LOCUS       Exported               10952 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  OsTIR1(F74G)-P2A-mAID-EGFP-Nluc.
ACCESSION   .
VERSION     .
KEYWORDS    pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT.
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun (2020) 11(1):5701
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    33..54
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        69..99
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     112..134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     131..147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     131..147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     166..186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        168..186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        464..843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        845..1122
                     /label=chicken beta-actin promoter
     intron          1124..2141
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2065..2087
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2149..2168
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3978..4034
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4041..4244
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             4251..4967
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4251..4967
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4296..4317)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4557..4576)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4904..4925
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5004..5519
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     polyA_signal    5649..5770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5686..5705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5740..5759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5801..5834)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        5895..6394
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5930..5953)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6327..6346
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6455..7474
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    complement(8037..8070)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(8305..8324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8306..8324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8334..8351)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8334..8350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8343..8365)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8492..8947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8579..8598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8789..8810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8973..9077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9078..9938
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9296..9315)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10109..10697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10598..10617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10851..10868
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
       61 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
      181 taaagggaac aaaagctgga gctcggatct gctgggcttg ctgaaggtag ggggtcaaga
      241 accagaggtg taaagtactt gagtaatttt acttgattac tgtacttaag tattattttt
      301 ggggattttt actttacttg agtacaatta aaaatcaata cttttacttt tacttaatta
      361 cattttttta gaaaaaaaag tactttttac tccttacaat tttatttaca gtcaaaaagt
      421 acttattttt tggagatcac ttgggccctc tagatgcatg ctcgacattg attattgact
      481 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      601 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      661 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      781 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      841 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      901 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      961 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
     1021 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     1081 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct
     1141 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     1201 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     1261 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     1321 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     1381 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     1441 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1501 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1561 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1621 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1681 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     1741 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1801 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1861 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1921 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1981 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     2041 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2101 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2161 ttattgtgct gtctcatcat tttggcaaag aattcgagct cggtacctcg cgaatgcatc
     2221 tagatagcgc tgccaccatg acatactttc ctgaagaggt cgtcgaacac atttttagct
     2281 tcctgcctgc acagagagat agaaacacag tgagcctggt ctgcaaagtg tggtacgaga
     2341 tcgaacgcct gagccggaga ggagtgttcg tcggcaactg ctatgctgtg agagcaggca
     2401 gggtcgccgc taggtttcca aatgtgcgcg cactgaccgt caaggggaaa ccccacggcg
     2461 ccgactttaa cctggtgccc cctgattggg gaggatacgc cggcccttgg atcgaggcag
     2521 ccgctcgcgg ctgtcatgga ctggaggaac tgcgcatgaa gcgaatggtg gtctctgacg
     2581 aaagtctgga gctgctggct cggagcttcc ctaggtttcg cgcactggtg ctgatttctt
     2641 gcgaaggctt cagcaccgat ggactggcag ccgtggcctc ccactgtaag ctgctgcggg
     2701 agctggacct ccaggagaat gaagtggagg atagaggccc cagatggctg tcttgcttcc
     2761 cagactcatg taccagcctg gtgtccctga actttgcctg catcaaaggc gaagtgaatg
     2821 ctgggtccct ggagcggctg gtctcaagaa gccccaacct gaggtctctg cggctgaacc
     2881 ggagcgtgag cgtggacact ctggctaaga ttctgctgag aacccctaac ctggaggatc
     2941 tgggaaccgg caatctgaca gacgatttcc agacagaatc ctactttaaa ctgacttctg
     3001 ccctggagaa gtgtaaaatg ctgaggagtc tgtcaggatt ctgggatgct tcacccgtgt
     3061 gcctgagctt tatctaccct ctgtgtgcac agctgacagg cctgaacctg agctatgcac
     3121 caaccctgga cgccagtgat ctgacaaaga tgatctcacg ctgcgtgaaa ctccagcgac
     3181 tgtgggtgct ggactgtatt tccgataagg ggctccaggt ggtcgccagc tcctgcaagg
     3241 acctccagga gctgagagtg ttcccatctg atttttacgt ggccggatat agtgctgtca
     3301 ctgaggaagg cctggtggca gtctcactgg gatgcccaaa gctgaacagc ctgctgtatt
     3361 tctgtcatca gatgactaat gctgcactgg tgaccgtcgc caagaactgc cctaatttca
     3421 cccgatttcg gctgtgtatt ctggaaccag gcaaacccga cgtggtcaca tcccagccac
