LOCUS Exported 5775 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mEGFP-CREB donor for G-CREB. ACCESSION . VERSION . KEYWORDS CMV-mEGFP-CREB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5775) AUTHORS Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y, Chu J, Yasuda R TITLE In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain. JOURNAL Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 10.1016/j.neuron.2019.11.028. PUBMED 31883788 REFERENCE 2 (bases 1 to 5775) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5775 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 613..1329 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(658..679) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(919..938) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1266..1287 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2374..2439 /label=MCS /note="multiple cloning site" polyA_signal 2563..2684 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2600..2619) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2654..2673 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2691..3146) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2828..2849) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3040..3059 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3173..3277 /gene="bla" /label=AmpR promoter promoter 3279..3636 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3302..3322) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3487..3622 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3549..3568 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3671..4465 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3725..3744) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4335..4354 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4653..4672) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4697..4744 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5073..5661 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5562..5581 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagc ctacccctac ccctaccatg accatggact ctggagcaga caaccagcag 1381 agtggagatg ctgctgtaac agaagctgaa agtcaacaaa tgacagttca agcccagcca 1441 cagattgcca cattagccca ggtatccatg ccagcagctc atgcgacgtc atctgctccc 1501 actgtaacct tagtgcagct gcccaatggg cagacagtcc aggtccatgg ggtcatccag 1561 gcggcccagc catcagttat tcagtctcca caagtccaaa cagttcagtc ttcctgtaag 1621 gacttaaaaa gacttttctc cggaactcag atttcaacta ttgcagaaag tgaagattca 1681 caggagtctg tggatagtgt aactgattcc caaaaacgaa gggaaatcct ttcaaggagg 1741 ccttcctaca ggaaaatttt gaatgactta tcttctgatg caccaggggt gccaaggatt 1801 gaagaagaaa aatcagaaga agagacttca gcccctgcca tcaccactgt aacagtgcca 1861 accccgattt accaaactag cagtgggcag tatattgcca ttacccaggg aggagcaata 1921 cagctggcta acaatggtac cgatggggta cagggcctgc agacattaac catgaccaat 1981 gcagctgcca ctcagccggg tactaccatt ctacaatatg cacagaccac tgatggacag 2041 cagattctag tgcccagcaa ccaagttgtt gttcaagctg cctctggtga tgtacaaaca 2101 taccagattc gcacagcacc cactagcacc attgcccctg gagttgttat ggcgtcctcc 2161 ccagcacttc ctacacagcc tgctgaagaa gcagcacgaa agagagaggt tcgtctaatg 2221 aagaacaggg aagcagcaag agaatgtcgt agaaagaaga aagaatatgt gaaatgttta 2281 gagaacagag tggcagtgct tgaaaaccaa aacaaaacat tgattgagga gctaaaagca 2341 cttaaggacc tttactgcca caagtcagat tgatccggac tcagatctcg agctcaagct 2401 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc 2461 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 2521 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 2581 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2641 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag 2701 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 2761 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 2821 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 2881 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 2941 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 3001 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 3061 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 3121 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac 3181 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 3241 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg 3301 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3361 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3481 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3541 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 3601 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg 3661 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3841 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3901 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 4021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 4081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 4141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag 4201 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4441 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4501 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4561 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4621 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa 4681 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt 4741 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc 4801 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt 4861 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac 4921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 4981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 5041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 5101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 5161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 5221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 5281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 5341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 5401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 5461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 5521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 5581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 5641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 5701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 5761 attaccgcca tgcat //