LOCUS Exported 4524 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Template to synthesize riboprobes for in situ hybridization (ISH) for Ncam2. ACCESSION . VERSION . KEYWORDS pISH-OCAM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4524) AUTHORS Zapiec B, Mombaerts P TITLE The Zonal Organization of Odorant Receptor Gene Choice in the Main Olfactory Epithelium of the Mouse. JOURNAL Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 10.1016/j.celrep.2020.02.110. PUBMED 32209480 REFERENCE 2 (bases 1 to 4524) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4524 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 73..95 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 87..104 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 88..104 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 111..130 /label=T7 /note="T7 promoter, forward primer" promoter 111..129 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 160..169 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 164..173 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(1776..1794) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1777..1794) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(1812..1828) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1812..1828) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1825..1847) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1836..1852 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1860..1890) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1905..1926 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2043..2060) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2214..2802) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2294..2313) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2973..3833) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3596..3615 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3834..3938) /gene="bla" /label=AmpR promoter rep_origin complement(4016..4471) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4153..4174) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4365..4384 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 61 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact 121 cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga 181 attcgattga gaagtggtgt cccctcaaga gttcaagcaa ggggaggatg cagaagtggt 241 ttgcagagtg agcagttccc cagcccctgc ggtcagctgg ttgtaccaca atgaggaagt 301 caccaccatc cccgacaatc ggtttgcagt gcttgcaaac aataatttgc agatcctcaa 361 tatcaataaa agtgatgaag gtatatacag atgtgaagga agagtggagg cgaggggaga 421 gattgacttc cgggatatca ttgttattgt taacgttcca ccagcaatca tgatgcccca 481 gaagtccttc aatgctacag cagagagagg agaagagatg accttaacat gcaaggcctc 541 gggctcccca gatccaacca tctcttggtt caggaacggc aaactcattg aagaaaatga 601 aaagtatatt ttaaagggca gtaatacaga gctcactgtc aggaacataa tcaataaaga 661 cgggggctct tatgtctgca aagcaacaaa caaggcagga gaagatcaaa agcaggcctt 721 ccttcaagtc tttgtacagc ctcatatatt acaacttaaa aatgagacaa catctgagaa 781 tggtcatgtc acactcgtat gtgaagcaga aggggagcct gttccagaaa tcacatggaa 841 aagagccata gatggagtca tgttttctga aggtgataag agtccggatg gccgcatcga 901 agttaaaggg cagcatggac gctcttcact gcacattaga gatgtgaagt tgtcagattc 961 ggggagatat gactgtgagg ccgctagtcg gattggtggg caccagagga gcatgcacct 1021 ggacatcgaa tatgctccta agtttgtttc aaatcagaca atgtattact cttgggaagg 1081 aaatccaatc aacataagtt gtgatgtgac agcaaaccca cctgcgtcaa tccactggag 1141 aagagagaaa ctgctcttac cagctaagaa tacgactcat ttaaaaactc acagtgtagg 1201 aagaaagatg atactcgaga ttgcccccac atcagacaat gactttggac gatataactg 1261 cacagctact aaccgtatag gcacaagatt tcaggaatat attcttgaat tagcagatgt 1321 cccctctagt ccccatggag tgaagattat agagctgtca cagaccacag ccaagatctc 1381 tttcaataaa cccgaatccc atggaggtgt gcctattcat cactatcaag tggatgtcaa 1441 agaagtggca tcagaaacct ggaaaatagt acgctcccat ggagttcaaa cgatggttgt 1501 tttgagcagt ctggaaccaa atacgactta cgaaattagg gttgcagcag tgaatggcaa 1561 aggtcaagga gactacagta aaatagaaat attccagaca ctgccagtcc gtgagccaag 1621 tcccccttcc atacatggac agccaagcag tggcaagagt tttaaaatca gcatcaccaa 1681 gcaagatgat ggaggaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga 1741 gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg 1801 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 1861 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 1921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 1981 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 2041 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2101 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2161 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2221 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2281 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2341 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2401 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2461 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2521 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2581 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 2641 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 2701 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 2761 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 2821 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 2881 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 2941 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 3001 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 3061 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 3121 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 3181 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 3241 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 3301 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 3361 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 3421 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 3481 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 3541 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 3601 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 3661 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 3721 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 3781 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 3841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3961 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt 4021 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 4081 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 4141 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 4201 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 4261 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 4321 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 4381 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 4441 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt 4501 gggaagggcg atcggtgcgg gcct //