LOCUS Exported 10256 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses EGFP-tagged recycling endosome marker. ACCESSION . VERSION . KEYWORDS pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10256) AUTHORS Schormann W, Hariharan S, Andrews DW TITLE A reference library for assigning protein subcellular localizations by image-based machine learning. JOURNAL J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090. PUBMED 31968357 REFERENCE 2 (bases 1 to 10256) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10256 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 73..706 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 753..878 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1375..1608 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1793..1837 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2100..2215 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2410..3591 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2640..3582 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3539..3559 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 3635..3644 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3641..4357 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3641..4357 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3686..3707) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3947..3966) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4294..4315 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5078..5651 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5245..5262) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5472..5491 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5684..6283 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5684..5703) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6180..6200 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6297..6885 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6350..6370) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6768..6779) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7092..7725 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7853..7869) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7853..7869) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7866..7888) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7877..7893 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7901..7931) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7946..7967 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8084..8101) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8255..8843) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8335..8354) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9014..9874) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9637..9656 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9875..9979) /gene="bla" /label=AmpR promoter polyA_signal 10027..10161 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10064..10083) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10118..10137 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac 61 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt 121 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 181 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 241 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 301 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 361 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 421 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 481 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 721 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 781 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 841 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 901 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 961 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 1021 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 1081 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 1141 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 1201 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga 1261 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1321 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1381 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 1441 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1501 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1561 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1621 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1681 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 1741 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1801 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1861 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1921 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1981 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 2041 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct 2101 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 2161 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg 2221 tttattacag ggacagcaga gatccagttt atcgatgagt aattcataca aaaggactcg 2281 cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta gaacccagag 2341 atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt ggagaagagc 2401 atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact 2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg 2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc 2701 cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt 2761 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga 2821 gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc 2881 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg 2941 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg 3001 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg 3061 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct 3121 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc 3181 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc 3241 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa 3301 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc 3361 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg 3421 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg 3481 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc 3541 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggatctatt 3601 tccggtgaat tcctcgagac tagcgctacc ggtcgccacc atggtgagca agggcgagga 3661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 3721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 3781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 3841 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 3901 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 3961 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 4021 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 4081 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 4141 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 4201 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 4261 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 4321 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagccat 4381 ggggacccgg gacgacgagt acgactacct attcaaagtg gtgctcatcg gggactcagg 4441 cgtgggcaag agcaacctgc tgtcgcgctt cacccgcaac gagttcaacc tggagagcaa 4501 gagcaccatc ggcgtggagt tcgccacccg cagcatccag gtggacggca agaccatcaa 4561 ggcgcagatc tgggacaccg ctggccagga gcgctaccgc gccatcacct ccgcgtacta 4621 ccgtggtgca gtgggcgccc tgctggtgta cgacatcgcc aagcacctga cctatgagaa 4681 cgtggagcgc tggctgaagg agctgcggga ccacgcagac agcaacatcg tcatcatgct 4741 ggtgggcaac aagagtgacc tgcgccacct gcgggctgtg cccactgacg aggcccgcgc 4801 cttcgcagaa aagaacaact tgtccttcat cgagacctca gccttggatt ccactaacgt 4861 agaggaagca ttcaagaaca tcctcacaga gatctaccgc atcgtgtcac agaaacagat 4921 cgcagaccgc gctgcccacg acgagtcccc ggggaacaac gtggtggaca tcagcgtgcc 4981 gcccaccacg gacggacaga agcccaacaa gctgcagtgc tgccagaacc tgtgataaga 5041 attctgcagt cgacggtacc gcgggcccgg gatcccgccc ctctccctcc ccccccccta 5101 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 5161 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 5221 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 5281 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 5341 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 5401 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 5461 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 5521 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 5581 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 5641 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc 5701 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc 5761 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt 5821 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 5881 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 5941 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 6001 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 6061 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 6121 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 6181 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 6241 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc tggaacaatc 6301 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 6361 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 6421 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 6481 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 6541 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 6601 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 6661 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 6721 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6781 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 6841 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg 6901 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 6961 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca 7021 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7081 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg 7141 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt 7201 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt 7261 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat 7321 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca 7381 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa 7441 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg 7501 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag 7561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 7621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 7681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 7741 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt 7801 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 7861 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 7921 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 7981 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 8041 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 8101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 8161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 8221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 8281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 8341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 8401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 8461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 8521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 8581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 8641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 8701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 8761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 8821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 8881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 8941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 9001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 9061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 9121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 9181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 9241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 9301 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 9361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 9421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 9481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 9541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 9601 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 9661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 9721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 9781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 9841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 9901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 9961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 10021 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10141 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 10201 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaac //