LOCUS Exported 5188 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION P3f. ACCESSION . VERSION . KEYWORDS pBT138b-proB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5188) AUTHORS Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR TITLE Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity JOURNAL Nat Biotechnol (2020) REFERENCE 2 (bases 1 to 5188) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5188 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(66..857) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" promoter complement(858..929) /gene="bla" /label=AmpR promoter rep_origin 957..1545 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1446..1465 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1699..1716 /label=L4440 /note="L4440 vector, forward primer" misc_feature 1731..1871 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(1797..1816) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1957..1979 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(1973..2164) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" ORIGIN 1 actcctcagc aaatataatg accctcttga taacccaaga gggcattttt taatgcccat 61 ggcgtttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc 121 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct 181 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca 241 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact 301 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt 361 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct 421 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc 481 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt 541 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct 601 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc 661 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca 721 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc 781 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg 841 atcaccgctt ccctcatact cttccttttt caatattatt gaagcattta tcagggttat 901 tgtctcatga gcgtatacat atttgaatgt atttagaaaa ataggccaaa tagggtttga 961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1021 gtggtttgtt tgccggatca agagctacca cctctttttc cgaaggtaac tggcttcagc 1081 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 1621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 1681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggatgagcg cctgatgcgg 1741 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca 1801 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg 1861 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 1921 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 1981 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt 2041 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa 2101 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg 2161 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac 2221 gagagaggat gctcacgata cgggttactg atgatgaaca agaggacatc cggtcaaata 2281 aaacgaaagg ctcagtcgaa agactgggcc tttcgttttg ctgaggagac ttagggactc 2341 tcacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat 2401 aactttacgg gcatgcataa ggctcgtaat atatattcag ggagaccaca acggtttccc 2461 tctacaaata attttgttta acttttacta gagtgggacc ctacctgcag gtgcagtact 2521 acatcatcag gcatgaacac gattaacatc gctaagaacg acttctctga catcgaactg 2581 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag 2641 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag 2701 tgataactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc 2761 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc 2821 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac 2881 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct 2941 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac 3001 cttaaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac 3061 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc 3121 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc 3181 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta 3241 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc 3301 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag 3361 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg 3421 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg 3481 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg 3541 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag 3601 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg 3661 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc 3721 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc 3781 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt 3841 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt 3901 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct 3961 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca 4021 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt 4081 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt 4141 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt 4201 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag 4261 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc 4321 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg 4381 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg 4441 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag 4501 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac 4561 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg 4621 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga 4681 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg 4741 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc 4801 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct 4861 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg 4921 tgggcacacg agaagtacgg tatcgaatct tttgcactga ttcacgactc cttcggtacc 4981 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat 5041 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct 5101 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta 5161 gagtccgact tcgcgttcgc ctaatggc //