     3481 tggatgaagg gtttggagct atcgtgagag agtgcaaggg actccagagg ctgagcattt
     3541 ccggcctgct gacagacaaa gtgttcatgt acatcggcaa gtatgctaag cagctggaga
     3601 tgctgagcat tgcatttgcc ggagactccg ataagggcat gatgcacgtg atgaacgggt
     3661 gtaagaatct gcgaaaactg gaaatccggg acagcccttt cggggatgcc gctctgctgg
     3721 gaaactttgc cagatacgag acaatgagga gcctgtggat gtctagttgc aatgtgactc
     3781 tgaagggctg tcaggtcctg gctagtaaaa tgcctatgct gaacgtggaa gtcattaatg
     3841 agcgggacgg gtctaacgaa atggaggaaa atcatggcga cctgccaaag gtggagaaac
     3901 tgtatgtgta tcggaccacc gcaggggcaa gagatgatgc tcccaacttt gtgaagattc
     3961 tgagatcagg aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg
     4021 agaaccctgg acctagatct aaggagaaga gtgcttgtcc taaagatcca gccaaacctc
     4081 cggccaaggc acaagttgtg ggatggccac cggtgagatc ataccggaag aacgtgatgg
     4141 tttcctgcca aaaatcaagc ggtggcccgg aggcggcggc gttcgtgaag gtatcaatgg
     4201 acggagcacc gtacttgagg aaaatcgatt tgaggatgta taaagctagc atggtgagca
     4261 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4321 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4381 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4441 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4501 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4561 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4621 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4681 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4741 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4801 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4861 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4921 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt gcaggcgccc
     4981 aagcttcgaa ttctgcagtc gacatggtct tcacactcga agatttcgtt ggggactggc
     5041 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     5101 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     5161 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     5221 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     5281 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     5341 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     5401 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     5461 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag
     5521 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     5581 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5641 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5761 atgtatctta acgcgtcgat catattcaat aacccttaat ataacttcgt ataatgtatg
     5821 ctatacgaag ttattaggtc tgaagaggag tttacgtcca gccaagctta ggatctcgac
     5881 ctcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     5941 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     6001 atccaccggt aggcgccaac cgactccgtt ctttggtggc cccttcgcgc caccttctac
     6061 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     6121 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     6181 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     6241 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     6301 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     6361 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag atctcgagca
     6421 gctgaagctt accgctagca tggatagatc cggaaagcct gaactcaccg cgacgtctgt
     6481 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     6541 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     6601 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     6661 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     6721 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     6781 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     6841 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     6901 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     6961 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     7021 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     7081 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     7141 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     7201 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     7261 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     7321 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     7381 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     7441 acgccccagc actcgtccga gggcaaagga ataggctagc cgcccgcccc acgacccgca
     7501 gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg atatcagatc cccgggatgc
     7561 agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca
     7621 tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta
     7681 cgggggtggg ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact
     7741 attgctttat gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt
     7801 aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca
     7861 ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag
     7921 tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta
     7981 ttgttttgcc aagttctaat tccatcagaa gctggtcgag atccggaacc cttaatataa
     8041 cttcgtataa tgtatgctat acgaagttat taggtccctc gaagaggttc actaagatct
     8101 ttcttgcttt tacttttact tccttaatac tcaagtacaa ttttaatgga gtactttttt
     8161 acttttactc aagtaagatt ctagccagat acttttactt ttaattgagt aaaattttcc
     8221 ctaagtactt gtactttcac ttgagtaaaa tttttgagta ctttttacac ctctgtcaag
     8281 aaccatatgc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8341 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8401 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8461 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8941 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9001 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9061 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9121 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9181 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9241 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9301 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9361 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9421 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9481 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9541 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9601 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9661 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9721 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9781 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9841 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9901 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9961 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10021 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    10261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10801 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10861 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10921 ttcattaatg cagctggcac gacaggtttc cc
//
