LOCUS Exported 11944 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression . ACCESSION . VERSION . KEYWORDS Tet1-dCas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11944) TITLE Aberrant promoter methylation contributes to LRIG1 silencing in basal/triple negative breast cancer JOURNAL Unpublished REFERENCE 2 (bases 1 to 11944) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11944 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" CDS 702..722 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 741..806 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 2916..7019 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 7095..7142 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 7161..7217 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(7281..7300) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 7325..7373 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 7575..8003 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7662..7681) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7872..7893 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8012..8032) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8017..8346 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8197..8332 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8259..8278 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 8413..9207 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8467..8486) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9077..9096 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 9383..9504 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9420..9439) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9474..9493 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9553..9569) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9553..9569) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9566..9588) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9577..9593 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9601..9631) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9646..9667 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9784..9801) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9955..10543) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10035..10054) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10714..11574) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11337..11356 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11575..11679) /gene="bla" /label=AmpR promoter primer_bind complement(11754..11773) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg gactctagag gatcgaaccc ttgccaccat gcccaagaag aagaggaagg 721 tgggaggctc cggaggaagc gactataagg accacgacgg agactacaag gatcatgata 781 ttgattacaa agacgatgac gataagggtg gcgggtccgg cggtggatcc ggtaccgaag 841 ctgcaccctg tgactgtgat ggaggtacac aaaaagaaaa aggcccatat tatacacacc 901 ttggggcagg accaagtgtg gctgctgtca gggagctcat ggagactagg tttggccaga 961 aggggaaggc aatccggatt gagaagatag tgttcacggg gaaggaaggg aagagctctc 1021 agggctgccc ggtcgccaag tgggtgatca gaagaagtgg tcctgaagag aagcttattt 1081 gtttggttcg tgagcgtgta gaccatcact gttcgacggc tgtgatagtt gtccttatcc 1141 tgctgtggga aggtatccct cgcctgatgg ctgaccgcct gtacaaagag ctcactgaga 1201 acttgaggtc ctacagcgga catcccacag accgaagatg taccctcaac aaaaagcgta 1261 cctgcacctg tcaaggcatc gacccaaaaa cctgcggagc gtccttctcc tttggctgtt 1321 cgtggagcat gtatttcaac ggctgtaagt ttgggaggag tgaaaacccc agaaaattca 1381 gacttgctcc aaactacccc ttacataact actataagag aattactgga atgagttctg 1441 aaggaagtga cgtgaaaacc gggtggatca ttccagaccg caagaccctc ataagcagag 1501 aggaaaaaca gcttgaaaag aatttacaag aattggctac agtattagct ccactttaca 1561 agcagatggc tccagttgct tatcaaaatc aggtggaata tgaagaagtt gctggagact 1621 gtcgacttgg aaatgaagag gggcgtcctt tctctggtgt cacctgttgc atggattttt 1681 gtgcccattc tcacaaggac attcacaaca tgcacaacgg aagcaccgtg gtgtgtacgt 1741 tgattcgagc agatggccgt gacacaaatt gtcccgagga tgaacaactc cacgtcctgc 1801 cactataccg gcttgcagac actgatgaat ttggctccgt ggaagggatg aaggccaaaa 1861 tcaaatctgg ggccatccaa gtcaatgggc caaccaggaa gaggcgacta cgttttactg 1921 agcctgttcc tcgatgtggg aagagggcca aaatgaagca gaaccacaat aaatcaggtt 1981 cacacaacac taagagcttt tcatcagcct catctacttc tcacctagtg aaagacgaat 2041 ctacagactt ctgtcccctg caggcttcct ccgcagaaac atctacctgt acgtacagta 2101 aaacagcctc aggtgggttt gcagaaacaa gtagtattct ccactgcaca atgccttctg 2161 gagcacacag tggtgctaat gcagctgctg gggaatgtac tggaacggtg cagcctgccg 2221 aggtggctgc tcatcctcac cagtctcttc ccacagccga ttctcccgtt catgctgagc 2281 ctctcactag tccatctgag cagctaactt ctaaccagtc aaaccagcag ctccctctcc 2341 tcagcaattc tcagaaactg gcttcctgtc aggtggaaga tgagcggcac cctgaagcgg 2401 atgagcctca gcaccccgag gacgataact tgcctcaact tgatgaattc tggtcagaca 2461 gtgaggagat ctacgccgat ccttcctttg gtggcgtggc gatagcaccc attcacggct 2521 cggtgctcat tgagtgcgct cggaaggagc ttcatgctac cacctctttg cgctccccca 2581 aacgaggggt cccttttcgt gtgtcccttg tattctacca gcacaaaagc ctaaacaagc 2641 ctaatcatgg ttttgatatc aacaaaatta agtgtaaatg caaaaaagta acgaaaaaaa 2701 agcccgcaga ccgggagtgt cctgatgtat cccccgaagc caatttatca caccaaattc 2761 cttctcgagt tgcatcaacc ttaacccgag acaatgttgt taccgtgtcc ccatactctc 2821 tcactcatgt tgcgggaccc tacaatcgtt gggtcagtac cggagggagt ggtggaagcg 2881 gcggttctgg tggctcaggc ggcagcggcc ggccaatgga caagaagtac tccattgggc 2941 tcgctatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac aaggtgccga 3001 gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg 3061 gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac 3121 ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg 3181 agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg 3241 aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc 3301 atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg 3361 ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc 3421 tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg 3481 ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca 3541 aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc 3601 tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca ctcgggctga 3661 cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag 3721 acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag tacgcagacc 3781 tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt ctgcgagtga 3841 acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat gatgagcacc 3901 accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag aagtacaagg 3961 aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc ggagcaagcc 4021 aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc accgaggagc 4081 tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa 4141 gcatccccca ccagattcac ctgggcgaac tgcacgctat cctcaggcgg caagaggatt 4201 tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca tttcggatac 4261 cctactatgt aggccccctc gcccggggaa attccagatt cgcgtggatg actcgcaaat 4321 cagaagagac catcactccc tggaacttcg aggaagtcgt ggataagggg gcctctgccc 4381 agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa aaggtgcttc 4441 ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc aaggtcaaat 4501 acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag aaagctatcg 4561 tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa gaagactatt 4621 tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat cgcttcaacg 4681 catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac ttcctggaca 4741 atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg tttgaagata 4801 gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac