LOCUS Exported 10027 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2. ACCESSION . VERSION . KEYWORDS pAY19 (OsTIR1(F74A) mAID-EGFP-NLS) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10027) AUTHORS Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT. TITLE The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. JOURNAL Nat Commun (2020) 11(1):5701 REFERENCE 2 (bases 1 to 10027) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10027 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(7..462) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(144..165) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 356..375 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 589..611 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 603..620 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 604..620 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 630..649 /label=T7 /note="T7 promoter, forward primer" promoter 630..648 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 884..917 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(1474..2073) /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 2054..2073 /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" promoter complement(2113..2612) /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(2161..2180) /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind 2554..2577 /label=MSCV-rev /note="Murine stem cell virus, reverse primer" protein_bind 2673..2706 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 2733..2854 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2744..2763) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2798..2817 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(3005..3025) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(3062..3778) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(3062..3778) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3104..3125) /label=EGFP-C /note="EGFP, forward primer" primer_bind 3453..3472 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3712..3733 /label=EGFP-N /note="EGFP, reverse primer" CDS complement(3785..3988) /codon_start=1 /product="minimal degron from the auxin-responsive IAA17 protein of Arabidopsis thaliana (Kubota et al., 2013)" /label=mini-AID /note="acts as an auxin-inducible degron (AID) in the presence of TIR1" /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA AAFVKVSMDGAPYLRKIDLRMYK" CDS complement(3995..4051) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(5861..5880) /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" intron 5888..6905 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind complement(5942..5964) /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" promoter complement(6907..7184) /label=chicken beta-actin promoter enhancer 7186..7565 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind complement(7843..7863) /label=T3 /note="T3 promoter, forward primer" promoter complement(7843..7861) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7882..7898) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7882..7898) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7895..7917) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7906..7922 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7930..7960) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7975..7996 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8113..8130) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8284..8872) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8364..8383) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9043..9903) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9666..9685 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9904..10008) /gene="bla" /label=AmpR promoter ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc 481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta 661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag 721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta 781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa 841 gtaaaagcaa gaaagatctt agtgaacctc ttcgagggac ctaataactt cgtatagcat 901 acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat ggaattagaa 961 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct 1021 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc 1081 aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa 1141 tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt 1201 atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc 1261 acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct 1321 tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag 1381 atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc gtgcgctcct 1441 ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca 1501 ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc 1561 gtagaagggg aggttgtggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc 1621 ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg 1681 cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag 1741 gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc 1801 gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc 1861 ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag 1921 ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc 1981 ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt 2041 ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttcagctgct cgagatctag 2101 atggatgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg 2161 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct 2221 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc 2281 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc 2341 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca 2401 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc 2461 gaaggggcca ccaaagaacg gagtcggttg gcgcctaccg gtggatgtgg aatgtgtgcg 2521 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc 2581 agactgcctt gggaaaagcg cctcccctac ccggtagaat ttcgaggtcg agatcctaag 2641 cttggctgga cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga 2701 agttatatta agggttattg aatatgatcg cttaagatac attgatgagt ttggacaaac 2761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 