc agtcttgaac agaagctggc 5221 tttggcagaa cggattcgag gccacgtcct gtcattggca ctacagatgt atggcagccg 5281 tgttatcgag aaagctcttg agtttattcc ttcagaccag cagaatgaga tggttcggga 5341 actagatggc catgtcttga agtgtgtgaa agatcagaat ggcaatcacg tggttcagaa 5401 atgcattgaa tgtgtacagc cccagtcttt gcaatttatc atcgacgcgt ttaagggaca 5461 ggtatttgcc ttatccacac atccttatgg ctgccgagtg attcagagaa tcctggagca 5521 ctgtctccct gaccagacac tccctatttt agaggagctt caccagcaca cagagcagct 5581 ggtacaggat caatatggaa attatgtaat ccaacatgta ctggagcacg gtcgtcctga 5641 ggataaaagc aaaattgtag cagaaatccg aggcaatgta cttgtattga gtcagcacaa 5701 atttgcaagc aatgttgtgg agaagtgtgt tactcacgcc tcacgtacgg agcgcgctgt 5761 gctcatcgac gaggtgtgca ccatgaacga cggtccccac agtgccttat acaccatgat 5821 gaaggaccag tatgccaact acgtggtcca gaagatgatt gacgtggcgg agccaggcca 5881 gcggaagatc gtcatgcata agatccggcc ccacatcgca actcttcgta agtacaccta 5941 tggcaagcac attctggcca agctggagaa gtactacatg aagaacggtg ttgacttagg 6001 ggacccaaag aagaagcgca aagtggatcc taaaaagaaa agaaaggtag gcggccgcgg 6061 cggaggttct ggtggaggat caggtggagg gagtggtgga ggaagtgggg gaggttctgg 6121 aggtggatcg ttgactttgg aggaattctc ggctggagag cagaagaccg aaaggatgga 6181 taaggtgggg gatgccctgg aggaagtgct cagcaaagcc ctgagtcagc gcacgatcac 6241 tgtcggggtg tacgaagcgg ccaagctgct caacgtcgac cccgataacg tggtgttgtg 6301 cctgctggcg gcggacgagg acgacgacag agatgtggct ctgcagatcc acttcaccct 6361 gatccaggcg ttttgctgcg agaacgacat caacatcctg cgcgtcagca acccgggccg 6421 gctggcggag ctcctgctct tggagaccga cgctggcccc gcggcgagcg agggcgccga 6481 gcagcccccg gacctgcact gcgtgctggt gacgaatcca cattcatctc aatggaagga 6541 tcctgcctta agtcaactta tttgtttttg ccgggaaagt cgctacatgg atcaatgggt 6601 tccagtgatt aatctccctg aacggtctag aggccgcggg ggaggcggtt ccggtggcgg 6661 cggaagcgga ggtggaggat cagggccggc cgaactgccc acctgcagct gtcttgatcg 6721 agttatacaa aaagacaaag gcccatatta tacacacctt ggggcaggac caagtgttgc 6781 tgctgtcagg gaaatcatgg agaataggta tggtcaaaaa ggaaacgcaa taaggataga 6841 aatagtagtg tacaccggta aagaagggaa aagctctcat gggtgtccaa ttgctaagtg 6901 ggttttaaga agaagcagtg atgaagaaaa agttctttgt ttggtccggc agcgtacagg 6961 ccaccactgt ccaactgctg tgatggtggt gctcatcatg gtgtgggatg gcatccctct 7021 tccaatggcc gaccggctat acacagagct cacagagaat ctaaagtcat acaatgggca 7081 ccctaccgac agaagatgca ccctcaatga aaatcgtacc tgtacatgtc aaggaattga 7141 tccagagact tgtggagctt cattctcttt tggctgttca tggagtatgt actttaatgg 7201 ctgtaagttt ggtagaagcc caagccccag aagatttaga attgatccaa gctctccctt 7261 acatgaaaaa aaccttgaag ataacttaca gagtttggct acacgattag ctccaattta 7321 taagcagtat gctccagtag cttaccaaaa tcaggtggaa tatgaaaatg ttgcccgaga 7381 atgtcggctt ggcagcaagg aaggtcgtcc cttctctggg gtcactgctt gcctggactt 7441 ctgtgctcat ccccacaggg acattcacaa catgaataat ggaagcactg tggtttgtac 7501 cttaactcga gaagataacc gctctttggg tgttattcct caagatgagc agctccatgt 7561 gctacctctt tataagcttt cagacacaga tgagtttggc tccaaggaag gaatggaagc 7621 caagatcaaa tctggggcca tcgaggtcct ggcaccccgc cgcaaaaaaa gaacgtgttt 7681 cactcagcct gttccccgtt ctggaaagaa gagggctgcg atgatgacag aggttcttgc 7741 acataagata agggcagtgg aaaagaaacc tattccccga atcaagcgga agaataactc 7801 aacaacaaca aacaacagta agccttcgtc actgccaacc ttagggagta acactgagac 7861 cgtgcaacct gaagtaaaaa gtgaaaccga accccatttt atcttaaaaa gttcagacaa 7921 cactaaaact tattcgctga tgccatccgc tcctcaccca gtgaaagagg catctccagg 7981 cttctcctgg tccccgaaga ctgcttcagc cacaccagct ccactgaaga atgacgcaac 8041 agcctcatgc gggttttcag aaagaagcag cactccccac tgtacgatgc cttcgggaag 8101 actcagtggt gccaatgcag ctgctgctga tggccctggc atttcacagc ttggcgaagt 8161 ggctcctctc cccaccctgt ctgctcctgt gatggagccc ctcattaatt ctgagccttc 8221 cactggtgtg actgagccgc taacgcctca tcagccaaac caccagccct ccttcctcac 8281 ctctcctcaa gaccttgcct cttctccaat ggaagaagat gagcagcatt ctgaagcaga 8341 tgagcctcca tcagacgaac ccctatctga tgaccccctg tcacctgctg aggagaaatt 8401 gccccacatt gatgagtatt ggtcagacag tgagcacatc tttttggatg caaatattgg 8461 tggggtggcc atcgcacctg ctcacggctc ggttttgatt gagtgtgccc ggcgagagct 8521 gcacgctacc actcctgttg agcaccccaa ccgtaatcat ccaacccgcc tctcccttgt 8581 cttttaccag cacaaaaacc taaataagcc ccaacatggt tttgaactaa acaagattaa 8641 gtttgaggct aaagaagcta agaataagaa aatgaaggcc tcagagcaaa aagaccaggc 8701 agctaatgaa ggtccagaac agtcctctga agtaaatgaa ttgaaccaaa ttccttctca 8761 taaagcatta acattaaccc atgacaatgt tgtcaccgtg tccccttatg ctctcacaca 8821 cgttgcgggg ccctataacc attgggtcat cgattgatta attaagaatt cgacccagct 8881 ttcttgtaca aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg 8941 tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca 9001 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 9061 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 9121 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 9181 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 9241 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 9301 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 9361 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga 9421 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 9481 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 9541 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc 9601 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 9661 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 9721 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 9781 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 9841 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct 9901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 9961 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 10021 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 10081 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 10141 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 10201 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac 10261 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc 10321 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 10381 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 10441 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 10501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 10561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 10621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 10681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 10741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 10801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 10861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac 10921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 10981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 11041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 11101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 11161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 11221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 11281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 11341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 11401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 11461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 11521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 11581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 11641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 11701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 11761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 11821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 11881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 11941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 12001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 12061 caaaggatct tc //