aaagtcatga 4861 aacagctcaa gaggcgccga tatacaggat gggggcggct gtcaagaaaa ctgatcaatg 4921 ggatccgaga caagcagagt ggaaagacaa tcctggattt tcttaagtcc gatggatttg 4981 ccaacaggaa cttcatgcag ttgatccatg atgactctct cacctttaag gaggacatcc 5041 agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct aatcttgcag 5101 gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca 5161 aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa 5221 ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa 5281 aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg 5341 agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg 5401 acatcaatcg gctctccgac tacgacgtgg atgctatcgt gccccagtct tttctcaaag 5461 atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata 5521 acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg 5581 ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt 5641 ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca 5701 agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa aatgacaaac 5761 tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat ttcagaaagg 5821 actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat gatgcctacc 5881 tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagcttgaa tctgaatttg 5941 tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa 6001 taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt ttcaagaccg 6061 agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca aacggagaaa 6121 caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccggaag gtcctgtcca 6181 tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc tccaaggaaa 6241 gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat tgggacccca 6301 agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt gtggccaaag 6361 tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc atcacaatca 6421 tggagcgatc aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa ggatataaag 6481 aggtcaaaaa agacctcatc attaagcttc ccaagtactc tctctttgag cttgaaaacg 6541 gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag ctggcactgc 6601 cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc aaagggtctc 6661 ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac cttgatgaga 6721 tcatcgagca aataagcgaa ttctccaaaa gagtgatcct cgccgacgct aacctcgata 6781 aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag gcagaaaaca 6841 ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag tacttcgaca 6901 ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc 6961 atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagacg 7021 gtggtagtgg aggttcagga ggatccgggg ggagcggagg gagcgctagc ggtggaggct 7081 ccggaggtgg atctaaaagg ccggcggcca cgaaaaaggc cggtcaggca aaaaagaaaa 7141 agggtggtag tggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg 7201 aggagaaccc tggacctgcg gccgcatgaa agggttcgat ccctaccggt tagtaatgag 7261 tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 7321 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg 7381 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 7441 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 7501 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc 7561 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 7621 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 7681 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 7741 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 7801 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 7861 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 7921 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 7981 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 8041 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 8101 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 8161 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 8221 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 8281 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 8341 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 8401 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 8461 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 8521 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 8581 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 8641 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 8701 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 8761 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 8821 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 8881 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 8941 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 9001 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 9061 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 9121 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 9181 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca 9241 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 9301 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 9361 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 9421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 9481 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 9541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 9601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 9661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 9721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 9781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 9841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 9901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 9961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 10021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 10081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 10141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 10201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 10261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 10321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 10381 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 10441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 10501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 10561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 10621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 10681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 10741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 10801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 10861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 10921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 10981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 11041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 11101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 11161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 11221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 11281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 11341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 11401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 11461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 11521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 11581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 11641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 11701 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc 11761 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct 11821 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg 11881 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg 11941 cgtt //