LOCUS       Exported               10272 bp ds-DNA     circular SYN 15-JUN-2021
DEFINITION  encodes CheRiff channelrhodopsin for lentiviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    HT041_FCMV_CheRiff_CFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   McNamara H, Salegame R, Al Tanoury Z, Xu H, Begum S, Ortiz G, 
            Pourquie O, Cohen A
  TITLE     Bioelectrical domain walls in homogeneous tissues
  JOURNAL   Nature Physics
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(72..932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     695..714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(933..1037)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1112..1131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1303..1682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1684..1882
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1900..2080
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2127..2252
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2745..2978
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3163..3207
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3505..3622
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3681..3984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3985..4188
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4185..4209
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4271..5218
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             5288..6004
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5330..5351)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5591..5610)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5938..5959
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6029..6617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6082..6102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6500..6511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6620..6636)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6621..6637)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7142..7322
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7348..7365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7354..7578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7624..8052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7711..7730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7921..7942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8061..8081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8066..8395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8246..8381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8308..8327
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8443..8490
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8509..8883
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9013..9134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9050..9069)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9104..9123
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9183..9199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9183..9199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9196..9218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9207..9223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9231..9261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9276..9297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9414..9431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9585..10173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9665..9684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
       61 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     1081 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1141 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     1201 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     1261 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     1321 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     1381 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1441 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1501 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1561 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1621 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1681 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     1741 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1801 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     1861 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
     1921 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     1981 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     2041 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     2101 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     2161 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     2221 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     2281 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     2341 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     2401 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     2461 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     2521 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     2581 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     2641 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     2701 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2761 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2821 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2881 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2941 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     3001 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     3061 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     3121 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     3181 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     3241 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     3301 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     3361 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     3421 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     3481 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3541 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3601 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     3661 aattaagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3781 gggactttcc attgacgtca atgggtggag catttacggt aaactgccca cttggcagta
     3841 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3901 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3961 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     4021 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4081 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     4141 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     4201 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     4261 tagaggatcc atgggcggag ctcctgctcc agacgctcac agcgccccac ctggaaacga
     4321 ttctgccgga ggcagtgagt accatgcccc agctggatat caagtgaatc caccctacca
     4381 ccccgtgcat gggtatgagg aacagtgcag ctccatctac atctactatg gggccctgtg
     4441 ggagcaggaa acagctaggg gcttccagtg gtttgccgtg ttcctgtctg ccctgtttct
     4501 ggctttctac ggctggcacg cctataaggc cagcgtggga tgggaggaag tgtacgtgtg
     4561 ctccgtggag ctgatcaaag tgattctgga gatctatttc gagttcacca gtcctgctat
     4621 gctgttcctg tacggaggga acattacccc atggctgaga tatgccgaat ggctgctgac
     4681 atgtcccgtg atcctgattc atctgtctaa catcaccggc ctgagtgagg catacaataa
     4741 gcggacaatg gctctgctgg tgtccgacct gggaactatt tgcatgggag tgacagccgc
     4801 tctggccact gggtgggtga agtggctgtt ttactgtatc ggcctggtgt atggaaccca
     4861 gacattctac aacgctggaa tcatctacgt ggagtcttac tatatcatgc ctgccggcgg
     4921 ctgtaagaaa ctggtgctgg ccatgactgc cgtgtactat tctagttggc tgatgtttcc
     4981 cggcctgttc atctttgggc ctgaaggcat gcacaccctg agcgtggctg ggtccactat
     5041 tggccatacc atcgccgacc tgctgtccaa gaatatttgg ggactgctgg ggcacttcct
     5101 gcggatcaaa attcacgagc atatcattat gtacggcgat atcaggagac cagtgagctc
     5161 ccagtttctg ggacgcaagg tggacgtgct ggccttcgtg acagaggaag ataaagtggc
     5221 ggccgccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat
     5281 caacgtggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5341 ggacggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac
     5401 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5461 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     5521 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgtaccat
     5581 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     5641 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     5701 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     5761 gaacggcatc aaggcccact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5821 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5881 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5941 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6001 gtaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     6061 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6421 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6601 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     6661 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6721 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6781 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6841 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6901 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     6961 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     7021 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     7081 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     7141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7321 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7381 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7441 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7501 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7561 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7621 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8041 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8221 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8401 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8461 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     8521 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     8581 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     8641 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     8701 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     8761 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     8821 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8881 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8941 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9001 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9061 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9121 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9241 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10261 aggatcttca cc
//
