LOCUS       Exported               11698 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Destination vector for doxycycline-inducible expression from plasmid
            hosting piggyBac hygromycin cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    pAT1501-[PB-4xHSab-EF1a-Hygro_2A_rtTA3-SV40pA(-)_pCW-DEST]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   299..333
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1485..1566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1522..1541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1576..1595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1603..2349)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(2350..2403)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2413..3435)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3465..3489)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3550..4728)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3559..4497
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3582..3602)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4745..5059
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4753..4771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4789..4807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4824..4842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4860..4878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4896..4914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4931..4949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4967..4985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5032..5056
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5084..5208
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5233..5263
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5317..5976
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5383..5402)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6318..6623
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6664..6788)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6841..6882
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6920..6937)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6926..7150
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7970..8032
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8070..8089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8071..8089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8096..8113)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8096..8112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8105..8127)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8250..8552
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             8901..9695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8955..8974)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9565..9584
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(9945..10805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10568..10587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10929..11517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11418..11437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11671..11688
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttttaacc
      301 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
      361 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
      421 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
      481 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
      541 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
      601 agatatcaac tagaatgcta gacctttcgt cttcaagaat tccgatcata ttcaataacc
      661 cttaattagg tccctcgaag aggttcactg gcgcgttgga tccccgggta ccgagttggg
      721 agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc
      781 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc
      841 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc
      901 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag
      961 ctttaggctg aaagagagat ttagaatgac agtctagtgg gagctcacgg ggacagcccc
     1021 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc
     1081 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg
     1141 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
     1201 tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga
     1261 tttagaatga cagaactcga tttcattgca gactggcgcg ccgccttttt acggttcctg
     1321 gccttttgct ggccttttgc tcacatgtca cgtgaggcct taacgtctcg ccctttggtc
     1381 tccccctctt aagtaccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     1441 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     1501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1561 cactgcattc tagttgtggt ttgtccaaac tcatcggcta gcttacccgg ggagcatgtc
     1621 aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc
     1681 atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc cgccgctttc
     1741 gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg
     1801 ttcggctccc tgccggtcga acagctcaat tgcttgtctc agaagtgggg gcatagaatc
     1861 ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca
     1921 gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc
     1981 ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg
     2041 ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggtatgactt
     2101 ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcaga agtgggtatg
     2161 atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg
     2221 ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc
     2281 ttcgataccg acaccattga gtaattccag agctccgttt atgactttgc tcttgtccag
     2341 tctagacatt ggaccagggt tttcttcaac atcaccacaa gtgaggagag aacctctacc
     2401 ttcggcaccg ggttcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
     2461 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
     2521 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
     2581 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
     2641 atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
     2701 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
     2761 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
     2821 caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc
     2881 ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat
     2941 cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt
     3001 agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc
     3061 ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg gcagttcggt
     3121 ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct
     3181 ctcgctaaat tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg
     3241 ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata
     3301 tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat
     3361 caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag
     3421 ttcaggcttt ttcatggtgg cggcactagt aagggcgaat tcggagcctg cttttttgta
     3481 caaacttgtt gatatctgca gaattccacc acactggact agtggatccg agctcggtac
     3541 caagcttctt cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt
     3601 gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg
     3661 ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa
     3721 cccctccccc caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct
     3781 ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct
     3841 tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga
     3901 gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg
     3961 ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc
     4021 aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg
     4081 cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac
     4141 cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag
     4201 cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca
     4261 ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac
     4321 tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc
     4381 aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac
     4441 gcaagggcca taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg
     4501 tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata
     4561 cggttctccc ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt
     4621 taccccgcgc caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc
     4681 ggggactgtg ggcgatgtgc gctctgccca ctgacgggca ccggagcctc acgatcgata
     4741 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     4801 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     4861 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     4921 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     4981 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     5041 agtgaaccgt cagatcgcct ggagaattgg ctaggcaccg gtgacaagtt tgtacaaaaa
     5101 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     5161 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     5221 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     5281 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     5341 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     5401 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     5461 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     5521 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     5581 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     5641 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     5701 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     5761 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     5821 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     5881 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     5941 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     6001 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     6061 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     6121 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     6181 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     6241 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     6301 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     6361 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     6421 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     6481 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     6541 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     6601 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     6661 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     6721 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     6781 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     6841 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttaggggccc
     6901 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6961 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7021 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7081 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7141 ggctctatgg cgacgcgcct ggatccccgg gtaccgagtt gggagctcac ggggacagcc
     7201 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     7261 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     7321 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     7381 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga
     7441 gatttagaat gacagtctag tgggagctca cggggacagc ccccccccaa agcccccagg
     7501 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     7561 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
     7621 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
     7681 acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaact
     7741 cgatttcatt gcagactggc cggccactag tacgcgccgg ctcgacatac tagttaaaag
     7801 ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca
     7861 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
     7921 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
     7981 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaaaagg
     8041 gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga gtcgtattac aattcactgg
     8101 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8161 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8221 cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga
     8281 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga
     8341 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     8401 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     8461 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     8521 acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga
     8581 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     8641 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     8701 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     8761 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     8821 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     8881 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     8941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     9001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     9061 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     9121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     9181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     9241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     9301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     9361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     9421 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     9481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     9541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     9601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     9661 tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga
     9721 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     9781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9841 ccgctcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgacc
    10861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    11101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
