LOCUS       Exported               10349 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  WT UPF1 inserted with GFP tagged on the N-terminus used to stably 
            integrate into cells.
ACCESSION   .
VERSION     .
KEYWORDS    pci-EGFP-UPF1-WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Fischer , J.W., Busa, V.F., Shao, S. Leung, A. K.L.
  TITLE     Structure-mediated RNA decay by UPF1 and G3BP1
  JOURNAL   Molecular Cell, Feb 3, 2020
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        538..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     692..712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          908..1040
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1023..1042
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1119..1128
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1125..1841
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1125..1841
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1170..1191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1431..1450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1778..1799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5245..5262)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    5280..5401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5291..5310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5345..5364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5587..6042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6159..6516
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6182..6202)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6367..6502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6429..6448
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6567..7361
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6621..6640)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7231..7250
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7425..7473
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7548..7567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7549..7567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7581..7597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7581..7597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7594..7616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7605..7621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7629..7659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7674..7695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7812..7829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7983..8571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8063..8082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8742..9602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9365..9384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9603..9707)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9734..10189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     10315..10337
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10329..10346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10330..10346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctgatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
       61 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
      121 atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      541 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
      601 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      661 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
      721 gaggtctata taagcagagc tcgtttagtg aaccgtcaga attgatctac catgagcttt
      781 attgcggtag tttatcacag ttaaattgct aacgcagtca gtgcttctga cacaacagtc
      841 tcgaacttaa gctgcagtga ctctcttaag gtagccttgc agaagttggt cgtgaggcac
      901 tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt
      961 gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca
     1021 ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag
     1081 tacttaatac gactcactat aggctagcgc taccggtcgc caccatggtg agcaagggcg
     1141 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1201 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1261 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1321 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1381 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1441 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1501 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1561 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1621 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1681 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1741 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1801 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcttagcgtg gaggcgtacg
     1861 ggcccagctc gcagactctc actttcctgg acacggagga ggccgagctg cttggcgccg
     1921 acacacaggg ctccgagttc gagttcaccg actttactct tcctagccag acgcagacgc
     1981 cccccggcgg ccccggcggc ccgggcggtg gcggcgcggg aggcccgggc ggcgcgggcg
     2041 cgggcgctgc ggcgggacag ctcgacgcgc aggttgggcc cgaaggcatc ctgcagaacg
     2101 gggctgtgga cgacagtgta gccaagacca gccagttgtt ggctgagttg aacttcgagg
     2161 aagatgaaga agacacctat tacacgaagg acctccccat acacgcctgc agttactgtg
     2221 gaatacacga tcctgcctgc gtggtttact gtaataccag caagaagtgg ttctgcaacg
     2281 gacgtggaaa tacttctggc agccacattg taaatcacct tgtgagggca aaatgcaaag
     2341 aggtgaccct gcacaaggac gggcccctgg gggagacagt cctggagtgc tacaactgcg
     2401 gctgtcgcaa cgtcttcctc ctcggcttca tcccggccaa agctgactca gtggtggtgc
     2461 tgctgtgcag gcagccctgt gccagccaga gcagcctcaa ggacatcaac tgggacagct
     2521 cgcagtggca gccgctgatc caggaccgct gcttcctgtc ctggctggtc aagatcccct
     2581 ccgagcagga gcagctgcgg gcacgccaga tcacggcaca gcagatcaac aagctggagg
     2641 agctgtggaa ggaaaaccct tctgccacgc tggaggacct ggagaagccg ggggtggacg
     2701 aggagccgca gcatgtcctc ctgcggtacg aggacgccta ccagtaccag aacatattcg
