LOCUS       Exported               10282 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian ACBEmax expression vector (pSI-632).
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-ACBEmax
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto
            S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, 
            Nureki O, Tomita M, Aburatani H, Yachie N
  TITLE     Base editors for simultaneous introduction of C-to-T and A-to-G 
            mutations.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 
            10.1038/s41587-020-0509-0.
  PUBMED    32483365
REFERENCE   2  (bases 1 to 10282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             712..732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1921..6021
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6229..6294
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6301..6984
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             6985..7233
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7264..7512
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7555..7575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7604..7621)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7610..7834
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7905..7921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7905..7921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7918..7940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7929..7945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7953..7983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7998..8019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8136..8153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8307..8895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8387..8406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9066..9926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9689..9708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9927..10031)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10110..10129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctggtttagt gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata
      661 gggagagccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
      721 aagcggaaag tctctgaagt cgagtttagc cacgagtatt ggatgaggca cgcactgacc
      781 ctggcaaagc gagcatggga tgaaagagaa gtccccgtgg gcgccgtgct ggtgcacaac
      841 aatagagtga tcggagaggg atggaacagg ccaatcggcc gccacgaccc taccgcacac
      901 gcagagatca tggcactgag gcagggaggc ctggtcatgc agaattaccg cctgatcgat
      961 gccaccctgt atgtgacact ggagccatgc gtgatgtgcg caggagcaat gatccacagc
     1021 aggatcggaa gagtggtgtt cggagcacgg gacgccaaga ccggcgcagc aggctccctg
     1081 atggatgtgc tgcaccaccc cggcatgaac caccgggtgg agatcacaga gggaatcctg
     1141 gcagacgagt gcgccgccct gctgagcgat ttctttagaa tgcggagaca ggagatcaag
     1201 gcccagaaga aggcacagag ctccaccgac tctggaggat ctagcggagg atcctctgga
     1261 agcgagacac caggcacaag cgagtccgcc acaccagaga gctccggcgg ctcctccgga
     1321 ggatcctctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc
     1381 aagagggcac gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga
     1441 gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa
     1501 attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc
     1561 ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc
     1621 ggccgcgtgg tgtttggcgt gaggaacgca aaaaccggcg ccgcaggctc cctgatggac
     1681 gtgctgcact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat
     1741 gaatgtgccg ccctgctgtg ctatttcttt cggatgccta gacaggtgtt caatgctcag
     1801 aagaaggccc agagctccac cgactccgga ggatctagcg gaggctcctc tggctctgag
     1861 acacctggca caagcgagag cgcaacacct gaaagcagcg ggggcagcag cggggggtca
     1921 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     1981 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     2041 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     2101 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     2161 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     2221 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     2281 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     2341 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     2401 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     2461 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     2521 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     2581 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     2641 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     2701 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     2761 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     2821 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     2881 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     2941 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     3001 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     3061 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     3121 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     3181 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     3241 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     3301 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     3361 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     3421 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     3481 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     3541 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     3601 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     3661 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     3721 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     3781 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     3841 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     3901 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     3961 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     4021 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     4081 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     4141 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     4201 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     4261 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     4321 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     4381 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     4441 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     4501 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     4561 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     4621 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     4681 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     4741 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     4801 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     4861 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     4921 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     4981 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     5041 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     5101 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     5161 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     5221 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     5281 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     5341 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     5401 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     5461 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     5521 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     5581 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     5641 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     5701 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     5761 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     5821 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     5881 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     5941 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     6001 ctgtctcagc tgggaggtga cggcggagga ggaactggag gaggaggatc agcagaatat
     6061 gtgagagcac tgtttgactt taatgggaat gacgaagaag acctgccctt caagaagggc
     6121 gacatcctgc ggatcagaga taagcctgag gagcagtggt ggaacgccga ggactctgag
     6181 ggcaagagag gcatgatcct ggtgccctac gtggagaagt acagcggcga ttataaggac
     6241 cacgatggcg actacaagga ccacgacatc gactacaagg atgacgatga caagtctggc
     6301 tcctcagaga ctgggcctgt cgccgtcgat ccaaccctgc gccgccggat tgaacctcac
     6361 gagtttgaag tgttctttga cccccgggag ctgagaaagg agacatgcct gctgtacgag
     6421 atcaactggg gaggcaggca ctccatctgg aggcacacct ctcagaacac aaataagcac
     6481 gtggaggtga acttcatcga gaagtttacc acagagcggt acttctgccc caataccaga
     6541 tgtagcatca catggtttct gagctggtcc ccttgcggag agtgtagcag ggccatcacc
     6601 gagttcctgt ccagatatcc acacgtgaca ctgtttatct acatcgccag gctgtatcac
     6661 cacgcagacc caaggaatag gcagggcctg cgcgatctga tcagctccgg cgtgaccatc
     6721 cagatcatga cagagcagga gtccggctac tgctggcgga acttcgtgaa ttattctcct
     6781 agcaacgagg cccactggcc taggtaccca cacctgtggg tgcgcctgta cgtgctggag
     6841 ctgtattgca tcatcctggg cctgccccct tgtctgaata tcctgcggag aaagcagccc
     6901 cagctgacct tctttacaat cgccctgcag tcttgtcact atcagaggct gccaccccac
     6961 atcctgtggg ccacaggcct gaagactaat ctgagcgaca tcattgagaa ggagactggg
     7021 aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga ggaagtgatc
     7081 ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc cacagatgag
     7141 aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct ggtcatccag
     7201 gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggaga atctggaggc
     7261 agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt catccaggag
     7321 agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc tgagagcgat
     7381 atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct gctgacatcc
     7441 gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg agagaacaaa
     7501 atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag
     7561 aagaagagga aagtctaata atgagtttaa acccgctgat cagcctcgac tgtgccttct
     7621 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7681 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7741 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7801 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7861 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7921 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7981 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8041 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8101 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8161 gtcgttctgc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8221 gaatcatggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8761 atctgcgctc tgctgaagcc agttaccttc tgaaaaagag ttggtagctc ttgatccggc
     8821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac
     8941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9361 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9661 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
    10081 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
    10141 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    10201 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    10261 tttgcgctgc ttcgcgatgt ac
//