2821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat 2881 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg 2941 tggtatggct gattatgatc agttatctag atccggtgga tcccgggccc gcggtaccgt 3001 cgatgacctt tcttttcttc ttggggtcga ctgcagaatt cgaagcttgg gcgcctgcac 3061 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga 3121 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt 3181 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg 3241 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt 3301 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt 3361 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca 3421 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga 3481 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct 3541 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg 3601 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt 3661 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt 3721 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgc 3781 tagctttata catcctcaaa tcgattttcc tcaagtacgg tgctccgtcc attgatacct 3841 tcacgaacgc cgccgcctcc gggccaccgc ttgatttttg gcaggaaacc atcacgttct 3901 tccggtatga tctcaccggt ggccatccca caacttgtgc cttggccgga ggtttggctg 3961 gatctttagg acaagcactc ttctccttag atctaggtcc agggttctcc tccacgtctc 4021 cagcctgctt cagcaggctg aagttagtag ctccgcttcc tgatctcaga atcttcacaa 4081 agttgggagc atcatctctt gcccctgcgg tggtccgata cacatacagt ttctccacct 4141 ttggcaggtc gccatgattt tcctccattt cgttagaccc gtcccgctca ttaatgactt 4201 ccacgttcag cataggcatt ttactagcca ggacctgaca gcccttcaga gtcacattgc 4261 aactagacat ccacaggctc ctcattgtct cgtatctggc aaagtttccc agcagagcgg 4321 catccccgaa agggctgtcc cggatttcca gttttcgcag attcttacac ccgttcatca 4381 cgtgcatcat gcccttatcg gagtctccgg caaatgcaat gctcagcatc tccagctgct 4441 tagcatactt gccgatgtac atgaacactt tgtctgtcag caggccggaa atgctcagcc 4501 tctggagtcc cttgcactct ctcacgatag ctccaaaccc ttcatccagt ggctgggatg 4561 tgaccacgtc gggtttgcct ggttccagaa tacacagccg aaatcgggtg aaattagggc 4621 agttcttggc gacggtcacc agtgcagcat tagtcatctg atgacagaaa tacagcaggc 4681 tgttcagctt tgggcatccc agtgagactg ccaccaggcc ttcctcagtg acagcactat 4741 atccggccac gtaaaaatca gatgggaaca ctctcagctc ctggaggtcc ttgcaggagc 4801 tggcgaccac ctggagcccc ttatcggaaa tacagtccag cacccacagt cgctggagtt 4861 tcacgcagcg tgagatcatc tttgtcagat cactggcgtc cagggttggt gcatagctca 4921 ggttcaggcc tgtcagctgt gcacacagag ggtagataaa gctcaggcac acgggtgaag 4981 catcccagaa tcctgacaga ctcctcagca ttttacactt ctccagggca gaagtcagtt 5041 taaagtagga ttctgtctgg aaatcgtctg tcagattgcc ggttcccaga tcctccaggt 5101 taggggttct cagcagaatc ttagccagag tgtccacgct cacgctccgg ttcagccgca 5161 gagacctcag gttggggctt cttgagacca gccgctccag ggacccagca ttcacttcgc 5221 ctttgatgca ggcaaagttc agggacacca ggctggtaca tgagtctggg aagcaagaca 5281 gccatctggg gcctctatcc tccacttcat tctcctggag gtccagctcc cgcagcagct 5341 tacagtggga ggccacggct gccagtccat cggtgctgaa gccttcgcaa gaaatcagca 5401 ccagtgcgcg aaacctaggg aagctccgag ccagcagctc cagactttcg tcagagacca 5461 ccattcgctt catgcgcagt tcctccagtc catgacagcc gcgagcggct gcctcgatcc 5521 aagggccggc gtatcctccc caatcagggg gcaccaggtt aaagtcggcg gcgtggggtt 5581 tccccttgac ggtcagtgcg cgcacatttg gaaacctagc ggcgaccctg cctgctctca 5641 cagcatagca gttgccgacg aacactcctc tccggctcag gcgttcgatc tcgtaccaca 5701 ctttgcagac caggctcact gtgtttctat ctctctgtgc aggcaggaag ctaaaaatgt 5761 gttcgacgac ctcttcagga aagtatgtca tggtggcagc gctatctaga tgcattcgcg 5821 aggtaccgag ctcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc 5881 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc 5941 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc 6001 cccgcggaca gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa 6061 ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag 6121 agggggtgcg gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc 6181 cctgcgccct ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac 6241 agccgccggc gctccggggg ccgccgcgcc cctcccccga gccctccccg gcccgaggcg 6301 gccccgcccc gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg 6361 cgccacgccc cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag 6421 gggggtgcag ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca 6481 cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac 6541 cgcccccggc cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc 6601 cgcagcgctc acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac 6661 acacgcacgc accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa 6721 ggctttcacg cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc 6781 ccggaggaga agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc 6841 ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcag cgtcgcagcg 6901 actccccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat 6961 aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc 7021 cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc ccccccccgc 7081 ccccatcgct gcacaaaata attaaaaaat aaataaatac aaaattgggg gtggggaggg 7141 gggggagatg gggagagtga agcagaacgt ggggctcacc tcgaccatgg taatagcgat 7201 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata 7261 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata 7321 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg 7381 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg 7441 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat 7501 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca 7561 atgtcgagca tgcatctaga gggcccaagt gatctccaaa aaataagtac tttttgactg 7621 taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa 7681 agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact 7741 taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct 7801 accttcagca agcccagcag atccgagctc cagcttttgt tccctttagt gagggttaat 7861 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7921 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7981 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 8041 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 8101 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8701 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 9001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 9061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 10021 aaaagtg //