     2761 ggcccctggt caagctggag gccgactacg acaagaagct gaaggagtcc cagactcaag
     2821 ataacatcac tgtcaggtgg gacctgggcc ttaacaagaa gagaatcgcc tacttcactt
     2881 tgcccaagac tgactctgac atgcggctca tgcaggggga tgagatatgc ctgcggtaca
     2941 aaggggacct tgcgcccctg tggaaaggga tcggccacgt catcaaggtc cctgataatt
     3001 atggcgatga gatcgccatt gagctgcgga gcagcgtggg tgcacctgtg gaggtgactc
     3061 acaacttcca ggtggatttt gtgtggaagt cgacctcctt tgacaggatg cagagcgcat
     3121 tgaaaacgtt tgccgtggat gagacctcgg tgtctggcta catctaccac aagctgttgg
     3181 gccacgaggt ggaggacgta atcatcaagt gccagctgcc caagcgcttc acggcgcagg
     3241 gcctccccga cctcaaccac tcccaggttt atgccgtgaa gactgtgctg caaagaccac
     3301 tgagcctgat ccagggcccg ccaggcacgg ggaagacggt gacgtcggcc accatcgtct
     3361 accacctggc ccggcaaggc aacgggccgg tgctggtgtg tgctccgagc aacatcgccg
     3421 tggaccagct aacggagaag atccaccaga cggggctaaa ggtcgtgcgc ctctgcgcca
     3481 agagccgtga ggccatcgac tccccggtgt cttttctggc cctgcacaac cagatcagga
     3541 acatggacag catgcctgag ctgcagaagc tgcagcagct gaaagacgag actggggagc
     3601 tgtcgtctgc cgacgagaag cggtaccggg ccttgaagcg caccgcagag agagagctgc
     3661 tgatgaacgc agatgtcatc tgctgcacat gtgtgggcgc cggtgacccg aggctggcca
     3721 agatgcagtt ccgctccatt ttaatcgacg aaagcaccca ggccaccgag ccggagtgca
     3781 tggttcccgt ggtcctcggg gccaagcagc tgatccttgt aggcgaccac tgccagctgg
     3841 gcccagtggt gatgtgcaag aaggcggcca aggccgggct gtcacagtcg ctcttcgagc
     3901 gcctggtggt gctgggcatc cggcccatcc gcctgcaggt ccagtaccgg atgcaccctg
     3961 cactcagcgc cttcccatcc aacatcttct acgagggctc cctccagaat ggtgtcactg
     4021 cagcggatcg tgtgaagaag ggatttgact tccagtggcc ccaacccgat aaaccgatgt
     4081 tcttctacgt gacccagggc caagaggaga ttgccagctc gggcacctcc tacctgaaca
     4141 ggaccgaggc tgcgaacgtg gagaagatca ccacgaagtt gctgaaggca ggcgccaagc
     4201 cggaccagat tggcatcatc acgccctacg agggccagcg ctcctacctg gtgcagtaca
     4261 tgcagttcag cggctccctg cacaccaagc tctaccagga ggtggagatc gccagtgtgg
     4321 acgcctttca gggacgcgag aaggacttca tcatcctgtc ctgtgtgcgg gccaacgagc
     4381 accaaggcat tggcttttta aatgacccca ggcgtctgaa cgtggccctg accagagcaa
     4441 ggtatggcgt catcattgtg ggcaacccga aggcactatc aaagcagccg ctctggaacc
     4501 acctgctgaa ctactataag gagcagaagg tgctggtgga ggggccgctc aacaacctgc
     4561 gtgagagcct catgcagttc agcaagccac ggaagctggt caacactatc aacccgggag
     4621 cccgcttcat gaccacagcc atgtatgatg cccgggaggc catcatccca ggctccgtct
     4681 atgatcggag cagccagggc cggccttcca gcatgtactt ccagacccat gaccagattg
     4741 gcatgatcag tgccggccct agccacgtgg ctgccatgaa cattcccatc cccttcaacc
     4801 tggtcatgcc acccatgcca ccgcctggct attttggaca agccaacggg cctgctgcag
     4861 ggcgaggcac cccgaaaggc aagactggtc gtgggggacg ccagaagaac cgctttgggc
     4921 ttcctggacc cagccagact aacctcccca acagccaagc cagccaggat gtggcgtcac
     4981 agcccttctc tcagggcgcc ctgacgcagg gctacatctc catgagccag ccttcccaga
     5041 tgagccagcc cggcctctcc cagccggagc tgtcccagga cagttacctt ggtgacgagt
     5101 ttaaatcaca aatcgacgtg gcgctctcac aggactccac gtaccaggga gagcgggctt
     5161 accagcatgg cggggtgacg gggctgtccc agtattaaaa ggtggcggcg gaagagctaa
     5221 agagtcgacc cgggcggccg cttcccttta gtgagggtta atgcttcgag cagacatgat
     5281 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     5341 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5401 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     5461 ttaaagcaag taaaacctct acaaatgtgg taaaatccga taaggatcga tccgggctgg
     5521 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5581 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5641 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5701 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5761 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5821 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5881 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5941 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6001 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     6061 ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct
     6121 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg
     6181 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6241 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6301 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6361 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6421 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6481 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca
     6541 gtctcgaact taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc
     6601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6721 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7081 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7381 aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat
     7441 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atcccctagg
     7501 cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc tatagtgagt
     7561 cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7741 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7801 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8401 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8881 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9721 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9781 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9841 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9901 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9961 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10021 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10081 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10141 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat
    10201 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10261 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10321 cacgacgttg taaaacgacg gccagtgcc
//
