LOCUS Exported 11632 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP. ACCESSION . VERSION . KEYWORDS pGI3EM11 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11632) AUTHORS Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C, Fritz-Laylin LK, Buchler NE TITLE Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology. JOURNAL Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741. PUBMED 32392127 REFERENCE 2 (bases 1 to 11632) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11632 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(1..1074) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(1503..2132) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" rep_origin 3293..4635 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind complement(4256..4303) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." primer_bind 4676..4694 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4897..5112 /gene="S. cerevisiae URA3" /label=URA3 promoter CDS 5113..5916 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" misc_feature 6083..6107 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 6288..6310 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6302..6319 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6303..6319 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 6618..7643 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA KE" CDS 7662..8375 /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" primer_bind complement(7689..7717) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 8312..8332 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" terminator 8395..8582 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(8450..8474) /label=pHybLex-R /note="ADH terminator, reverse primer" primer_bind complement(8597..8613) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8597..8613) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8610..8632) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8621..8637 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8645..8675) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8690..8711 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 8888..8912 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" primer_bind complement(9194..9213) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 9205..10020 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(9278..9297) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 10115..10703 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10604..10623 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10857..10874 /label=L4440 /note="L4440 vector, forward primer" misc_feature 10889..11029 /label=bom /note="basis of mobility region from pBR322" rep_origin 11373..11567 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" ORIGIN 1 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg 61 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag 121 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat 181 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag 241 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg 301 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc 361 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga 421 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt 481 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg 541 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt 601 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc 661 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg 721 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc 781 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa 841 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg 901 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc 961 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc 1021 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat 1081 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg 1141 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt 1201 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt 1261 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc 1321 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac 1381 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc 1441 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta 1501 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg 1561 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg 1621 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc 1681 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt 1741 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg 1801 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca 1861 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc 1921 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc 1981 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg 2041 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg 2101 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta 2161 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc 2221 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca 2281 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg 2341 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa 2401 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc 2461 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct 2521 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg 2581 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg 2641 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc 2701 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc 2761 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg 2821 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag 2881 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg 2941 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc 3001 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg 3061 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc 3121 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag 3181 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat 3241 gtcgttttca gaagacggct gcactgaacg tcagaaggaa aagtgccacc tgaacgaagc 3301 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa 3361 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg 3421 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa 3481 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac 3541 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt 3601 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct 3661 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt 3721 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct 3781 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg 3841 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat 3901 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta 3961 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata 4021 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag 4081 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg 4141 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt 4201 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt 4261 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac 4321 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc 4381 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta 4441 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc 4501 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag 4561 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc 4621 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag 4681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 4741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 4801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 4861 agagcagatt gtactgagag tgcaccatac caccttttca attcatcatt ttttttttat 4921 tctttttttt gatttcggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag 4981 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa 5041 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa 5101 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct 5161 gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg 5221 gatgttcgta ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt 5281 ttactaaaaa cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg 5341 ctaaaggcat tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac 5401 attggtaata cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca 5461 gacattacga atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg 5521 gcagaagaag taacaaagga acctagaggc cttttgatgc tagcagaatt gtcatgcaag 5581 ggctccctat ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa 5641 gattttgtta tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat 5701 tggttgatta tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag 5761 tatagaaccg tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga 5821 ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg 5881 gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca 5941 tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt acctggcatg 6001 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct 6061 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt 6121 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctcattcgc cattcaggct 6181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 6241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 6301 ttgtaaaacg acggccagtg ccaagctttt atgctccaag cggagacgat gaggccgata 6361 ttacagtggc ttagtagcgt gtacaatatt tccataaaag gctacatggt gagtttttca 6421 cgtgaccgta attagtccca attaggcttt attgtatatt gctactaagc atcgttaaat 6481 gacctgctcg aagcttcgtc tataaaaggc gggcgtgtgt gcgcttcccc cgctacgtct 6541 ttttcacaaa cactttgcga cttgaccaag acctttcacc cgcaccactc tcatccatca 6601 cacacacaaa ttaattaatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt 6661 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat 6721 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg 6781 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga 6841 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc 6901 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc 6961 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg 7021 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga 7081 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg 7141 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc 7201 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg 7261 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct 7321 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc 7381 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac 7441 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg 7501 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg 7561 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca 7621 gcactcgtcc gagggcaaag gaaggaggag gaggaggagg aagtaaagga gaagaacttt 7681 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt 7741 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt 7801 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc acttatggtg 7861 ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca 7921 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga 7981 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta 8041 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac 8101 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta 8161 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa 8221 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt 8281 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg 8341 ggattacaca tggcatggat gaactataca aataggcgcg ccacttctaa ataagcgaat 8401 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa 8461 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg 8521 taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc 8581 gggaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 8641 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 8701 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 8761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca 8821 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg 8881 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc 8941 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg 9001 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg 9061 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta 9121 acgatgacag agcgttgctg cctgtgatcc agatcatgaa caataaaact gtctgcttac 9181 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctctagg 9241 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 9301 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg 9361 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 9421 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 9481 gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa 9541 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 9601 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 9661 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 9721 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 9781 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 9841 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 9901 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 9961 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 10021 gaattaattc atgatcctgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 10081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 10141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 10201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 10261 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 10321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 10381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 10441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 10501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 10561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 10621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 10681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 10741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 10801 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 10861 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 10921 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 10981 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc 11041 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 11101 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 11161 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc 11221 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga 11281 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt 11341 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag 11401 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag 11461 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc 11521 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt 11581 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta ca //